A5088860415- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Effects and risks of endocrine disrupting chemicals
- Pluripotent Stem Cells Research
- Birth, Development, and Health
- CRISPR and Genetic Engineering
- Renal and related cancers
- Assisted Reproductive Technology and Twin Pregnancy
- Epigenetics and DNA Methylation
- Ovarian function and disorders
- Virus-based gene therapy research
- Phytoestrogen effects and research
- Circadian rhythm and melatonin
- Image and Video Stabilization
- Microplastics and Plastic Pollution
- HVDC Systems and Fault Protection
- Image Enhancement Techniques
- Seed Germination and Physiology
- Toxic Organic Pollutants Impact
- Sesame and Sesamin Research
- Livestock Management and Performance Improvement
- melanin and skin pigmentation
- GABA and Rice Research
- Liver Disease Diagnosis and Treatment
Nantong University
2023-2024
Guangxi University
2015-2024
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2015-2021
Oxford University Press (United Kingdom)
2020
Beijing Institute of Graphic Communication
2015
Lanzhou University
2012
Postovulatory ageing compromises oocyte quality and subsequent development in various manners.We aimed to assay the protective effects of mogroside V on porcine during vitro explore related causes.We observed that can effectively maintain normal morphology early embryo competence after prolonged culture for 24 h.Moreover, markedly reduce reactive oxygen species (ROS) levels, alleviate spindle formation chromosome alignment abnormalities, improve mitochondrial contents, adenosine triphosphate...
Prolonged culture of metaphase II oocytes is an in vitro aging process that compromises oocyte quality. We tested whether melatonin preserves epigenetic modifications after prolonged culture. The porcine were maturated for 44 h, and then continuously cultured medium supplemented with or without 24 h. found the parthenogenetic blastocyst formation rate prolonged-culture was lower than fresh oocytes. further observed methylation at H3K4me2 H3K27me2 enhanced However, 5mc fluorescence intensity...
Abstract Induction of repeated superovulation with exogenous hormones is widely used in assisted reproductive technology (ART). Though it generally safe, emerging evidence has indicated that may compromise oocyte quality. However, few studies have explored how to ameliorate such impairment. Because melatonin beneficial influences on oocytes various detrimental environments, we aimed explore whether could protect mouse after superovulation. We found markedly reduced meiotic maturation and...
Accumulating evidence has demonstrated that lipopolysaccharide (LPS) compromises female reproduction, especially oocyte maturation and competence. However, methods to protect quality from LPS-induced deterioration remain largely unexplored. We previously found mogroside V (MV) can promote embryonic development. whether MV alleviate the adverse effects of LPS exposure on is unclear. Thus, in this study, we used porcine oocytes as a model explore administration meiotic defects. Our findings...
In this study, we attempted to establish a culture system for in vitro spermatogenesis from spermatogonial stem cells (SSCs) of Bama mini-pig.Dissociated testicular 1-month-old pigs were co-cultured mimic vivo spermatogenesis.The seeded minimum essential medium alpha (-MEM) supplemented with Knockout serum replacement (KSR).Three-dimensional colonies formed after 10 days culture.The showed positive staining SSC-associated markers such as UCHL1, PLZF, THY1, OCT4, Dolichos biflorus...
Somatic cloning, also known as somatic cell nuclear transfer (SCNT), is a promising technology which has been expected to rapidly extend the population of elaborately selected breeding boars with superior production performance. Chinese Guike No. 1 pig breed novel swine specialized strain incorporated pedigree background Duroc and Luchuan breeds, thus inherits an excellent The present study was conducted establish cloning procedures adult from strain. Ear skin fibroblasts were first isolated...
Accumulating evidence has demonstrated that benzo(a)pyrene (BaP) exposure adversely affects female reproduction, especially oocyte meiotic maturation and subsequent embryo development. Although we previously found mogroside V (MV), a major bioactive component of S. grosvenorii , can protect oocytes from quality deterioration caused by certain stresses, whether MV alleviate BaP exposure-mediated defects remains unknown. In this study, mice were exposed to treated concomitantly with gavage. We...
Sex control technology is of great significance in the production domestic animals, especially for rapidly breeding water buffalo (bubalus bubalis), which served as a research model present study. We have confirmed that fluorescence protein integrated into Y chromosome fit sexing pre-implantation embryos mouse. Firstly, we optimized efficiency targeted integration exogenous gene encoding enhanced green fluorescent (eGFP) and mCherry Neuro-2a cells, mouse embryonic stem cells (NIH3T3), fetal...
Abstract Although numerous attempts have been made to alter the sex ratio of progeny mammals, limitations current technologies prevented their widespread use in farm animals. The presence or absence a Y chromosome determines whether mammalian embryo develops as male female, and non-invasive genetic reporters such fluorescence protein markers intensively applied variety fields research. To develop instantaneous method for advance determination embryos, we developed chromosome-linked eGFP...
Introduction Epididymal lumen fluids provides a stable microenvironment for sperm maturation. Ca 2+ binding protein CABS1 is known to maintain structural integrity of mouse flagella during epididymal transit sperm. Besides, was reported contain anti-inflammatory peptide sequences and be present in both human saliva plasma. However, little about the role regulation fluids. Methods To further confirm epididymis, we identified expression Moreover, high performance liquid chromatography, coupled...
Oocyte maturation involves both nuclear and cytoplasmic maturation. Mogroside V (MV) has been shown to enhance maturation, mitochondrial content, developmental potential of porcine oocyte during in vitro (IVM). However, the impact MV on its underlying mechanisms are not understood. This study aimed assess effect Germinal vesicle (GV) oocytes treated with exhibited a noticeable increase cortical granules (CGs) formation. Additionally, enhanced expression NNAT improved glucose uptake mature...
The calcium-binding protein spermatid-associated 1 (CABS1) localizes to the principal piece of mature sperm flagella. Deletion CABS1 results in subfertility male mice, possibly due an impaired annulus However, it is unknown whether there are other mechanisms by which affects fertility. Our current investigation has uncovered that located midsection flagellum testicular and epididymal sperm. Moreover, mice lacking exhibit a defect progressive motility Furthermore, regulation calcium levels,...