A5015488107- Wound Healing and Treatments
- Electrospun Nanofibers in Biomedical Applications
- Skin Protection and Aging
- Dermatologic Treatments and Research
- Silk-based biomaterials and applications
- Skin and Cellular Biology Research
- 3D Printing in Biomedical Research
- Burn Injury Management and Outcomes
- melanin and skin pigmentation
- Surgical Sutures and Adhesives
- Corneal Surgery and Treatments
- Advancements in Transdermal Drug Delivery
- Tissue Engineering and Regenerative Medicine
- Planarian Biology and Electrostimulation
- Mesenchymal stem cell research
- Tendon Structure and Treatment
- Hair Growth and Disorders
- Antimicrobial Peptides and Activities
- Dermatology and Skin Diseases
- Nerve injury and regeneration
- Nonmelanoma Skin Cancer Studies
- Bone Tissue Engineering Materials
- Bee Products Chemical Analysis
- Cell Adhesion Molecules Research
- Essential Oils and Antimicrobial Activity
Sheffield Hallam University
2023-2024
University of Cincinnati Medical Center
2009-2023
Shriners Hospitals for Children - Cincinnati
2012-2023
University of Cincinnati
2012-2022
Avidity (United States)
2020
The Ohio State University
2018
University of California, Davis
2003-2016
UC Davis Comprehensive Cancer Center
2016
Schlumberger (British Virgin Islands)
2016
Shriners Hospitals for Children
1993-2014
The significance of melanotropic hormones as physiologic regulators cutaneous pigmentation in humans is still controversial. Until recently, no direct effect for melanotropins could be demonstrated on human melanocytes. Here we present conclusive evidence that alpha-melanotropin (alpha-melanocyte-stimulating hormone, alpha-MSH) and the related hormone corticotropin (adrenocorticotropic ACTH) stimulate proliferation melanogenesis melanocytes maintained culture a growth medium lacking any AMP...
Antibodies which bind to different nuclear antigens in tissue sections or permeabilized cell cultures are useful markers of subsets connective disease, especially lupus erythematosus (LE), but whether these antibodies able react with intracellular sequestered vivo and cause immunologic damage has been a matter much debate. We report experiments show that ultraviolet light-irradiated, cultured human keratinocytes IgG from the sera LE patients either monospecific anti-SSA/Ro, anti-RNP, anti-Sm...
Engineered human skin is commonly fabricated using collagen scaffolds that often have poor mechanical properties. To improve the strength of collagen-based scaffolds, poly(caprolactone) (PCL) was blended with and formed into submicron fibers electrospinning. At concentrations < 10% PCL (M(PCL)/[M(Collagen) + M(PCL)] x 100), component evenly distributed within matrix. Increasing to 30% caused separation phases forming local domains Tensile testing indicated 10-100% significantly improved...
Abstract Collagen and glycosaminoglycan (GAG) dermal skin substitutes (membranes) were studied as substrates for cultured human epidermal keratinocytes. Structure of was optimized pore size to promote ingrowth fibrovascular tissue from the wound bed culture keratinocytes membrane's surface. Pore freeze‐dried material regulated by control temperature freezing between −50°C −20°C concentration starting materials 0.17% 1.62% wt/vol. A nonporous surface collagen‐GAG laminated membranes provide a...
Comparison of cultured skin substitutes (CSSs) and split-thickness autograft (STAG) was performed to assess whether the requirement for autologous grafts may be reduced in treatment massive burns.Cultured consisting collagen-glycosaminoglycan substrates populated with fibroblasts keratinocytes have been demonstrated close full-thickness wounds athymic mice express normal antigens after closure excised burn patients.Data were collected from 17 patients between days 2 14 determine incidence...
Prompt and permanent closure of excised full-thickness burns remains a critical factor in patient's recovery from massive burn injuries. Hypothetically, Integra Artificial Skin (Integra) may replace the need for allografts immediate wound coverage, cultured skin substitutes (CSS) that contain stratified epithelium autografts definitive closure. To test this hypothesis, 3 patients with greater than 60% their total body surface areas had eschar within 14 days admission. was applied, biopsy...
Objective Comparison of cultured skin substitutes (CSS) and split-thickness autograft (AG) was performed to assess whether donor-site harvesting can be reduced quantitatively functional cosmetic outcome is similar qualitatively in the treatment patients with massive cutaneous burns. Summary Background Data Cultured consisting collagen-glycosaminoglycan substrates populated autologous fibroblasts keratinocytes have been shown close full-thickness wounds preclinical clinical studies acceptable...
Abstract Porous, nowoven fibrous gelatin scaffolds were prepared using electrospinning. Electrospun with varying fiber diameter, interfiber distance, and porosity fabricated by altering the concentration of electrospinning solution. Solution was a significant predictor higher solution correlated larger diameters distances. The potential electrospun as scaffolding material for dermal epidermal tissue regeneration also evaluated. Interfiber distances >5.5 μm allowed deeper penetration human...
Journal Article Summary of the 2012 ABA Burn Quality Consensus Conference Get access Nicole S. Gibran, MD, FACS, FACS Search for other works by this author on: Oxford Academic Google Scholar Shelley Wiechman, PhD, ABPP, ABPP Walter Meyer, MD Linda Edelman, RN, PhD Jim Fauerbach, Gibbons, MS, MS Radha Holavanahalli, Carly Hunt, MA, MA Kelly Keller, RN Elizabeth Kirk, MSN, APN, APN ... Show more Jacqueline Laird, BSN, Giavonni Lewis, Sidonie Moses, BSN Jill Sproul, Gretta Wilkinson, Steve...
Stable closure of full-thickness burn wounds remains a limitation to recovery from burns greater than 50% the total body surface area (TBSA). Hypothetically, engineered skin substitutes (ESS) consisting autologous keratinocytes and fibroblasts attached collagen-based scaffolds may reduce requirements for donor skin, decrease mortality. ESS were prepared split-thickness biopsies collected after enrollment 16 pediatric patients into an approved study protocol. autograft (AG) applied 15...
Cultured skin substitutes (CSS) consisting of autologous fibroblasts and keratinocytes combined with biopolymers are an adjunctive treatment for large excised burns. CSS containing two cell types limited by anatomical deficiencies, including lack a vascular plexus, leading to slower vascularization after grafting than split-thickness autograft. To address this limitation, were prepared human keratinocytes, fibroblasts, dermal microvascular endothelial cells (HDMEC) isolated from single...
A serum-free, hormone-supplemented medium, enriched with a mixture of lipids, has been developed that supports rapid clonal growth human diploid fetal lung fibroblasts (Flow 2000, WI-38, MRC-5, and IMR-90) low-passage foreskin fibroblasts. The which contains less than 1 microgram total protein per ml, also serial passage Flow 2000 cells under totally serum-free conditions. It provides lipid at 10 micrograms/ml as liposome prepared from soybean lecithin, cholesterol, sphingomyelin, vitamin E,...
Abstract Keloids are disfiguring scars that extend beyond the original wound borders and resist treatment. exhibit excessive extracellular matrix deposition, although underlying mechanisms remain unclear. To better understand molecular basis of keloid scarring, here we define genomic profiles fibroblasts keratinocytes. In both cell types, keloid‐derived cells differential expression genes encompassing a diverse set functional categories. Strikingly, keratinocytes exhibited decreased...