A5001609613- Plant and Fungal Interactions Research
- Plant Virus Research Studies
- Plant Disease Management Techniques
- Plant Disease Resistance and Genetics
- Forest Insect Ecology and Management
- Bacteriophages and microbial interactions
- Vector-Borne Animal Diseases
Okayama University
2012-2025
Summary To reveal mycovirus diversity, we conducted a search of as‐yet‐unexplored Mediterranean isolates the phytopathogenic ascomycete Rosellinia necatrix for virus infections. Of seventy‐nine, eleven fungal tested RNA virus‐positive, with many showing coinfections, indicating incidence 14%, which is slightly lower than that (approximately 20%) previously reported extensive surveys over 1000 Japanese R. isolates. All viral sequences were fully or partially characterized by Sanger and...
ABSTRACT Capsidless yadokariviruses (members of the order Yadokarivirales ) with (+)RNA genomes divert capsid their partner icosahedral double-stranded RNA (dsRNA) viruses in different families Ghabrivirales into replication site. A yadokarivirus, AfSV2, has been reported from a German strain ascomycete fungus Aspergillus foetidus coinfected by two dsRNA viruses, victorivirus (AfSV1 , family Pseudototiviridae and an alternavirus (AfFV, Alternaviridae ). Here, we identified AfSV1 as AfSV2...
Previously, we have reported the ability of a symptomless hypovirus Cryphonectria 4 (CHV4) chestnut blight fungus to facilitate stable infection by co-infecting mycoreovirus 2 (MyRV2)—likely through inhibitory effect CHV4 on RNA silencing (Aulia et al., Virology, 2019). In this study, N-terminal portion polyprotein, termed p24, is identified as an autocatalytic protease capable suppressing host antiviral silencing. Using bacterial expression system, p24 shown cleave autocatalytically at...
Partitiviruses (dsRNA viruses, family Partitiviridae) are ubiquitously detected in plants and fungi. Although previous surveys suggested their omnipresence the white root rot fungus, Rosellinia necatrix, only a few of them have been molecularly biologically characterized thus far. We report characterization total 20 partitiviruses from 16 R. necatrix strains belonging to 15 new species, for which 11 25 were proposed, 5 previously reported species. The newly identified taxonomically placed...
Cryphonectria is a fungal genus associated with economically significant disease of trees. Herein we characterized novel double-stranded RNA virus from the species naterciae, unexplored as host. De novo assembly RNA-seq data and Sanger sequencing RACE (rapid amplification cDNA ends) clones gave complete, non-segmented genome (10,164 bp) termed naterciae fusagravirus (CnFGV1) that was phylogenetically placed within previously proposed viral family Fusagraviridae. Of 31 field-collected strains...
Viruses typically encode their own capsids that encase genomes. However, a capsidless positive-sense single stranded RNA [(+)ssRNA] virus, YkV1, depends on an unrelated double-stranded (dsRNA) YnV1, for encapsidation and replication.
We have previously discovered a virus neo-lifestyle exhibited by capsidless positive-sense (+), single-stranded (ss) RNA YkV1 (family Yadokariviridae) and an unrelated double-stranded (ds) YnV1 (proposed family "Yadonushiviridae") in phytopathogenic ascomycete, Rosellinia necatrix. has been proposed to replicate the capsid provided as if it were dsRNA enhance replication return. Recently, viruses related (yadokariviruses) isolated from diverse ascomycetous fungi. However, remains obscure...
A novel dsRNA virus (Cryphonectria carpinicola fusagravirus 1, CcFGV1), isolated from a Japanese strain (JS13) of Cryphonectria carpinicola, was thoroughly characterized. The biological comparison set isogenic CcFGV1-infected and -free (JS13VF) strains indicated asymptomatic infection by CcFGV1. sequence analysis showed that the has two open reading frame (ORF) genome 9.6 kbp with RNA-directed RNA polymerase domain encoded ORF2. N-terminal sequencing peptide mass fingerprinting an...
Cryphonectria is a fungal genus associated with economically significant disease of trees. Herein we characterized novel double-stranded RNA virus from the species naterciae, unexplored as host. De novo assembly RNA-seq data and Sanger sequencing RACE (rapid amplification cDNA ends) clones gave complete, non-segmented genome (10,164 bp) termed naterciae fusagravirus (CnFGV1) that was phylogenetically placed within previously proposed viral family Fusagraviridae. Of 31 field-collected strains...
The yadokari/yadonushi nature is a recently discovered virus lifestyle; "yadokari" refers to the ability of capsidless positive-sense (+) RNA viruses (yadokariviruses) utilize capsids phylogenetically distant double-stranded (dsRNA) possibly as replication site, while "yadonushi" dsRNA provide yadokariviruses. This virus-virus interaction, however, has been only studied with limited pathosystems. Here, we established new study model (+)RNA yadokarivirus YkV3 (family Yadokariviridae) and its...