A5009529163- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Use and Resistance
- Antimicrobial Resistance in Staphylococcus
- Pneumocystis jirovecii pneumonia detection and treatment
- Antifungal resistance and susceptibility
- Mycobacterium research and diagnosis
- Genomics and Phylogenetic Studies
- Urinary Tract Infections Management
- Fungal Infections and Studies
- Machine Learning in Healthcare
- Plant Pathogenic Bacteria Studies
- Tuberculosis Research and Epidemiology
- Salmonella and Campylobacter epidemiology
- COVID-19 Clinical Research Studies
- Electronic Health Records Systems
- SARS-CoV-2 and COVID-19 Research
- Clostridium difficile and Clostridium perfringens research
- Cytomegalovirus and herpesvirus research
- Escherichia coli research studies
- Renal Transplantation Outcomes and Treatments
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Polyomavirus and related diseases
Monash University
2018-2025
The Alfred Hospital
2013-2025
Alfred Health
2013-2025
Impact
2024
Australian Regenerative Medicine Institute
2024
Epworth Hospital
2021-2022
Columbia University Irving Medical Center
2015-2020
Austin Hospital
2015-2019
Osaka Prefectural Institute of Public Health
2019
Heidelberg University
2016-2017
ABSTRACT Ceftazidime-avibactam (CAZ-AVI) is a promising novel treatment for infections caused by carbapenem-resistant Enterobacteriaceae (CRE). Despite improved outcomes compared to those achieved with aminoglycoside- and colistin-based regimens, the rapid evolution of CAZ-AVI resistance during has previously been reported in Klebsiella pneumoniae sequence type 258 (ST258) bla KPC-3 -harboring isolates. Here, we report stepwise isolation two phenotypically distinct CAZ-AVI-resistant isolates...
Knowledge of contemporary epidemiology candidaemia is essential. We aimed to identify changes since 2004 in incidence, species and antifungal susceptibilities Candida spp. causing Australia.These data were collected from nationwide active laboratory-based surveillance for over 1 year (within 2014-2015). Isolate identification was by MALDI-TOF MS supplemented DNA sequencing. Antifungal susceptibility testing performed using Sensititre YeastOne™.A total 527 episodes (yielding 548 isolates)...
Recent reports have established the escalating threat of carbapenem-resistant
Abstract Infections by multidrug-resistant bacteria (MDRB) remain a leading cause of morbidity and mortality after liver transplantation (LT). Gut dysbiosis characteristic end-stage disease may predispose patients to intestinal MDRB colonization infection, in turn exacerbating dysbiosis. However, relationships between LT unclear. We prospectively recruited 177 adult undergoing at single tertiary care center. 16 S V3-V4 rRNA sequencing was performed on 723 fecal samples collected pre-LT...
Abstract Infections caused by metallo-beta-lactamase-producing organisms (MBLs) are a global health threat. Our understanding of transmission dynamics and how MBLs establish endemicity remains limited. We analysed two decades bla IMP-4 evolution in hospital using sequence data from 270 clinical environmental isolates (including 169 completed genomes) identified the gene across 7 Gram-negative genera, 68 bacterial strains distinct plasmid types. showed an initial multi-species outbreak...
Multidrug-resistant organisms (MDROs) are an important cause of morbidity and mortality after solid organ transplantation. We aimed to characterize MDRO colonization dynamics infection in liver transplant (LT) recipients through innovative use active surveillance whole-genome sequencing (WGS). prospectively enrolled consecutive adult patients undergoing LT from March 2014 2016. Fecal samples were collected at multiple timepoints time enrollment 12 months posttransplant. Samples screened for...
Susceptibility testing of the polymyxins (colistin and polymyxin B) is challenging for clinical laboratories. The Clinical Laboratory Standards Institute (CLSI) Antimicrobial Testing Subcommittee evaluated two methods to enable accurate these agents. These were a colistin broth disk elution (CBDE) agar test (CAT), latter which was using inoculum volumes, 1 μl (CAT-1) 10 (CAT-10). collection 270 isolates Enterobacterales, 122 Pseudomonas aeruginosa isolates, 106 Acinetobacter spp. isolates....
