A5101924208- Wound Healing and Treatments
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Burn Injury Management and Outcomes
- Protease and Inhibitor Mechanisms
- Silk-based biomaterials and applications
- Surgical Sutures and Adhesives
- Electrospun Nanofibers in Biomedical Applications
- Pressure Ulcer Prevention and Management
- Tendon Structure and Treatment
- Proteoglycans and glycosaminoglycans research
- 3D Printing in Biomedical Research
- Periodontal Regeneration and Treatments
- Skin and Cellular Biology Research
- Curcumin's Biomedical Applications
- Dermatologic Treatments and Research
- Platelet Disorders and Treatments
- Blood properties and coagulation
- Contact Dermatitis and Allergies
- Angiogenesis and VEGF in Cancer
- Dermatology and Skin Diseases
- Hair Growth and Disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Genetic and Kidney Cyst Diseases
- Diabetic Foot Ulcer Assessment and Management
Stony Brook School
1997-2024
Stony Brook University
2015-2024
State University of New York
1999-2021
Stony Brook Medicine
2020
NeoMatrix Therapeutics (United States)
2019
Forth Valley Royal Hospital
2018
In-Q-Tel
2011-2013
Stony Brook University Hospital
2011-2013
Centre for Biomedical Engineering and Physics
2005-2009
Estée Lauder (United States)
2005
Angiogenesis depends on the adhesive interactions of vascular cells. The adhesion receptor integrin α v β 3 was identified as a marker angiogenic tissue. Integrin expressed blood vessels in human wound granulation tissue but not normal skin, and it showed fourfold increase expression during angiogenesis chick chorioallantoic membrane. In latter assay, monoclonal antibody to blocked induced by basic fibroblast growth factor, tumor necrosis factor-α, melanoma fragments had no effect...
ABSTRACT After injury, the wound space is filled with a fibrin/fibronectin clot containing growth factors released by platelets and monocytes. In response to these factors, fibroblasts migrate into fibrin contribute formation of granulation tissue. The functional mechanisms allowing leave collagenous matrix normal connective tissue invade provisional have not been fully defined. To investigate we established new in vitro model which simulates specific aspects early healing, that is,...
Hyaluronan (HA) hydrogels resist attachment and spreading of fibroblasts most other mammalian cell types. A thiol-modified HA (3,3'-dithiobis(propanoic dihydrazide) [HA-DTPH]) was modified with peptides containing the Arg-Gly-Asp (RGD) sequence then crosslinked polyethylene glycol (PEG) diacrylate (PEGDA) to create a biomaterial that supported attachment, spreading, proliferation. The were evaluated in vitro vivo three assay systems. First, behavior human murine on surface evaluated....
The fibronexus ( FNX ), a very close transmembrane association of individual extracellular fibronectin fibers and actin microfilaments, was found previously at the substrate-binding surface fibroblasts in tissue culture (Singer, 1. 1., 1979, Cell, 16:675-685). To determine whether might be involved fibroblast adhesion during wound healing vivo, we looked for co-localization granulation formed within full-thickness guinea pig skin wounds. At 7-9 d, most were observed to coincident with...
Extracellular matrix (ECM) and growth factors are potent regulators of cell phenotype. These biological mediators cellular responses potentially interactive as such could drive cells through progressive phenotypes to create new tissue in morphogenesis wound repair. In fact, ECM composition changes during formation accompanied by alterations migration. How the regulate activities is poorly defined. To address this question repair, we cultured normal human dermal skin fibroblasts relaxed...
During wound repair, fibroblasts accumulate in the injured area until any defect is filled with stratified layers of cells and matrix. Such fibroplasia also occurs many fibrotic disorders. Transforming growth factor-β (TGF-β), a promotor granulation tissue vivo extracellular matrix production vitro, expressed during active healing fibroproliferative diseases. Under usual culture conditions, normal grow to confluence then cease proliferation. In this study, conditions TGF-β1 have been...
ABSTRACT Transforming growth factor-β, a potent modulator of cell function, induces fibroblasts cultured on plastic to increase collagen synthesis. In 5- and 7-day porcine skin wounds, which have minimal moderate matrix, respectively, transforming factor-β type I procollagen were coordinately expressed throughout the granulation tissue. However, in 10-day collagen-rich tissue expression diminished despite persistence factor-β. To investigate whether matrix attenuates collagen-synthetic...
The effects of exposure human dermal fibroblasts to rutile and anatase TiO(2) nanoparticles are reported. These particles can impair cell function, with the latter being more potent at producing damage. decreases area, proliferation, mobility, ability contract collagen. Individual shown penetrate easily through membrane in absence endocytosis, while some endocytosis is observed for larger particle clusters. Once inside, sequestered vesicles, which continue fill up increasing incubation time...
Fibronectin (FN) facilitates dermal fibroblast migration during normal wound healing. Proteolytic degradation of FN in chronic wounds hampers Previously, three functional domains (FNfd) have been shown to be sufficient for optimal adult human migration. Here we report the development an acellular hydrogel matrix comprised FNfds coupled a hyaluronan (HA) backbone stimulate repair. Employing Michael-type addition, cysteine- tagged were first homobifunctional PEG derivative. Thereafter, these...
During the time of tissue repair that ensues subsequent to injury, blood vessel wall fibronectin increases concomitantly with endothelial proliferation and angiogenesis. However, source this had not been delineated. In report we have demonstrated microvascular is produced in situ by proliferating vessels surrounding excisional wounds. This finding was established extirpating 3 mm skin from center a well-healed rat xenograph on flanks immunosuppressed mice, harvesting injured sites at various...
Journal Article Summary of the 2012 ABA Burn Quality Consensus Conference Get access Nicole S. Gibran, MD, FACS, FACS Search for other works by this author on: Oxford Academic Google Scholar Shelley Wiechman, PhD, ABPP, ABPP Walter Meyer, MD Linda Edelman, RN, PhD Jim Fauerbach, Gibbons, MS, MS Radha Holavanahalli, Carly Hunt, MA, MA Kelly Keller, RN Elizabeth Kirk, MSN, APN, APN ... Show more Jacqueline Laird, BSN, Giavonni Lewis, Sidonie Moses, BSN Jill Sproul, Gretta Wilkinson, Steve...