A5101451254- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Cholesterol and Lipid Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Peroxisome Proliferator-Activated Receptors
- Cell Adhesion Molecules Research
- Hydrogels: synthesis, properties, applications
- RNA Interference and Gene Delivery
- Alzheimer's disease research and treatments
- Silk-based biomaterials and applications
- HER2/EGFR in Cancer Research
- Drug Transport and Resistance Mechanisms
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Neuroscience and Neuropharmacology Research
- Cellular transport and secretion
- Photonic and Optical Devices
- Protein Degradation and Inhibitors
- MicroRNA in disease regulation
- Virus-based gene therapy research
- Pancreatic function and diabetes
- GaN-based semiconductor devices and materials
- Developmental Biology and Gene Regulation
- Protein Kinase Regulation and GTPase Signaling
- RNA Research and Splicing
Shanghai Jiao Tong University
2023-2024
XinHua Hospital
2024
Zhuhai People's Hospital
2022-2024
University of Science and Technology of China
2024
Army Medical University
2021-2024
Nanjing University
2008-2024
State Key Laboratory of Pollution Control and Resource Reuse
2024
Halmstad University
2024
Wuhan University of Technology
2020-2023
Ruijin Hospital
2023
Tangier disease, a condition characterized by low levels of high density lipoprotein and cholesterol accumulation in macrophages, is caused mutations the ATP-binding cassette transporter ABC1. In cultured ABC1 mRNA was induced an additive fashion 22(R)-hydroxycholesterol 9-cis-retinoic acid (9CRA), suggesting induction nuclear hormone receptors liver X receptor (LXR) retinoid (RXR) family. We cloned 5′-end human transcript from cholesterol-loaded THP1 macrophages. When transfected into RAW...
A new disulfide cross-linking strategy was developed to prepare hyaluronic acid (HA) hydrogel from thiol-modified HA. First, dithiobis(propanoic dihydrazide) (DTP) and dithiobis(butyric (DTB) were synthesized then coupled HA with carbodiimide chemistry. Next, bonds of the initially formed gel reduced using dithiothreitol (DTT) give, after exhaustive dialysis, corresponding macromolecular derivatives HA-DTPH HA-DTBH. The degree substitution HA-DTBH could be controlled 20% 70% available...
Cardiac magnetic resonance imaging (CMR) is the gold standard for cardiac function assessment and plays a crucial role in diagnosing cardiovascular disease (CVD). However, its widespread application has been limited by heavy resource burden of CMR interpretation. Here, to address this challenge, we developed validated computerized interpretation screening diagnosis 11 types CVD 9,719 patients. We propose two-stage paradigm consisting noninvasive cine-based followed cine late gadolinium...
The cholesterol ester transfer protein (CETP) facilitates the of HDL esters from plasma to liver. Transgenic mice expressing human CETP, controlled by its natural flanking region, increase expression this gene in response hypercholesterolemia. We established a CETP promoter-luciferase reporter assay differentiated 3T3-L1 adipocytes map sterol upregulatory element. Promoter mutagenesis suggested that direct repeat nuclear receptor binding sequence separated 4 nucleotides (DR4 element, –384...
Hyaluronan (HA) hydrogels resist attachment and spreading of fibroblasts most other mammalian cell types. A thiol-modified HA (3,3'-dithiobis(propanoic dihydrazide) [HA-DTPH]) was modified with peptides containing the Arg-Gly-Asp (RGD) sequence then crosslinked polyethylene glycol (PEG) diacrylate (PEGDA) to create a biomaterial that supported attachment, spreading, proliferation. The were evaluated in vitro vivo three assay systems. First, behavior human murine on surface evaluated....
A cell-targeted prodrug was developed for the anti-cancer drug Taxol, using hyaluronic acid (HA) as carrier. HA−Taxol bioconjugates were synthesized by linking Taxol 2'-OH via a succinate ester to adipic dihydrazide-modified HA (HA-ADH). The coupling of Taxol-NHS and HA-ADH provided several with different levels ADH modification loadings. fluorescent BODIPY−HA also illustrate cell targeting uptake chemically modified confocal microscopy. conjugates showed selective toxicity toward human...
A cell-targeted polymeric prodrug prepared from Taxol and chemically modified hyaluronic acid (HA) was evaluated in vitro. Herein we report four results support of the selective uptake targeted toxicity HA-Taxol prodrug. First, a fluorescently labeled (FITC-HA-Taxol) synthesized used to demonstrate cell-specific binding using flow cytometry confocal microscopy. Second, cytotoxicity FITC-HA-Taxol allowed direct correlation with cytotoxicity. Third, rapid bioconjugates could be blocked by...
