A5077931850- Virus-based gene therapy research
- HIV Research and Treatment
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Herpesvirus Infections and Treatments
- Veterinary Oncology Research
- T-cell and Retrovirus Studies
- Animal Genetics and Reproduction
- Animal Disease Management and Epidemiology
- Erythrocyte Function and Pathophysiology
- interferon and immune responses
- RNA Research and Splicing
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Cytokine Signaling Pathways and Interactions
- Genomics and Chromatin Dynamics
- Hepatitis B Virus Studies
- Immune Cell Function and Interaction
- Cell death mechanisms and regulation
- Monoclonal and Polyclonal Antibodies Research
- Xenotransplantation and immune response
- Cancer-related Molecular Pathways
- HIV/AIDS drug development and treatment
- Vector-Borne Animal Diseases
- RNA and protein synthesis mechanisms
Yamaguchi University
2012-2024
The University of Tokyo
1993-2017
Ministry of the Environment
2017
National Institute for Environmental Studies
2017
Veterinary Medical Center
2017
In-Q-Tel
2011
Tokyo Medical and Dental University
2006-2009
National Cancer Institute
1993-2007
Frederick National Laboratory for Cancer Research
2000-2007
National Institutes of Health
2000-2003
Severe acute respiratory syndrome (SARS) is characterized by rapidly progressing failure resembling acute/adult distress (ARDS) associated with uncontrolled inflammatory responses. Here, we demonstrated that, among five accessory proteins of SARS coronavirus (SARS-CoV) tested, 3a/X1 and 7a/X4 were capable activating nuclear factor kappa B (NF-kappaB) c-Jun N-terminal kinase (JNK), significantly enhanced interleukin 8 (IL-8) promoter activity. Furthermore, expression in A549 cells production...
Endogenous retroviruses (ERVs) comprise a significant percentage of the mammalian genome, and it is poorly understood whether they will remain as inactive genomes or emerge infectious retroviruses. Although several types ERVs are present in domestic cats, have not been demonstrated. Here, we report previously uncharacterized class endogenous gammaretroviruses, termed ERV-DCs, that hereditary cat genome. We characterized subset ERV-DC proviral clones, which numbered according to their genomic...
The host defense against viral infection is acquired during the coevolution or symbiosis of and pathogen. Several cellular factors that restrict retroviral have been identified in hosts. Feline leukemia virus (FeLV) a gammaretrovirus classified into several receptor interference groups, including novel FeLV-subgroup D (FeLV-D) we recently identified. FeLV-D generated by transduction env gene feline endogenous domestic cat (ERV-DCs) FeLV. Some ERV-DCs are replication competent viruses which...
The genetic basis of feline leukemia virus (FeLV)-induced lymphoma was investigated in a series 63 lymphoid tumors and tumor cell lines presumptive T-cell origin. These were examined for virus-induced rearrangements the c-myc, flvi-2 (bmi-1), fit-1, pim-1 loci, receptor (TCR) gene rearrangements, presence env recombinant FeLV (FeLV-B). myc locus most frequently affected naturally occurring lymphomas (32%; n = 38) either by transduction (21%) or proviral insertion (11%). Proviral insertions...
Hemoplasma (hemotropic mycoplasma) often causes hemolytic anemia in infected cats, especially those with immune suppression. An updated nationwide epidemiological survey of feline hemoplasmosis was conducted Japan. Blood samples were collected from 1,770 outdoor-accessing cats March to October 2008. The infections molecularly detected by PCR analyses, which are able distinguish Mycoplasma haemofelis (Mhf), `Candidatus M. haemominutum' (CMhm), and turicensis' (CMt) infections. Of the 468...
Feline leukemia virus (FeLV) belongs to the genus Gammaretrovirus, and causes a variety of neoplastic non-neoplastic diseases in cats. Alteration viral env sequences is thought be associated with disease specificity, but way which genetic diversity FeLV contributes generation such variants nature poorly understood. We isolated genes from naturally infected cats Japan analyzed evolutionary dynamics these genes. Phylogenetic reconstructions separated our samples into three distinct clusters,...
ABSTRACT Feline leukemia virus (FeLV) is an exogenous retrovirus that causes malignant hematopoietic disorders in domestic cats, and its virulence may be closely associated with viral sequences. FeLV classified into several subgroups, including A, B, C, D, E, T, based on receptor interference properties or usage. However, the transmission manner disease specificity of recombinant viruses FeLV-D FeLV-B remain unclear. The aim this study was to understand recombination events between...
The Friend spleen focus-forming virus (SFFV) encodes a unique envelope glycoprotein, gp55, which allows erythroid cells to proliferate and differentiate in the absence of erythropoietin (Epo). SFFV gp55 has been shown interact with Epo receptor complex, causing constitutive activation various signal-transducing molecules. When injected into adult mice, induces rapid erythroleukemia, susceptibility being determined by host gene Fv-2, was recently be identical encoding tyrosine kinase Stk/Ron....
