A5000040206- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Viral gastroenteritis research and epidemiology
- Virology and Viral Diseases
- Virus-based gene therapy research
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Enzyme Structure and Function
- Animal Virus Infections Studies
- Mosquito-borne diseases and control
- Advanced Electron Microscopy Techniques and Applications
- Respiratory viral infections research
- Genomics and Phylogenetic Studies
- Insect symbiosis and bacterial influences
- Bacterial Genetics and Biotechnology
- Viral Infections and Outbreaks Research
- Glycosylation and Glycoproteins Research
- Enzyme Production and Characterization
- Monoclonal and Polyclonal Antibodies Research
- Animal Disease Management and Epidemiology
- RNA modifications and cancer
- Parvovirus B19 Infection Studies
Centro Nacional de Biotecnología
2016-2025
Madrid Institute for Advanced Studies
2018-2024
Consejo Superior de Investigaciones Científicas
1994-2022
Leiden University
2011
Universidad Autónoma de Madrid
1995-2009
Icahn School of Medicine at Mount Sinai
2009
National Institutes of Health
1994-1999
National Institute of Arthritis and Musculoskeletal and Skin Diseases
1994-1999
Center for Information Technology
1999
Brookhaven National Laboratory
1997
The packaging of proteins into discrete compartments is an essential feature for cellular efficiency. Inspired by Nature, we harness virus-like assemblies as artificial nanocompartments enzyme-catalyzed cascade reactions. Using the negative charges nucleic acid tags, develop a versatile strategy to promote efficient noncovalent co-encapsulation enzymes within single protein cage cowpea chlorotic mottle virus (CCMV) at neutral pH. encapsulation results in stable 21–22 nm sized CCMV-like...
Upon attachment to their respective receptor, human rhinoviruses (HRVs) are internalized into the host cell via different pathways but undergo similar structural changes. This ultimately results in delivery of viral RNA cytoplasm for replication. To improve our understanding conformational modifications associated with release genome, we have determined X-ray structure at 3.0 Å resolution end-stage HRV2 uncoating, empty capsid. The shows important changes capsid protomer. In particular, a...
Viruses are a paradigm of the economy genome resources, reflected in their multiplication strategy and for own structure. Although there is enormous structural diversity, viral always enclosed within proteinaceous coat, most virus species haploid; only exception to this rule highly pleomorphic enveloped viruses. We performed an in-depth characterization infectious bursal disease (IBDV), non-enveloped icosahedral dsRNA with bisegmented genome. Up 6 natural populations can be purified, which...
Recent years have witnessed the emergence of bacterial semiorganelle encapsulins as promising platforms for bio-nanotechnology. To advance development nanoplatforms, a functional and structural basis these assemblies is required. Encapsulin from Brevibacterium linens known to be protein-based vessel an enzyme cargo in its cavity, which could replaced with foreign cargo, resulting modified encapsulin. Here, we characterize native structure B. both using cryo-electron microscopy (cryo-EM)....
Most bacterial viruses need a specialized machinery, called "tail," to inject their genomes inside the cytoplasm without disrupting cellular integrity. Bacteriophage T7 is well characterized member of Podoviridae family infecting Escherichia coli, and it has short noncontractile tail that assembles sequentially on viral head after DNA packaging. The complex around 2.7 MDa composed at least four proteins as follows: connector (gene product 8, gp8), tubular gp11 gp12, fibers (gp17). Using...
Phthalocyanines (Pc) are non-natural organic dyes with wide and deep impact in materials science, based on their intense absorption at the near-infrared (NIR), long-lived fluorescence high singlet oxygen ((1)O2) quantum yields. However, Pcs tend to stack buffer solutions, losing ability generate oxygen, which limits scope of application. Furthermore, challenging organize crystalline structures. Protein cages, other hand, very promising biological building blocks that can be used different...
Members of the family Chrysoviridae are isometric, non-enveloped viruses with segmented, linear, dsRNA genomes. There 3–7 genomic segments, each which is individually encapsidated. Chrysoviruses infect fungi, plants and possibly insects, may cause hypovirulence in their fungal hosts. have no known vectors lack an extracellular phase to replication cycle; they transmitted via intracellular routes within individual during hyphal growth, asexual or sexual spores, between individuals...
