This study aims to evaluate the feasibility of a label-free nanobiosensor based on blood plasma surface-enhanced Raman spectroscopy (SERS) method for exploring variability different tumor (T) stages in nasopharyngeal cancer (NPC). Au nanoparticles as SERS-active nanostructures were directly mixed with human enhance scattering signals. High quality SERS spectra can be acquired from samples belong 60 healthy volunteers, 25 NPC patients T1 stage and 75 T2–T4 stage. A diagnostic accuracy 83.5%...

Abstract Cisplatin remains to be primary chemotherapeutic drug for gastric cancer patients, especially advanced stage ones. However, or acquired resistance often occurs with the mechanisms being not well understood, which results in relapse of and poor survival. Herein, we found that HER2 upregulation was associated cisplatin resistance. We observed cisplatin-resistant cells underwent a morphological change similar epithelial-mesenchymal transition (EMT) is mediated by overexpression. When...

The receptor tyrosine kinase-like orphan receptors (ROR) family contains the atypical member ROR1, which plays an oncogenic role in several malignant tumors. However, clinical potentials and underlying mechanisms of ROR1 gastric cancer progression remain largely unknown. In this study, we validated microRNA-mediated gene repression mechanism involved ROR1. Bioinformatic prediction, luciferase reporter assay, quantitative real-time PCR (qRT-PCR) western blotting were used to reveal regulatory...

Sphingosine-1-phosphate (S1P) is a bioactive lysophospholipid that involves in numerous pathophysiological processes. Endothelial progenitor cells (EPCs) play crucial role endothelial repair and tumor angiogenesis. The aim of study was to determine the effects S1P on proliferation anti-apoptosis EPCs their signaling pathways. In this study, we showed S1P, SEW2871 (a selective receptor 1 (S1PR1) agonist), or CYM5541 3 (S1PR3) allosteric agonist promotes attenuates apoptosis bone marrow...

A surface-enhanced Raman spectroscopy (SERS) based on silver nanoparticle technology was applied to analyze and classify human blood plasma with the aim develop a simple label-free test for esophageal cancer detection. High quality SERS spectra in range of 400-1800 cm(-1) can be acquired from 36 patients 50 healthy volunteers' samples. Tentative assignments bands indicated specific biomolecular changes associated transformation, including an increase relative amounts nucleic acid...

Considering the characteristics of complex nonlinear and multiple response variables a super-high dam, kernel partial least squares (KPLS) method, as strongly multivariate analysis is introduced into field dam safety monitoring for first time. A universal unified optimization algorithm designed to select key parameters KPLS method obtain optimal (OKPLS). Then, OKPLS used establish model identify abnormal behavior via fusion diagnosis. An deformation data arch was undertaken case study....

In this study, an electroporation-based surface-enhanced Raman scattering (SERS) technique was employed to differentiate the human myeloid leukemia cells from normal bone marrow mononuclear with aim develop a fast and label-free method for cell screening.The Ag nanoparticles were delivered into living by electroporation, then high quality SERS spectra successfully obtained 60 acute promyelocytic (HL60 line), chronic myelogenous (K562 line) (BMC).Principal component analysis (PCA) combined...

Apoptosis of nasopharyngeal carcinoma cells (C666 cell line) induced by an anticancer drug cisplatin was investigated confocal Raman micro-spectroscopy using near-infrared laser (785 nm) excitation in this study. The spectra C666 treated with different concentrations (0.5, 1, 5 and 10 μg mL−1) for 24 h treatment times (6, 12, 18 h) mL−1 were collected separately. Difference the intensities peaks assigned to DNA band (783 cm−1, 1338 1523 cm−1 1576 cm−1) between control becomes greater as...

The purpose of this study was to develop a more powerful blood analysis method based on polarized surface enhanced Raman spectroscopy (SERS) technology for non-invasive and sensitive colorectal cancer (CRC) detection. efficiency different scattering signals (non-polarization, parallel polarization perpendicular polarization) serum SERS explored the first time. Results demonstrated that explore distinctive spectral differences between normal groups. And higher diagnostic accuracy 91.6% could...

The clinical significance of ABO blood typing extends beyond transfusion medicine and is demonstrated to be associated with susceptibility various diseases, even including cancer. In this study, a home-made laser tweezers Raman spectroscopy (LTRS) system was applied detect red cells (RBCs) the aim develop label-free, simple objective method for first time. High-quality spectra RBCs in fingerprint region 420-1700 cm-1 can obtained, meanwhile exciting results achieved, especially an accuracy...

