A5055382987- Cancer, Hypoxia, and Metabolism
- Neuroblastoma Research and Treatments
- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Adipose Tissue and Metabolism
Weatherford College
2023
Stanford University
2022
Abstract The Warburg effect is the major metabolic hallmark of cancer. According to himself, consequence cell dedifferentiation. Therefore, reversing might be an approach restore differentiation in In this study, we used a mitochondrial uncoupler, niclosamide ethanolamine (NEN), activate respiration, which induced neural neuroblastoma cells. NEN treatment increased NAD+/NADH and pyruvate/lactate ratios also α-ketoglutarate/2-hydroxyglutarate (2-HG) ratio. Consequently, promoter CpG island...
<div>Abstract<p>The Warburg effect is the major metabolic hallmark of cancer. According to himself, consequence cell dedifferentiation. Therefore, reversing might be an approach restore differentiation in In this study, we used a mitochondrial uncoupler, niclosamide ethanolamine (NEN), activate respiration, which induced neural neuroblastoma cells. NEN treatment increased NAD<sup>+</sup>/NADH and pyruvate/lactate ratios also α-ketoglutarate/2-hydroxyglutarate (2-HG)...
<p>Supplementary Data</p>
<p>Primers for p53 mutation analysis</p>
<p>Primers for p53 mutation analysis</p>
<p>Regional counting of differential methylated probes in SK-N-BE(2) cells treated with NEN for 24hrs under nomoxia. Go enrichment pathways the CpG Island promoter (B) (C) and gene body (D) (E)</p>
<p>Supplementary Data</p>
<p>SK-N-BE(2) cells were treated with NEN for 16hrs. (A) Upregulated and (B) downregulated genes from RNA-seq data (n=3) (sleuth q-value < 0.05 fold change estimate b > abs(ln(2))). (C) The top 10 gene ontology (GO) pathways enriched upregulated by using DAVID analysis. (D) analysis.</p>
<p>SK-N-BE(2) cells were treated with NEN for 16hrs. (A) Upregulated and (B) downregulated genes from RNA-seq data (n=3) (sleuth q-value < 0.05 fold change estimate b > abs(ln(2))). (C) The top 10 gene ontology (GO) pathways enriched upregulated by using DAVID analysis. (D) analysis.</p>
<p>Regional counting of differential methylated probes in SK-N-BE(2) cells treated with NEN for 24hrs under nomoxia. Go enrichment pathways the CpG Island promoter (B) (C) and gene body (D) (E)</p>
<p>Favorable and (B) unfavorable gene list (p-value<0.05) from 11 available neuroblastoma databases R2 (https://hgserver1.amc.nl/cgi-bin/r2/main.cgi) (C) Overlapped the favorable prognosis sets (p-value<0.05, number >1000) 7 (https://hgserver1.amc.nl/cgi-bin/r2/main.cgi). (D) (E) Go analysis of overlapped sets. (F) (G) GSEA Favorable genes RNA-seq experiments in Figure (1D).</p>
<p>Favorable and (B) unfavorable gene list (p-value<0.05) from 11 available neuroblastoma databases R2 (https://hgserver1.amc.nl/cgi-bin/r2/main.cgi) (C) Overlapped the favorable prognosis sets (p-value<0.05, number >1000) 7 (https://hgserver1.amc.nl/cgi-bin/r2/main.cgi). (D) (E) Go analysis of overlapped sets. (F) (G) GSEA Favorable genes RNA-seq experiments in Figure (1D).</p>
<div>Abstract<p>The Warburg effect is the major metabolic hallmark of cancer. According to himself, consequence cell dedifferentiation. Therefore, reversing might be an approach restore differentiation in In this study, we used a mitochondrial uncoupler, niclosamide ethanolamine (NEN), activate respiration, which induced neural neuroblastoma cells. NEN treatment increased NAD<sup>+</sup>/NADH and pyruvate/lactate ratios also α-ketoglutarate/2-hydroxyglutarate (2-HG)...