A5047974251- Burkholderia infections and melioidosis
- Streptococcal Infections and Treatments
- Plant Pathogens and Fungal Diseases
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Antimicrobial Resistance in Staphylococcus
- Nail Diseases and Treatments
- Antifungal resistance and susceptibility
- Pneumonia and Respiratory Infections
- Bacterial Infections and Vaccines
- Fungal Infections and Studies
- Reproductive tract infections research
- Antibiotic Resistance in Bacteria
- Chemical Looping and Thermochemical Processes
- Infectious Diseases and Mycology
- Infective Endocarditis Diagnosis and Management
- Bacterial Identification and Susceptibility Testing
- Parasitic Infections and Diagnostics
- Vector-borne infectious diseases
- Parasites and Host Interactions
- Mycobacterium research and diagnosis
- Antibiotic Use and Resistance
- Vibrio bacteria research studies
- Vector-Borne Animal Diseases
- Syphilis Diagnosis and Treatment
Royal Darwin Hospital
2016-2025
Government of the Northern Territory
2014-2024
Australian Government
2022-2024
Government of Western Australia Department of Health
2020-2024
Breast Cancer Network Australia
2024
Royal Prince Alfred Hospital
2024
Northern Territory Health Services
2017-2023
Women's and Children's Health Network
2023
Menzies School of Health Research
2014-2022
Charles Darwin University
2020-2022
Nucleic acid detection methods such as PCR have become a common tool for microbial identification and diagnosis. Although amplification can be performed directly various cultures, filamentous fungi yeasts, prior isolation of DNA is often preferred. As the extraction process eliminates many unknown interfering substances present in biological material, it plays an important role ensuring consistent test results. Toward this end, considerable efforts been made to enable improved preparation...
Detection of microsporidia in clinical specimens has relied on electron microscopy, histology, or staining. This article describes further alterations to the modified trichrome staining method which make it easier identify microsporidial spores. The changes are a decrease phosphotungstic acid level and substitution colorfast counterstain, aniline blue, for fast green original stain. stain provides good contrast between spores background material including human fungal cells. Stool from 139...
Melioidosis is a severe disease that can be difficult to diagnose because of its diverse clinical manifestations and lack adequate diagnostic capabilities for suspected cases. There broad interest in improving detection diagnosis this not only melioidosis-endemic regions but also outside these melioidosis may underreported poses potential bioterrorism challenge public health authorities. Therefore, workshop academic, government, private sector personnel from around the world was convened...
Abstract We report the results of typings, for immunoglobulin G allotypes, 5392 Native Americans from ten samples, typings having been performed over last 20 years. Four cultural groups are represented: Pimans—Pima and Papago; Puebloans—Zuni Hopi; Pai—Walapai; Athabascans—Apache Navajo. The haplotype Gm 1;21 has highest frequency in each population while 1,2;21 is polymorphic all except Hopi. Mongoloid marker 1;11,13 found primarily Athabascans. Caucasian 3;5,11,13 at frequencies several...
Knowledge of contemporary epidemiology candidaemia is essential. We aimed to identify changes since 2004 in incidence, species and antifungal susceptibilities Candida spp. causing Australia.These data were collected from nationwide active laboratory-based surveillance for over 1 year (within 2014-2015). Isolate identification was by MALDI-TOF MS supplemented DNA sequencing. Antifungal susceptibility testing performed using Sensititre YeastOne™.A total 527 episodes (yielding 548 isolates)...
A fatal case of Japanese encephalitis (JE) occurred in a resident the Tiwi Islands, Northern Territory Australia February 2021, preceding large JE outbreak south-eastern 2022. This study reports detection, whole genome sequencing and analysis virus responsible (designated JEV/Australia/NT_Tiwi Islands/2021).Reverse transcription quantitative PCR (RT-qPCR) testing was performed on post-mortem brain specimens using range (JEV)-specific assays. Virus isolation from attempted by inoculation...
Objective: To compare the unprecedented 91 cases of melioidosis in Top End Northern Territory Australia from 1 October 2009 to 30 September 2010 with 540 preceding 20 years and postulate reasons for this year very high incidence. Design, setting participants: Review prospectively collected data on all patients culture-confirmed at Royal Darwin Hospital, End's tertiary referral centre, since 1989. Main outcome measures: Population-based annual incidence melioidosis; differences epidemiology,...
ABSTRACT Streptococcus pyogenes is commonly believed to be resistant trimethoprim-sulfamethoxazole (SXT), resulting in reservations about using SXT for skin and soft tissue infections (SSTI) where S. involved. ' vitro susceptibility depends on the medium's thymidine content. Thymidine allows bypass sulfur-mediated inhibition of folate metabolism and, historically, has resulted apparently reduced sulfur antibacterials. The low concentration Mueller-Hinton agar (MHA) now regulated. We explored...
Rabbit antisera evoked by purified pepsin-extracted group A streptococcal M proteins were screened for the presence of joint cross-reactive antibodies indirect immunofluorescence using thin sections mouse knee joints. Pep M1, M5, and M18 contained that cross-reacted with chondrocytes, cartilage, synovium. Immunofluorescence inhibition assays showed some epitopes shared among three heterologous serotypes protein. The pep M5 localized to different synthetic peptides C-terminal region M5....
Campylobacter cryaerophila was recovered from a single stool specimen of 35-year-old homosexual man who presented with intermittent diarrhea for 4 to 6 months. The isolate identified as C. by using biochemical reactions and confirmed DNA-DNA hybridization gas-liquid chromatograph profiles. base composition DNA the found be 31.1 +/- 1 mol% (G+C). previously reported associated bovine porcine abortions in animals. We believe this is first report recovery human stool.
The Darwin Prospective Melioidosis Study has documented 785 melioidosis cases over 23 years. Recurrent occurred in 39/679 (5.7%) patients surviving initial infection; 29 suffered relapse of the original infection, and 10 presented with a new Burkholderia pseudomallei infection. With improved therapy, become rare recent
Burkholderia pseudomallei, the causative agent of high-mortality disease melioidosis, is a gram-negative bacterium that naturally resistant to many antibiotics. There no vaccine for and effective eradication reliant on biphasic prolonged antibiotic administration. The carbapenem drug meropenem current gold standard option treating severe melioidosis. Intrinsic B. pseudomallei resistance toward has not yet been documented; however, could conceivably develop over course infection, leading...
Abstract Background Nocardia is an opportunistic pathogen that can cause life-threatening disease. We aimed to characterize the epidemiological, microbiological, and clinical features of nocardiosis in tropical north Australia. Methods conducted a retrospective cohort study diagnosed between 1997 2014. Population-based incidences were calculated using district population data. Results Clinically significant was identified 61 patients. The unadjusted population-based annual incidence 2.02...
Indigenous populations globally are disproportionately affected by chronic hepatitis B virus (HBV) infection however contemporary sero-prevalence data often absent. In the population of Northern Territory (NT) Australia unique C4 sub-genotype HBV universally circulates. There no studies sero-prevalence, nor impact vaccination program (which has a serotype mismatch compared to C4), at population-wide level.We examined all available serology results obtained from three main laboratories...
Hemodialysis is a risk factor for Staphylococcus aureus bloodstream infection (SAB). In this single-center study, SAB rates were 56% lower during the monsoonal wet season when patients on hemodialysis receive supervised melioidosis prophylaxis with trimethoprim-sulfamethoxazole. This intervention may reduce in high-risk patients; however, further targeted studies are required.