A5029461691- Respiratory viral infections research
- Influenza Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Pneumonia and Respiratory Infections
- Viral Infections and Vectors
- Congenital Diaphragmatic Hernia Studies
- Neonatal Respiratory Health Research
- Viral Infections and Immunology Research
- Virology and Viral Diseases
- Viral Infections and Outbreaks Research
- SARS-CoV-2 detection and testing
- Protein Structure and Dynamics
- Emergency and Acute Care Studies
- COVID-19 epidemiological studies
- Immune responses and vaccinations
- Travel-related health issues
- Receptor Mechanisms and Signaling
- Bacteriophages and microbial interactions
- Immune Response and Inflammation
- Animal Virus Infections Studies
- Tracheal and airway disorders
- CRISPR and Genetic Engineering
- Zoonotic diseases and public health
- Bacterial Infections and Vaccines
- Advanced biosensing and bioanalysis techniques
Peter Doherty Institute
2023-2025
The Royal Melbourne Hospital
2025
Tohoku University
2015-2024
Victorian Infectious Diseases Reference Laboratory
2023-2024
Australian Government
2023
Government of Western Australia Department of Health
2023
Research Institute for Tropical Medicine
2016-2022
Institute of Virology of the Slovak Academy of Sciences
2019
Niigata University
2008-2014
Phylogenetic analysis of respiratory syncytial virus (RSV) group B genotype BA strains from the 2002-2003 to 2009-2010 seasons collected in Niigata, Japan, revealed four distinct clusters, designated new genotypes BA7, BA8, BA9, and BA10. These were not associated with large outbreaks community.
Human respiratory syncytial virus (HRSV) is a major cause of acute lower tract infections in infants and children worldwide. We performed molecular analysis HRSV among with clinical diagnosis severe pneumonia four study sites the Philippines, including Biliran, Leyte, Palawan, Metro Manila from June 2012 to July 2013. Nasopharyngeal swabs were collected screened for using real-time polymerase chain reaction (PCR). Positive samples tested by conventional PCR sequenced second hypervariable...
ABSTRACT The emergence and widespread occurrence of antiviral drug-resistant seasonal human influenza A viruses, especially oseltamivir-resistant A/H1N1 virus, are major concerns. To understand the genetic background we performed full genome sequencing prepandemic strains. Seasonal including antiviral-susceptible amantadine-resistant obtained from several areas in Japan during 2007-2008 2008-2009 seasons were analyzed. Sequencing genomes these viruses was performed, phylogenetic...
Pandemic influenza A(H1N1) 2009 virus was first detected in Japan May and continued to circulate the 2010-2011 season. This study aims characterize human viruses circulating pandemic post-pandemic periods determine prevalence of antiviral-resistant viruses.Respiratory specimens were collected from patients with influenza-like illness on their visit at outpatient clinics during 2009-2010 seasons. Cycling probe real-time PCR assays performed screen for strains. Sequencing phylogenetic analysis...
Two novel G3P[4] rotavirus strains were detected from children with acute diarrhea in Sendai, Japan, identified as a G3–P[4]–I2–R2–C2–M2–A2–N2–T2–E2–H2 genotype constellation by whole-genome sequence analysis. The VP7 gene of the two displayed highest nucleotide identity (91 %) and showed close genetic relationship (99 % bootstrap value) to an equine reported India. other segments related human group A rotaviruses. This report suggests possible reassortment event between
Background: Whole-genome sequencing (WGS) provides critical insights into the Respiratory syncytial virus (RSV) transmission and any emerging mutations that could impair efficacy of monoclonal antibodies or vaccines have been recently licenced for clinical use worldwide. However, ability to sequence RSV genomes at large scale is limited by expensive time-consuming methods. Oxford Nanopore Technology (ONT) offers significant improvements in next generation (NGS) both turnaround time cost,...
<i>Objectives:</i> To perform genetic analysis of influenza A and B viruses in Myanmar from 2005 to 2007 determine the prevalence amantadine-resistant viruses. <i>Methods:</i> Phylogenies HA NA genes were analyzed mutations M2 that confer resistance amantadine screened. <i>Results:</i> Influenza exhibited seasonality, which coincided during rainy season June August. Out 2,618 samples, 76 132 isolated. Phylogenetic showed 2005, 11 A/H1N1 isolates formed one...
Abstract In 2007 and 2008 in Myanmar, we detected influenza viruses A (H3N2) that exhibited reduced sensitivity to both zanamivir amantadine. These rare naturally occurring harbored a novel Q136K mutation neuraminidase S31N M2.
