A5102897005- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Bone and Dental Protein Studies
- Collagen: Extraction and Characterization
- Dental Implant Techniques and Outcomes
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- Enterobacteriaceae and Cronobacter Research
- Virus-based gene therapy research
- Escherichia coli research studies
- Cleft Lip and Palate Research
- HIV Research and Treatment
- Herpesvirus Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Otolaryngology and Infectious Diseases
- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Animal Disease Management and Epidemiology
- Cancer-related gene regulation
- Legionella and Acanthamoeba research
- Connective tissue disorders research
- Xenotransplantation and immune response
- Water Treatment and Disinfection
Hiroshima Prefectural Hospital
2018-2024
Fujifilm (Japan)
2018-2023
Osaka Prefectural Institute of Public Health
2019-2023
Hiroshima Minami High School
2020
Ministry of the Environment
2017
National Institute for Environmental Studies
2017
Yamaguchi University
2013-2017
Rakuno Gakuen University
1994-1995
The Legionella Reference Center in Japan collected 427 clinical isolates between 2008 and 2016, including 7 representative from corresponding outbreaks. collection included 419 pneumophila isolates, of which 372 belonged to serogroup 1 (SG1) (87%) the others SG2 SG15 except for SG7 SG11, 8 other species (Legionella bozemanae, dumoffii, feeleii, longbeachae, londiniensis, rubrilucens). L. were genotyped by sequence-based typing (SBT) represented 187 sequence types (STs), 126 occurred a single...
The host defense against viral infection is acquired during the coevolution or symbiosis of and pathogen. Several cellular factors that restrict retroviral have been identified in hosts. Feline leukemia virus (FeLV) a gammaretrovirus classified into several receptor interference groups, including novel FeLV-subgroup D (FeLV-D) we recently identified. FeLV-D generated by transduction env gene feline endogenous domestic cat (ERV-DCs) FeLV. Some ERV-DCs are replication competent viruses which...
Zoonotic pathogen Escherichia albertii has been identified as the cause of several human disease outbreaks; however, factors such general symptoms and incubation period E. infection have yet to be defined. Therefore, we aimed determine unique aspects outbreaks in Japan examine genetic characteristics causative pathogen. We studied all known that occurred up until 2015, which consisted five confirmed one putative outbreak (Outbreaks 1–6). Outbreaks were re-examined based on personal...
Feline leukemia virus (FeLV) subgroups have emerged in infected cats via the mutation or recombination of env gene subgroup A FeLV (FeLV-A), primary virus. We report isolation and characterization a novel gene, TG35-2, that TG35-2 pseudotype can be categorized as subgroup. The envelope protein displays strong sequence identity to FeLV-A Env, suggesting selection pressure causes emerge.
In this study, we developed porous medium cross-linked recombinant collagen peptide (mRCP) with two different ranges of interconnected pore sizes, Small-mRCP (S-mRCP) a range 100-300 μm and Large-mRCP (L-mRCP) 200-500 μm, to compare the effect size on bone regeneration in calvarial defect.Calvarial defects were created Sprague-Dawley rats through surgical procedure. The divided into 2 groups: S-mRCP implanted group L-mRCP group. newly formed volume mineral density (BMD) was evaluated by...
ABSTRACT Outbreaks caused by vancomycin-resistant enterococci that transcend jurisdictional boundaries are occurring worldwide. This study focused on a enterococcus outbreak occurred between 2018 and 2021 across two cities in Hiroshima, Japan. The involved genetic phylogenetic analyses using whole-genome sequencing of 103 isolates to identify the source transmission routes outbreak. Phylogenetic analysis was performed core genome multilocus sequence typing single-nucleotide polymorphisms;...
The incidence of streptococcal toxic shock syndrome (STSS) due to group B Streptococcus (GBS) has been increasing annually in Japan and is becoming a serious challenge. Furthermore, recent years, penicillin- or clindamycin-resistant strains used treating have reported. However, no report analyzed >100 isolates causing syndrome. Therefore, we aimed perform serotyping antimicrobial susceptibility testing 268 isolated from cases involving nonpregnant adult patients between 2014 2021. most...
Abstract Autografts and allografts are currently considered the gold standard for grafting surgery; however, to meet growing demand in fast-aging societies, synthetic biomaterials will play an increasingly important role. Here we report a biodegradable scaffold material composed of recombinant polypeptide based on human type I collagen alpha 1 chain (RCPhC1) as source hydrogel-based graft materials. The flexibility engineer ideal characteristics bone grafts was demonstrated. critical...