Abstract Infections remain important contributors to mortality in hematopoietic stem cell transplantation ( HSCT ). Method We studied the evolving epidemiology and trends susceptibility of bacterial C andida isolates at an A ustralian center. total 528 s 508 patients were performed from pril 2001 M ay 2010. 605 eligible for study inclusion; 318 (53%) gram‐positive, 268 (44%) gram‐negative, 19 (3%) species. Results The most common site was blood (380 isolates, 63%). S taphylococcus aureus...
Polymyxins are antimicrobials of last resort for the treatment carbapenem-resistant Enterobacteriaceae, but resistance in 5% to >40% isolates has been reported. We conducted a genomic survey clinical polymyxin-resistant (PR) Klebsiella pneumoniae determine molecular mechanisms PR and role polymyxin exposure versus transmission emergence.We included 88 patients with K. from 2011-2018 collected demographic, antimicrobial exposure, infection data. Whole-genome sequencing was performed on 388...
Polymyxins are last-resort antibiotics used to treat highly resistant Gram-negative bacteria. There increasing reports of polymyxin resistance emerging, raising concerns a postantibiotic era. Polymyxin is therefore significant public health threat, but current phenotypic methods for detection difficult and time-consuming perform. have been efforts use whole-genome sequencing antibiotic resistance, this has apply because its complex polygenic nature. The significance our research that we...
Important culture-change initiatives (e.g. improving hand hygiene compliance) are frequently associated with variable uptake among different healthcare worker (HCW) categories. Inherent personality differences between these groups may explain change and help improve future intervention design.We used an innovative personality-profiling tool (ColourGrid®) to assess standard HCW categories at five large Australian hospitals using two data sources (HCW participant surveys [PS] generic...
Objective: To review cases of nosocomial influenza and compare the epidemiology, clinical characteristics outcomes with community-acquired cases. Design, setting participants: Prospective case series adults hospitalised during April – November 2010 2011 using a hospital-based sentinel surveillance system. A was defined as polymerase chain reaction-confirmed where symptom onset more than 2 days after admission or, if this not known, date positive test 7 admission. Main outcome measures:...
Treatment options for prostatitis caused by multidrug-resistant gram-negative bacilli are limited. We report two cases cured with oral fosfomycin and provide a pharmacokinetic analysis of predose concentrations during treatment.
Polymyxin resistance (PR) threatens the treatment of carbapenem-resistant Klebsiella pneumoniae (CRKP) infections. PR frequently arises through chemical modification lipid A portion lipopolysaccharide. Various mutations are implicated in PR, including three two-component systems—CrrA/B, PmrA/B, and PhoP/Q—and negative regulator MgrB. Few have been functionally validated. Therefore, here we adapt a CRISPR-Cas9 system to CRKP elucidate how clinical isolates induce PR. We demonstrate that CrrB...
Understanding the changing epidemiology of Staphylococcus aureus bacteremia, as well variables associated with poor outcomes, can yield insight into potential interventions.This study was a retrospective, observational cohort adult patients at an academic medical center in New York City who had S. bloodstream infections between 1 January 2007 and 31 December 2015. Participants were divided 3 periods: group (2007-2009), 2 (2010-2012), (2013-2015) for trend analysis. All clinical strains...
The adoption of electronic health records (EHRs) has created opportunities to analyse historical data for predicting clinical outcomes and improving patient care. However, non-standardised representations anomalies pose major challenges the use EHRs in digital research. To address these challenges, we have developed EHR-QC, a tool comprising two modules: standardisation module preprocessing module. migrates source EHR standard format using advanced concept mapping techniques, surpassing...
Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) is widely used in clinical microbiology laboratories for bacterial identification but its use detection antimicrobial resistance (AMR) remains limited. Here, we MALDI-TOF MS with artificial intelligence (AI) approaches to successfully predict AMR
Abstract Background Staphylococcus aureus skin and soft tissue infections (SA-SSTIs) are common in healthcare community settings, recurrences occur at variable frequency, even after successful initial treatment. Knowing the exact burden timing of recurrent disease is critical to planning evaluating interventions prevent SSTIs. Methods In this retrospective study, SSTI cases patients aged ≥18 years 3 US medical centers (Columbia, Chicago, Vanderbilt) between 2006 2016 were analyzed according...