Feline leukemia virus (FeLV) subgroups have emerged in infected cats via the mutation or recombination of env gene subgroup A FeLV (FeLV-A), primary virus. We report isolation and characterization a novel gene, TG35-2, that TG35-2 pseudotype can be categorized as subgroup. The envelope protein displays strong sequence identity to FeLV-A Env, suggesting selection pressure causes emerge.
ABSTRACT The erythroleukemia-inducing Friend spleen focus-forming virus (SFFV) encodes a unique envelope glycoprotein which allows erythroid cells to proliferate and differentiate in the absence of erythropoietin (Epo). In an effort understand how SFFV causes Epo independence, we have been examining rendered factor independent by infection for constitutive activation signal-transducing molecules. Previous studies from our laboratory showed that various molecules known be activated Epo,...
Abstract Feline leukemia virus (FeLV) clone33 was obtained from a domestic cat with acute myeloid (AML). The long terminal repeat (LTR) of this virus, like the LTRs present in FeLV other cats AML, differs known that it has 3 tandem direct 47‐bp repeats upstream region enhancer (URE). Here, we injected and found 41% developed myelodysplastic syndromes (MDS) characterized by peripheral blood cytopenias dysplastic changes bone marrow. Some MDS eventually AML. marrow majority induced produced...
Endogenous retroviruses (ERVs) of domestic cats (ERV-DCs) are one the youngest feline ERV groups in (Felis silvestris catus); some members replication competent (ERV-DC10, ERV-DC18, and ERV-DC14), produce antiretroviral soluble factor Refrex-1 (ERV-DC7 ERV-DC16), or can generate recombinant leukemia virus (FeLV). Here, we investigated ERV-DC European wildcats silvestris) detected four loci: ERV-DC6, ERV-DC7, ERV-DC14, ERV-DC16. ERV-DC14 was at a high frequency wildcats; however, it defective...
Host genetic resistance to viral infection controls the pathogenicity and epidemic dynamics of infectious diseases. Refrex-1 is a restriction factor against feline leukemia virus subgroup D (FeLV-D) an endogenous retrovirus (ERV) in domestic cats (ERV-DC). encoded by subset ERV-DC loci with truncated envelope genes secreted from cells as soluble protein. Here, we identified copper transporter CTR1 entry receptor for FeLV-D genotype I ERV-DCs. We also primate ERVs crab-eating macaques rhesus...
An updated nation-wide epidemiological survey of feline immunodeficiency virus (FIV) infection was conducted in Japan. Blood samples were collected from 1,770 outdoor accessing cats March to October 2008. Serologically, 410 (23.2%) positive for anti-FIV antibody. Proviral DNA the FIV env V3-V5 region isolated 348 cases could be phylogenetically analyzed. The present study disclosed a geographic distribution four subtypes (A, B, C and D) Even though an vaccine introduced Japan, we do not...
ABSTRACT Endogenous retroviruses (ERVs) are remnants of ancestral retroviral infections germ cells. Retroviral endogenization is an adaptation process for the host genome, and ERVs gradually attenuated or inactivated by mutation. However, some that have been “domesticated” their hosts eventually gain physiological functions, such as placentation viral resistance. We previously reported discovery Refrex-1, a soluble antiretroviral factor in domestic cats specifically inhibits infection feline...
Endogenous retroviruses (ERVs) are the remnants of ancient retroviral infections germ cells. Previous work identified one youngest feline ERV groups, ERV-DC, and reported that two ERV-DC loci, ERV-DC10 ERV-DC18 (ERV-DC10/DC18), can replicate in cultured Here, we another replication-competent provirus, ERV-DC14, on chromosome C1q32. ERV-DC14 differs from ERV-DC10/DC18 its phylogeny, receptor usage, and, most notably, transcriptional activities; although cells, it cannot establish a persistent...
Gammaherpesviruses (GHVs) are members of an emerging subfamily the family Herpesviridae. A recent study identified a novel GHV in domestic cats (Felis catus GHV1, FcaGHV1), and epidemiological surveys have found that FcaGHV1 is distributed worldwide. In this study, we investigated prevalence GHVs Japan with molecular survey. Blood samples were collected from 1,738 GHV-derived DNA was detected PCR 1.3% (23/1,738) Japanese cats. The very similar to cat North America. Older age (>5 years old)...
Friend spleen focus-forming virus (SFFV) causes rapid erythroleukemia in mice due to expression of its unique envelope glycoprotein, gp55. Erythroid cells expressing SFFV gp55 proliferate the absence their normal regulator erythropoietin (Epo) because constitutive activation Epo signal transduction pathways. Although infects many cell types, deregulation growth occurs only when erythroid cells, suggesting that these express proteins requires mediate biological effects. Not do receptor...
SUMMARY Cats are known to be the main reservoir for Bartonella henselae and clarridgeiae , which agents of ‘cat-scratch disease’ in humans. In present study, we investigated prevalence two species on 1754 cat bloods collected from all prefectures Japan during 2007–2008 by a nested-polymerase chain reaction (PCR) targeting 16S–23S rRNA internal transcribed spacer region. Overall, DNA was detected 4·6% (80/1754) cats examined. The nested-PCR showed that 48·8% (39/80) positive were infected...