A5061484680- Pituitary Gland Disorders and Treatments
- Stress Responses and Cortisol
- Growth Hormone and Insulin-like Growth Factors
- Adrenal Hormones and Disorders
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Cancer, Hypoxia, and Metabolism
- Estrogen and related hormone effects
- Chemokine receptors and signaling
- TGF-β signaling in diseases
- Reproductive System and Pregnancy
- Retinoids in leukemia and cellular processes
- Hormonal Regulation and Hypertension
- Ubiquitin and proteasome pathways
- Tryptophan and brain disorders
- Nuclear Receptors and Signaling
- NF-κB Signaling Pathways
- Neuropeptides and Animal Physiology
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Adenosine and Purinergic Signaling
- Glioma Diagnosis and Treatment
- interferon and immune responses
- Angiogenesis and VEGF in Cancer
- RNA modifications and cancer
Fundación Ciencias Exactas y Naturales
2016-2025
University of Buenos Aires
2016-2025
Biomedicine Research Institute of Buenos Aires - CONICET - Partner Institute of the Max Planck Society
2016-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2002-2022
Max Planck Institute of Psychiatry
2006-2017
Institut Pasteur de Montevideo
2015
Fundação Oswaldo Cruz
2015
Max Planck Society
2003-2012
Ghent University
2012
University of Stuttgart
2001-2004
Abstract Nur factors are critical for proopiomelanocortin (POMC) induction by CRH in corticotrophs, but the pathways linking to unknown. In this study we show that AtT-20 corticotrophs and cAMP induce Nur77 Nurr1 expression transcription at NurRE site protein kinase A (PKA) calcium-dependent -independent mechanisms. Calcium depend on calmodulin II (CAMKII) activity, calcium-independent accounted part MAPK activation (Rap1/B-Raf/MAPK-ERK kinase/ERK1/2), demonstrated use of molecular...
Abstract Senescent cells produce a Senescence‐Associated Secretory Phenotype (SASP) that involves factors with diverse and sometimes contradictory activities. One key SASP factor, interleukin‐6 (IL‐6), has the potential to amplify cellular senescence in SASP‐producing an autocrine action, while simultaneously inducing proliferation neighboring cells. The underlying mechanisms for contrasting actions remain unclear. We found action does not involve IL‐6 secretion nor interaction receptor...
Cushing syndrome is caused by an excess of adrenocorticotropic hormone (ACTH) production neuroendocrine tumors, which subsequently results in chronic glucocorticoid excess. We found that retinoic acid inhibits the transcriptional activity AP-1 and orphan receptors Nur77 Nurr1 ACTH-secreting tumor cells. Retinoic treatment resulted reduced pro-opiomelanocortin transcription ACTH production. inhibition was also observed human pituitary cells a small-cell lung cancer cell line, but not normal...
Pituitary tumor development involves clonal expansion stimulated by hormones and growth factors/cytokines. Using mRNA differential display, we found that the bone morphogenetic protein (BMP) inhibitor noggin is down-regulated in prolactinomas from dopamine D2-receptor-deficient mice. BMP-4 overexpressed taken female mice, but expression of highly homologous BMP-2 does not differ normal pituitary tissue prolactinomas. other prolactinoma models, including estradiol-induced rat human...
The pituitary gland plays a central role in the interactions between immune and neuroendocrine systems. expression of receptors for interleukin-1 (IL-1), IL-2, IL-6 intrinsic production these ILs by cells have been described. Previous studies focused on way cytokines influence hormone secretion. We determined whether, addition to effects, could affect cell proliferation. In GH3 cells, both IL-2 (1-100 U/ml) (10-500 significantly stimulated [3H]thymidine incorporation count. contrast,...
Serotonin and melatonin inhibit phytohemagglutinin- (PHA) induced interferon-gamma (IFN-gamma) production by lymphocytes. In this paper, it is shown that IFN-gamma-increased tryptophan uptake lymphocytes macrophages led to an enhanced of serotonin. When IFN-gamma serotonin were added together a lymphocyte culture, N-acetyl was increased, whereas the path 5-hydroxy-indoleacetic acid remained unchanged. Therefore, stimulated inhibition synthesis these indoleamines suggest hypothesis for...