We used molecular cloning and functional analyses to extend the family of Neu differentiation factors (NDFs) explore biochemical activity different NDF isoforms. Exhaustive revealed existence six distinct fibroblastic pro-NDFs, whose basic transmembrane structure includes an immunoglobulin-like motif epidermal growth factor (EGF)-like domain. Structural variation is confined three domains: C-terminal portion EGF-like domain (isoforms alpha beta), adjacent juxtamembrane stretch 1 4),...
The EGF-CFC gene Cripto encodes an extracellular protein that has been implicated in the signaling pathway for transforming growth factor beta (TGF) ligand Nodal.Although recent findings frog and fish embryos have suggested proteins function as coreceptors Nodal, studies cell culture a factor-like molecule.Here we reconcile these apparently disparate models of by using mammalian assay to investigate activities Nodal proteins.Using luciferase reporter assay, found consistent with its being...
The human cholesteryl ester transfer protein (CETP) transfers esters from high density lipoproteins to triglyceride-rich lipoproteins, indirectly facilitating uptake by the liver. Hepatic CETP gene expression is increased in response dietary hypercholesterolemia, an effect that mediated activity of liver X receptor/retinoid receptor (LXR/RXR) on a direct repeat 4 element CETPpromoter. In this study we show orphan nuclear LRH-1 also transactivates promoter binding proximal distinct DR4 site....
LADII (leukocyte adhesion deficiency type II)/CDGIIc (congenital disorder of glycosylation IIc) is a rare autosomal recessive disease characterized by leukocyte as well severe neurological and developmental abnormalities. It caused mutations in the Golgi GDP-fucose transporter, resulting reduction fucosylated antigens on cell surface. A recent study using fibroblasts from LADII/CDGIIc patients suggested that although terminal fucosylation N-glycans reduced severely, protein O-fucosylation...
O-Fucose is an unusual form of glycosylation found on epidermal growth factor-like (EGF) repeats and thrombospondin type 1 (TSRs) in many secreted transmembrane proteins. Recently O-fucose EGF was shown to play important roles Notch signaling. In contrast, physiological for TSRs are unknown. the accompanying paper (Luo, Y., Nita-Lazar, A., Haltiwanger, R. S. (2006) J. Biol. Chem. 281, 9385–9392), we demonstrated that enzyme distinct from protein O-fucosyltransferase adds TSRs. A known...
Liver X receptor/retinoid receptor (LXR/RXR) transcription factors have been found to induce a number of genes involved in the regulation cellular cholesterol efflux, including ATP-binding cassette transporter A1 (ABCA1), which mediates active efflux and phospholipids extracellular acceptors, such as apolipoprotein A-I (apoA-I). In screen for macrophage LXR/RXR target genes, we identified stearoyl-CoA desaturases 1 2 (Scd1 Scd2), subsequently tested hypothesis that SCD activity might...
Epidermal growth factor-like (EGF) repeats and thrombospondin type 1 (TSRs) are both small cysteine-knot motifs known to be O-fucosylated. The enzyme responsible for the addition of O-fucose EGF repeats, protein O-fucosyltransferase (POFUT1), has been identified shown essential in Notch signaling. Fringe, an beta1,3-N-acetylglucosaminyltransferase, elongates on specific from form a disaccharide that can further elongated tetrasaccharide. TSRs found many extracellular matrix proteins involved...
Abstract Age is a significant contributor to the onset of AD. Senolysis has been recently demonstrated ameliorate aging‐associated diseases that showing great potential in AD therapy. However, due presence BBB, anti‐AD activity senolytics are significantly diminished. SSK1 prodrug can be activated by β‐gal, lysosomal enzyme commonly upregulated senescent cells, and thus selectively eliminates cells. Furthermore, level β‐gal correlated with conventional genes from clinical sequencing data....
β-Site amyloid precursor protein-cleaving enzyme 1 (BACE1) is a membrane-bound aspartic protease that cleaves protein to produce neurotoxic peptide, Aβ, and implicated in triggering the pathogenesis of Alzheimer disease. We previously reported BACE1 cleaved rat β-galactoside α2,6-sialyltransferase (ST6Gal I) was overexpressed COS cells NH2 terminus ST6Gal I secreted from (E41 form) Glu41. Here we report gene knock-out mice have one third as much plasma control mice, indicating major which...