Abstract Platelet‐rich fibrin (PRF) is widely used in regenerative medicine. Nonetheless, major issues include its controversial effects on bone regeneration and a lack of quality‐assured glass tubes required for coagulation. We porous particles (FBG) comprising recombinant RGD motif‐enriched collagen I‐like protein to activate the coagulation pathway examined resulting PRF–FBG complex regeneration. Human whole‐blood samples were mixed with FBG plastic centrifuged prepare complex....
Microvascular changes during the development of follicles in bovine ovaries were studied by scanning electron microscopy corrosion casts. A clear vascular plexus ovarian appeared at stage when secondary 200-400 microns diameter. The consisted initially a thin, roughly structured and single-layered capillary network. During follicular development, microvascular architecture antral was arranged as two independent plexuses. inner plexus, which received spiral arteriole, dense sinusoidal network...
This study aimed to examine the bone-forming ability of medium-cross-linked recombinant collagen peptide (mRCP) particles developedbased on human type I, contains an arginyl-glycyl-aspartic acid-rich motif, fabricated as bone filling material, compared that autologous graft. Calvarial defects were created in immunodeficient rats though a surgical procedure. The divided into 2 groups: mRCP graft and tibia (bone graft). formation potential was evaluated by micro-computed tomography...
The present study demonstrated the microvasculature of bovine mature follicles and microvascular changes with ovulation. consisted 2 capillary plexuses, inner outer. plexus a thick, dense sinusoidal layer rough arterioles venules. Their was character-ized as an independent microcircular unit receiving only one afferent vessel. Microvascular ovulation appeared in plexus. In preovulatory follicles, capillaries gave rise to topographical increases permeability. These phenomena may play...
Bone graft materials provide a scaffold for migrating cells bone regeneration. One of the major challenges is to support adequate neovascularization in and tissue. Vascular endothelial have been shown recognize integrin-binding Arg-Gly-Asp (RGD) sequence natural extracellular matrix (ECM) molecules. Here, we report material composed an RGD-enriched recombinant polypeptide based on human type I collagen alpha 1 chain (RCPhC1) propose category called matrix. RCPhC1 demonstrated significantly...
We recently demonstrated the bone-forming potential of medium-cross-linked recombinant collagen peptide (mRCP) in animal models bone defects. However, these studies were limited to a 4-week observation period; therefore, present study, we aimed further evaluate mRCP as suitable graft material for alveolar cleft by analyzing its potential, osteogenic-inducing ability, and biodegradation over an extended period 12 weeks, using rat critical-size calvarial defect model.Using Sprague-Dawley rats,...
The Tsushima leopard cat (TLC) Prionailurus bengalensis euptilurus, a subspecies of P. bengalensis, is designated National Natural Monument Japan, and lives only on Island, Nagasaki Prefecture, Japan. TLCs are threatened by various infectious diseases. Feline leukemia virus (FeLV) causes serious disease with poor prognosis in cats. Therefore, the transmission FeLV from domestic cats (TDCs) to may threaten TLC population. We investigated infection status both TDCs Island screening blood...
Abstract Aim Recombinant bone matrix (RBM) is a newly conceived and engineered porous graft granule of average size 600 μm composed purified recombinant collagen peptide. We sought to examine the behaviour with time RBM that was grafted in canine tooth extraction socket. Materials Methods The socket hemisectioned mandibular third premolar distal root granules, whereas opposite side served as non‐grafted control. samples were harvested at 1, 3 6 months healing subjected micro‐CT imaging...
This study aimed to examine the optimal cross-link density of recombinant peptide (RCP) particles, based on human collagen type I, for bone reconstruction in alveolar cleft. Low- (group 1), medium- 2), and high- 3) cross-linked RCP particles were prepared by altering duration heat-dependent dehydration reaction. Rat palatine fissures (n = 45), analogous congenital defects, examined evaluate potential formation three different particles. Microcomputed tomography images obtained measure volume...
Feline leukemia virus (FeLV) induces neoplastic and nonneoplastic diseases in cats. The transduction of cellular genes by FeLV is sometimes observed associated with including lymphoma sarcoma. Here, we report the first natural case feline Notch2 an infected cat multicentric hypercalcemia. We cloned recombinant FeLVs harboring env gene. was able to activate expression a reporter gene, similar what previously reported cats experimental FeLV-induced thymic lymphoma. Our findings suggest that...
The microvasculature of bovine corpora lutea during pregnancy was studied by scanning electron microscopy using corrosion casts. Only one spiral arteriole branched directly from the ovarian artery entered blood vascular bed pregnant lutea. consisted numerous lobular sinusoidal capillary plexuses pregnancy, and were characteristic to a large corpus luteum. At late stage arteriovenous anastomoses situated within plexus composed thin capillaries. These may induce decrease in flow plexus,...
A Correction to this paper has been published: https://doi.org/10.1038/s43246-020-00111-0