We investigated whether non-abortive maternal infections would compromise fetal brain development and alter hypothalamic-pituitary-adrenocortical (HPA) axis functioning when adult. To study putative teratogenic effects of a T cell-mediated immune response versus an endotoxic challenge, 10-d-pregnant rats received single intraperitoneal injection 5 x 10(8) human red blood cells (HRBC) or gram-negative bacterial endotoxin (Escherichia coli LPS: 30 micrograms/kg). The adult male progeny (3 mo...
Glucocorticoids (GCs) immunosuppression acts via regulation of several transcription factors (TF), including activating protein (AP)-1, NF-B, and NFAT.GCs inhibit Th1 cytokines promote a shift toward Th2 differentiation.Th1 phenotype depends on TF T-bet.In this study, we examined GC T-bet.We found that GCs T-bet transcriptional activity.We show glucocorticoid receptor (GR) physically interacts with both in transfected cell lines primary splenocyte cultures endogenous GR T-bet.This...
Cushing's disease is almost always caused by an ACTH-secreting pituitary tumor, but effective medical therapy currently limited. Because retinoic acid has been shown to be potentially useful in decreasing corticotroph secretion and proliferation rodent models, we have studied its action dogs with disease. A randomized treatment (n = 22) vs. ketoconazole 20) was assigned for a period of 180 d. Clinical signs, plasma ACTH α-MSH, the cortisol/creatinine urine ratio, magnetic resonance imaging...
The purinergic P2X7 receptor (P2X7R) has attracted considerable interest as a potential target for various central nervous system (CNS) pathologies including affective and neurodegenerative disorders. To date, the distribution cellular localization of P2X7R in brain are not fully resolved matter debate mainly due to limitations existing tools. However, this knowledge should be prerequisite understanding contribution disease. Here, we generated genetic mouse model by humanizing mammalian...
The production of IL-1 and IL-6 by pituitary cells has recently been demonstrated. In this study we investigated the expression IL-2 its receptor (IL-2R) different species. Northern blots, a single hybridizing band 1 kb, identical to that in normal stimulated lymphocytes, was obtained with specific probes. mouse AT-20 tumor cell line, mRNA detected after stimulation corticotropin-releasing hormone or phorbol myristate acetate. human corticotrophic adenoma cells, basal as well secretion were...
Corticotropin-releasing hormone (CRH) coordinates hormonal and behavioral responses to stress. The mitogen-activated protein kinase extracellular signal-related 1/2 (ERK1/2) mediates several functions in different forebrain structures recently has been implicated CRH signaling cultured cells. To study vivo CRH-mediated activation of central ERK1/2, we investigated the expression pattern phosphorylated ERK1/2( p -ERK1/2) mouse brain after intracerebroventricular injections. As shown by...
Glucocorticoid (GC) immunosuppression and anti-inflammatory action involve the regulation of several transcription factors (TFs). GCs inhibit acute production T-helper (Th) 1 Th2 cytokines but ultimately favor a shift toward phenotype. transcriptional activity T-bet Th1 TF by transrepression mechanism. Here we analyze GC GATA-3, master driver differentiation. We found that inhibitGATA-3 activity. demonstrate this mechanism does not physical interaction between glucocorticoid receptor (GR)...
A single nucleotide polymorphism substitution from glutamine (Gln, Q) to arginine (Arg, R) at codon 460 of the purinergic P2X7 receptor (P2X7R) has repeatedly been associated with mood disorders. The P2X7R-Gln460Arg variant per se is not compromised in its function. However, heterologous expression together wild-type P2X7R recently demonstrated impair Here we show that this also applies humanized mice coexpressing both human variants. Primary hippocampal cells derived heterozygous showed an...
There is increasing evidence that hormones play an important role in the control of endothelial cell function and growth by regulating production vascular factor (VEGF). VEGF regulates permeability represents most powerful for cells. In normal anterior pituitary, has been detected only folliculostellate (FS) present study, regulation release from FS-like mouse TtT/GF cells, FS cells rat pituitary monolayer cultures was investigated using a specific ELISA. Basal demonstrated both...