The existing electroporation method can rapidly deliver nanoparticles (NPs) into living cells for intracellular surface-enhanced Raman scattering (SERS) imaging. Unfortunately, the cellular SERS signals are major from molecules located near two poles of cell facing toward to electrodes because most NPs enter through these and easily happen aggregate there. Here, we present an optimized transferring obtain a uniform distribution. distribution was monitored by signal 4-mercaptobenzoic acid,...

Chemodynamic therapy (CDT), employing metal ions to transform endogenous H2O2 into lethal hydroxyl radicals (•OH), has emerged as an effective approach for tumor treatment. Yet, its efficacy is diminished by glutathione (GSH), commonly overexpressed in tumors. Herein, a breakthrough strategy involving extracellular vesicle (EV) mimetic nanovesicles (NVs) encapsulating iron oxide nanoparticles (IONPs) and β-Lapachone (Lapa) was developed amplify intracellular oxidative stress. The...

This paper presents the use of high spatial resolution silver nanoparticle based near-infrared surface enhanced Raman scattering (SERS) from rat pancreatic tissue to obtain biochrmical information about tissue. A quality SERS signal a mixture tissues and nanoparticles can be obtained within 10 s using Renishaw micro-Raman system. Prominent bands were assigned known molecular vibrations, such as vibrations DNA bases, RNA proteins lipids. Different structures diabetic normal have...

Endothelial progenitor cells (EPCs) play important roles in the regeneration of vascular endothelial (ECs). Platelet-derived growth factor receptor (PDGFR)-β is known to contribute proliferation, migration, and angiogenesis EPCs, this study aims investigate effects transplantation EPCs overexpressing PDGFR-β on regeneration. We transplanted genetically modified into a mouse model with carotid artery injury. After 3 days transplantation, enhanced green fluorescent protein (EGFP)-expressing...

Electrical pulse-mediated enhanced silver nanoparticles delivery is a much better method for intracellular surface-enhanced Raman spectroscopy (SERS) measurements of suspension cells. Robust and high-quality SERS spectra living cells were obtained based on an electroporation-SERS method, which can overcomes the shortcoming non-uniform distribution localized in cell cytoplasm after electroporation reduces amount variance delivered into different The parameters include three 150 V (375 V/cm)...

Endothelial progenitor cells (EPCs) play a fundamental role in neoangiogenesis and tumor angiogenesis. Through the sphingosine-1-phosphate receptor 3 (S1PR3), (S1P) can stimulate functional capacity of EPCs. Platelet-derived growth factor receptor-beta (PDGFR-β) contributes to migration angiogenesis This study aimed investigate whether S1P induces EPCs through S1PR3/PDGFR-β/Akt signaling pathway. We used Transwell system Chemicon In Vitro Angiogenesis Assay Kit with CAY10444 (an S1PR3...

An accurate understanding of biomolecular changes in living cells associated with malignant transformation is paramount importance early cancer detection. The aim this study was to apply near-infrared Raman spectroscopy (RS) for differentiating from normal cells. High-quality spectra the range 450–1800 cm−1 can be obtained 31 and 64 hematological malignancy including 43 CA46 21 U266 There were significant differences between groups, which suggests special percentage biomolecules lipid,...

A surface-enhanced Raman spectroscopy (SERS) method was developed for the analysis of traditional Chinese medicine 'Atractylodis Macrocephalae Rhizoma' pieces (AMRP) first time with aim to develop a quick detection. Both spectra and SERS were obtained from AMRP, tentative assignments bands in measured suggested that only few weak peaks could be observed regular spectra, while primary at around 536, 555, 619, 648, 691, 733, 790, 958, 1004, 1031, 1112, 1244, 1324, 1395, 1469, 1574 1632 cm−1...

We have evaluated the capabilities of surface-enhanced Raman scattering (SERS) technology for analyzing two Huo–Xue–Hua–Yu decoctions (HXHYDs) prepared according to different prescriptions. The aim this study was evaluate relevance SERS applied decoction traditional Chinese medicines (TCM). HXHYD I original prescription; same preparation method used II, except crudeweight ratio described in prescription. There no signal conventional spectra HXHYDs. Silver nanoparticles were directly mixed...