A5069409410- Cancer, Hypoxia, and Metabolism
- Neuroblastoma Research and Treatments
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Genomics, phytochemicals, and oxidative stress
- Adipose Tissue and Metabolism
- Autophagy in Disease and Therapy
- Glioma Diagnosis and Treatment
Columbia University Irving Medical Center
2023
Weatherford College
2023
Stanford University
2020-2022
Despite the fact that Otto H. Warburg discovered effect almost one hundred years ago, why cancer cells waste most of glucose carbon as lactate remains an enigma. proposed a connection between and cell dedifferentiation. Hypoxia is common tumor microenvironmental stress induces blocks differentiation. The underlying mechanism by which this occurs poorly understood, no effective therapeutic strategy has been developed to overcome resistance Using neuroblastoma differentiation model, we hypoxia...
Abstract The Warburg effect is the major metabolic hallmark of cancer. According to himself, consequence cell dedifferentiation. Therefore, reversing might be an approach restore differentiation in In this study, we used a mitochondrial uncoupler, niclosamide ethanolamine (NEN), activate respiration, which induced neural neuroblastoma cells. NEN treatment increased NAD+/NADH and pyruvate/lactate ratios also α-ketoglutarate/2-hydroxyglutarate (2-HG) ratio. Consequently, promoter CpG island...
The interplay between metabolism and chromatin signaling is implicated in cancer progression. However, whether how metabolic reprogramming tumors generates vulnerabilities remain unclear. Lung adenocarcinoma (LUAD) frequently harbor aberrant activation of the NRF2 antioxidant pathway, which drives aggressive chemo-resistant disease. Using a chromatin-focused CRISPR screen, we report that sensitizes LUAD cells to genetic chemical inhibition class I histone deacetylases (HDACs). This...
<div>Abstract<p>The Warburg effect is the major metabolic hallmark of cancer. According to himself, consequence cell dedifferentiation. Therefore, reversing might be an approach restore differentiation in In this study, we used a mitochondrial uncoupler, niclosamide ethanolamine (NEN), activate respiration, which induced neural neuroblastoma cells. NEN treatment increased NAD<sup>+</sup>/NADH and pyruvate/lactate ratios also α-ketoglutarate/2-hydroxyglutarate (2-HG)...
<p>Supplementary Data</p>
<p>Primers for p53 mutation analysis</p>
<p>Primers for p53 mutation analysis</p>
<p>Regional counting of differential methylated probes in SK-N-BE(2) cells treated with NEN for 24hrs under nomoxia. Go enrichment pathways the CpG Island promoter (B) (C) and gene body (D) (E)</p>
<p>Supplementary Data</p>
<p>SK-N-BE(2) cells were treated with NEN for 16hrs. (A) Upregulated and (B) downregulated genes from RNA-seq data (n=3) (sleuth q-value < 0.05 fold change estimate b > abs(ln(2))). (C) The top 10 gene ontology (GO) pathways enriched upregulated by using DAVID analysis. (D) analysis.</p>
<p>SK-N-BE(2) cells were treated with NEN for 16hrs. (A) Upregulated and (B) downregulated genes from RNA-seq data (n=3) (sleuth q-value < 0.05 fold change estimate b > abs(ln(2))). (C) The top 10 gene ontology (GO) pathways enriched upregulated by using DAVID analysis. (D) analysis.</p>
<p>Regional counting of differential methylated probes in SK-N-BE(2) cells treated with NEN for 24hrs under nomoxia. Go enrichment pathways the CpG Island promoter (B) (C) and gene body (D) (E)</p>
<p>Favorable and (B) unfavorable gene list (p-value<0.05) from 11 available neuroblastoma databases R2 (https://hgserver1.amc.nl/cgi-bin/r2/main.cgi) (C) Overlapped the favorable prognosis sets (p-value<0.05, number >1000) 7 (https://hgserver1.amc.nl/cgi-bin/r2/main.cgi). (D) (E) Go analysis of overlapped sets. (F) (G) GSEA Favorable genes RNA-seq experiments in Figure (1D).</p>
<p>Favorable and (B) unfavorable gene list (p-value<0.05) from 11 available neuroblastoma databases R2 (https://hgserver1.amc.nl/cgi-bin/r2/main.cgi) (C) Overlapped the favorable prognosis sets (p-value<0.05, number >1000) 7 (https://hgserver1.amc.nl/cgi-bin/r2/main.cgi). (D) (E) Go analysis of overlapped sets. (F) (G) GSEA Favorable genes RNA-seq experiments in Figure (1D).</p>
<div>Abstract<p>The Warburg effect is the major metabolic hallmark of cancer. According to himself, consequence cell dedifferentiation. Therefore, reversing might be an approach restore differentiation in In this study, we used a mitochondrial uncoupler, niclosamide ethanolamine (NEN), activate respiration, which induced neural neuroblastoma cells. NEN treatment increased NAD<sup>+</sup>/NADH and pyruvate/lactate ratios also α-ketoglutarate/2-hydroxyglutarate (2-HG)...
Abstract Interplay between metabolism and chromatin signaling have been implicated in cancer initiation progression. However, whether how metabolic reprogramming tumors generates specific epigenetic vulnerabilities remain unclear. Lung adenocarcinoma (LUAD) frequently harbor mutations that cause aberrant activation of the NRF2 antioxidant pathway drive aggressive chemo-resistant disease. We performed a chromatin-focused CRISPR screen report sensitized LUAD cells to genetic chemical...
Abstract Dysregulated DNA methylation is associated with poor prognosis in cancer patients, promoting tumorigenesis and therapeutic resistance 1 . methyltransferase inhibitors (DNMTi) reduce promote cell differentiation, two DNMTi already approved for treatment 2 However, these drugs rely on division to dilute existing methylation, thus the ‘demethylation’ effects are achieved a passive manner, limiting their application slow-proliferating tumor cells. In this study we use mitochondrial...