A cDNA corresponding to the coding region of VP1, putative RNA-dependent RNA polymerase, infectious bursal disease virus (IBDV) was cloned and inserted into genome a vaccinia inducible expression vector. The molecular mass antigenic reactivity VP1 expressed in mammalian cells are identical those its counterpart IBDV-infected cells. results presented here demonstrate that is efficiently incorporated IBDV virus-like particles (VLPs) produced coexpressing polyprotein VP1. Incorporation VLPs...
The genomes of double-stranded (ds)RNA viruses are never exposed to the cytoplasm but confined and replicated from a specialized protein-bound compartment—the viral capsid. We have used cryoelectron microscopy three-dimensional image reconstruction study this compartment in case L-A, yeast virus whose capsid consists 60 asymmetric dimers Gag protein (76 kD). At 16-Å resolution, we distinguish multiple domains elongated subunits, nonequivalent packing is reflected subtly different...
Infectious bursal disease virus (IBDV), a member of the Birnaviridae family, is double-stranded RNA virus. The IBDV capsid formed by two major structural proteins, VP2 and VP3, which assemble to form T=13 markedly nonspherical capsid. During viral infection, initially synthesized as precursor, called VPX, whose C end proteolytically processed mature during assembly. We have computed three-dimensional maps virus-like particles built up alone using electron cryomicroscopy image-processing...
Significance Human rhinoviruses (HRVs) cause the common cold and exacerbate chronic pulmonary diseases. Their single-stranded RNA genome is protected by an icosahedral capsid must be released into host cell cytosol for translation replication. Using X-ray cryo-EM analyses, we identified structural alterations that take place in virus architecture during infection. In acidic endosomes vivo our experimental conditions, native virion converted expanded, porous uncoating intermediate A-particle....
In 2010 a new Lagovirus related to rabbit haemorrhagic disease virus (RHDV) emerged in France and has since rapidly spread throughout domestic wild populations of several European countries. The virus, termed RHDV2, exhibits distinctive genetic, antigenic pathogenic features. Notably, RHDV2 kills rabbits previously vaccinated with RHDV vaccines. Here we report for the first time generation characterization RHDV2-specific virus-like particles (VLPs). Our results further confirmed differential...
Nucleic acids are the natural cargo of viruses and key determinants that affect viral shell stability. In some cases genome structurally reinforces shell, whereas in others packaging causes internal pressure can induce destabilization. Although it is possible to pack heterologous cargoes inside virus-derived shells, little known about physical these artificial nanocontainers' Atomic force three-dimensional cryo-electron microscopy provided mechanical structural information mechanisms cage...
Phthalocyanines (Pc) are dyes in widespread use materials science and nanotechnology, with numerous applications medicine, photonics, electronics energy conversion. With the aim to construct biohybrid materials, we here prepared analyzed structure of two Pc-loaded virus-like particles (VLP) diameters 20 28 nm (i.e., T = 1 3 icosahedral symmetries, respectively). Our cryo-electron microscopy (cryo-EM) studies show an unprecedented, very high level Pc molecule organization within both VLP. We...
Bacteriophage T5, a Siphovirus belonging to the order Caudovirales, has flexible, three-fold symmetric tail, which three L-shaped fibres are attached. These recognize oligo-mannose units on bacterial cell surface prior infection and composed of homotrimers pb1 protein. Pb1 1396 amino acids, carboxy-terminal 133 residues form trimeric intra-molecular chaperone that is auto-proteolyzed after correct folding. The structure trimer 970–1263 was determined by single anomalous dispersion phasing...
Birnaviruses are a group of double-stranded RNA (dsRNA) viruses infecting birds, fish and insects. Early endosomes (EE) constitute the platform for viral replication. Here, we study mechanism birnaviral targeting EE membranes. Using Infectious Bursal Disease Virus (IBDV) as model, validate that protein 3 (VP3) binds to phosphatidylinositol-3-phosphate (PI3P) present in We identify domain VP3 involved PI3P-binding, named P2 localized core VP3, establish critical role arginine at position 200...
ABSTRACT Capsidless yadokariviruses (members of the order Yadokarivirales ) with (+)RNA genomes divert capsid their partner icosahedral double-stranded RNA (dsRNA) viruses in different families Ghabrivirales into replication site. A yadokarivirus, AfSV2, has been reported from a German strain ascomycete fungus Aspergillus foetidus coinfected by two dsRNA viruses, victorivirus (AfSV1 , family Pseudototiviridae and an alternavirus (AfFV, Alternaviridae ). Here, we identified AfSV1 as AfSV2...