A5063831500- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Chromatin Remodeling and Cancer
- Plant Molecular Biology Research
- DNA Repair Mechanisms
- Protein Degradation and Inhibitors
- CRISPR and Genetic Engineering
- Digital Marketing and Social Media
- Customer Service Quality and Loyalty
- Digital Communication and Language
- Cancer-related molecular mechanisms research
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Ubiquitin and proteasome pathways
University of California, San Francisco
2020-2023
Our understanding of the beads-on-a-string arrangement nucleosomes has been built largely on high-resolution sequence-agnostic imaging methods and sequence-resolved bulk biochemical techniques. To bridge divide between these approaches, we present single-molecule adenine methylated oligonucleosome sequencing assay (SAMOSA). SAMOSA is a high-throughput method that combines methyltransferase footprinting real-time DNA to natively nondestructively measure nucleosome positions individual...
Unlike other chromatin remodelers, INO80 preferentially mobilizes hexasomes, which can form during transcription. Why prefers hexasomes over nucleosomes remains unclear. Here, we report structures of
A developmentally regulated mechanism, mediated by a zinc finger protein Zscan4, protects genome integrity in early embryos.
Abstract Nearly all essential nuclear processes act on DNA packaged into arrays of nucleosomes. However, our understanding how these (for example, replication, RNA transcription, chromatin extrusion and nucleosome remodeling) occur individual remains unresolved. Here, to address this deficit, we present SAMOSA-ChAAT: a massively multiplex single-molecule footprinting approach map the primary structure individual, reconstituted templates subject virtually any chromatin-associated reaction. We...
Abstract Aberrant chromatin is expanding as a new class of disease and resistance driver for multiple pathologies. While billions dollars have been poured into drugging regulatory factors, the toxicity previous approaches limits potential promise. Conventional drug discovery strategies focused on targeting only stable interfaces regulators. However, specific regulation function occurs in large part from unstructured intrinsically disordered regions (IDRs). IDRs help specify functionality...
The intricate regulation of chromatin plays a key role in controlling genome architecture and accessibility. Histone lysine methyltransferases regulate by catalyzing the methylation specific histone residues but are also hypothesized to have equally important non-catalytic roles. SUV420H1 di- tri-methylates H4 20 (H4K20me2/me3) crucial roles DNA replication, repair, heterochromatin formation, is dysregulated several cancers. Many these processes were linked its catalytic activity. However,...
ABSTRACT ATP-dependent chromatin remodelers regulate the DNA accessibility required of virtually all nuclear processes. Biochemical studies have provided insight into remodeler action at nucleosome level, but how these findings translate to activity on fibers in vitro and vivo remains poorly understood. Here, we present a massively multiplex single-molecule platform allowing high-resolution mapping nucleosomes assembled mammalian genomic sequences. We apply this method distinguish between...
ABSTRACT Our understanding of the beads-on-a-string arrangement nucleosomes has been built largely on high-resolution sequence-agnostic imaging methods and sequence-resolved bulk biochemical techniques. To bridge divide between these approaches, we present single-molecule adenine methylated oligonucleosome sequencing assay (SAMOSA). SAMOSA is a high-throughput method that combines methyltransferase footprinting real-time DNA to natively nondestructively measure nucleosome positions...
Under the context of globalization, it is essential to understand cultural requirements and preferences a target culture if company wants develop website which can appropriately map that culture. Based on author’s previous study, culturally preferred characteristics between Taiwan UK have been found (Hsieh Hong, 2013). The finding formulates further questions: “Since different for web design attributes exist, how be applied increase usability?” Therefore, aim this paper measure effect use...