ABSTRACT In eight successive seasons (2001 to 2009), a total of 726 human respiratory syncytial virus (HRSV) infections from 1,560 children with acute lower tract illness were identified. Molecular analysis the attachment (G) protein gene confirmed that 52 (7.8%) infected more than once any 3 genotypes HRSV-A (genotypes GA5, NA1, and NA2) and/or 6 HRSV-B BA4, BA5, BA7 BA10). Repeated in 46 cases (82.1%) occurred next season, only one case same season (10-day interval). First 33 (63.5%) 19...
Little is known about whether neuraminidase inhibitors are effective for children infected with oseltamivir-resistant influenza A(H1N1) viruses.Children aged 15 years and younger having influenza-like illness who visited outpatient clinics within 48 hours of fever onset were enrolled from 2006-2007 to 2008-2009 seasons in Japan. Patients received oseltamivir, zanamivir, or no treatment after screening by a rapid antigen test. Nasopharyngeal swabs collected before antiviral therapy used virus...
Pneumonia remains the leading cause of hospitalisations and deaths among children aged <5 years. Diverse respiratory pathogens acute infections, including pneumonia. Here, we analysed viral bacterial risk factors associated with death hospitalised children.A 9-year case series study.Two secondary-care hospitals, one tertiary-care hospital research centre in Philippines.5054 years severe pneumonia.Nasopharyngeal swabs for virus identification, venous blood samples culture were collected....
BackgroundNorovirus (NV) causes acute gastroenteritis in infants. Humoral and fecal immunoglobulin A (IgA) responses have been correlated with protection against NV; however, the role of breast milk IgA NV infection associated diarrhea is still unknown. This study aimed to evaluate protective NV-specific (NV-IgA) milk.MethodsNinety-five samples collected from mothers enrolled a 2016–2017 Peruvian birth cohort were tested for total NV-IgA by ELISA using GII·4 variants non-GII·4 genotype...
Rapid molecular testing for severe acute respiratory coronavirus 2 (SARS-CoV-2) variants may contribute to the development of public health measures, particularly in resource-limited areas. Reverse transcription recombinase polymerase amplification using a lateral flow assay (RT-RPA-LF) allows rapid RNA detection without thermal cyclers. In this study, we developed two assays detect SARS-CoV-2 nucleocapsid (N) gene and Omicron BA.1 spike (S) gene-specific deletion–insertion mutations...
We report highly pathogenic avian influenza A(H5N1) virus clade 2.3.2.1a in a child traveler returning to Australia from India. The was previously unreported reassortant consisting of 2.3.2.1a, 2.3.4.4b, and wild bird low pathogenicity gene segments. These findings highlight surveillance gaps South Asia.
ABSTRACT Amantadine is one of the antiviral agents used to treat influenza A virus infections, but resistant strains have widely emerged worldwide. In present study, we developed a novel method detect amantadine-resistant harboring Ser31Asn mutation in M2 gene based on cycling probe and real-time PCR. We also studied rate amantadine resistance 2007-2008 season Japan. Two different primer sets were designed for A/H1N1 A/H3N2 each single nucleotide polymorphism corresponding Ser/Asn at residue...
ABSTRACT Neuraminidase inhibitors are agents used against influenza viruses; however, the emergence of drug-resistant strains is a major concern. Recently, prevalence oseltamivir-resistant seasonal A (H1N1) virus increased globally and pandemic 2009 viruses was reported. In this study, we developed cycling probe real-time PCR method for detection viruses. We designed two sets primers probes that were labeled with 6-carboxyfluorescein or 6-carboxy-X-rhodamine to identify single nucleotide...
Community-acquired pneumonia (CAP) is a common cause of morbidity and mortality among adults worldwide. However, the distribution etiology CAP varies from one country to another, with limited data rural areas.A prospective hospital-based study on adult was conducted in Leyte, Central Philippines May 2010 2012. Blood, sputum, nasopharyngeal samples obtained patients were used identify pathogens using standard microbiological culture methods PCR.Of 535 enrolled, 38% younger than 50 years old....
The lack of vaccine and limited antiviral options against respiratory syncytial virus (RSV) highlights the need for novel therapeutic strategies. One alternative is to develop drugs that target host factors required viral replication. Several microarray proteomics studies had been published identify possible are affected during RSV In order obtain a comprehensive understanding RSV-host interaction, we integrated available proteome transcriptome datasets used it construct virus-host...
Human respiratory syncytial virus (RSV) is the leading cause of severe acute infection in infants and young children, which characterized by repeated infections. However, role amino acid substitutions infections remains unclear. Hence, this study aimed to elucidate genetic characteristics RSV children with using molecular analyses F G genes.
Abstract Background The limited treatment options for children with severe respiratory syncytial virus (RSV) infection highlights the need a comprehensive understanding of host cellular response during infection. We aimed to identify genes that are associated RSV disease and drugs can be repurposed Methods examined clinical data blood samples from 37 hospitalized (29 mild 8 severe) tested RNA using next-generation sequencing profile global mRNA expression processes. Results Retractions,...