A5004345445- Plant and Fungal Interactions Research
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Plant Disease Management Techniques
- RNA Research and Splicing
- RNA modifications and cancer
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Forest Insect Ecology and Management
- Insect-Plant Interactions and Control
- Bacterial Genetics and Biotechnology
- Hepatitis C virus research
- Entomopathogenic Microorganisms in Pest Control
- Plant-Microbe Interactions and Immunity
- Viral Infections and Immunology Research
- Computational Drug Discovery Methods
- Viral Infections and Vectors
- Plant Pathogens and Fungal Diseases
- Wastewater Treatment and Nitrogen Removal
- Gene Regulatory Network Analysis
- Protein Structure and Dynamics
- Antibiotic Resistance in Bacteria
- Viral Infectious Diseases and Gene Expression in Insects
Imperial College London
2016-2025
University of Hertfordshire
2018-2025
Pasteur Hellenic Institute
2012-2020
The London College
2019
National and Kapodistrian University of Athens
2012-2017
Forthnet (Greece)
2013
Institut Pasteur
2013
Abstract Non-self recognition is a fundamental aspect of life, serving as crucial mechanism for mitigating proliferation molecular parasites within fungal populations. However, studies investigating the potential interference plants with non-self mechanisms are limited. Here, we demonstrate pronounced increase in efficiency horizontal mycovirus transmission between vegetatively incompatible Sclerotinia sclerotiorum strains planta compared to vitro. This increased associated elevated proline...
The entomopathogenic fungus Beauveria bassiana has a wide host range and is used as biocontrol agent against arthropod pests. Mycoviruses have been described in phytopathogenic fungi while their presence reported only rarely. Here we show that 21.3% of collection B. isolates sourced from worldwide locations, harbor dsRNA elements. Molecular characterization these elements revealed the prevalence mycoviruses belonging to Partitiviridae Totiviridae families, smallest virus date, family...
Significance Mycoviruses generally contain dsRNA genomes but ssRNA and ssDNA examples are known. Mycovirus diversity is increasing, here we describe a unique example that contains four elements nominated Aspergillus fumigatus tetramycovirus-1 (AfuTmV-1). We show for the first time (to our knowledge) both purified AfuTmV-1 its infectious protoplasts virus genome not conventionally encapsidated has organization. Separation of genes encoding RNA-dependent RNA polymerase enzyme responsible...
Mycoviruses are widely present in all major groups of fungi but those entomopathogenic Metarhizium spp. remain understudied. In this investigation, a novel double-stranded (ds) RNA virus is isolated from majus and named partitivirus 1 (MmPV1). The complete genome sequence MmPV1 comprises two monocistronic dsRNA segments (dsRNA 2), which encode an RNA-dependent polymerase (RdRp) capsid protein (CP), respectively. classified as new member the genus Gammapartitivirus family Partitiviridae based...
Circular single-stranded DNA (ssDNA) viruses have been rarely found in fungi, and the evolutionary ecological relationships among ssDNA infecting fungi other organisms remain unclear. In this study, a novel circular virus, tentatively named Diaporthe sojae virus 1 (DsCDV1), was identified phytopathogenic fungus isolated from pear trees. DsCDV1 has monopartite genome (3185 nt size) encapsidated isometric virions (21-26 nm diameter). The comprises seven putative open reading frames encoding...
Members of the family Chrysoviridae are isometric, non-enveloped viruses with segmented, linear, dsRNA genomes. There 3–7 genomic segments, each which is individually encapsidated. Chrysoviruses infect fungi, plants and possibly insects, may cause hypovirulence in their fungal hosts. have no known vectors lack an extracellular phase to replication cycle; they transmitted via intracellular routes within individual during hyphal growth, asexual or sexual spores, between individuals...
Polymycoviridae is a growing family of mycoviruses whose members typically have non-conventional capsids and multi-segmented, double-stranded (ds) RNA genomes. Beauveria bassiana polymycovirus (BbPmV) 1 known to enhance the growth virulence its fungal host, entomopathogenic ascomycete popular biological control agent B. . Here we report complete sequence BbPmV-3, which has six genomic dsRNA segments. Phylogenetic analysis RNA-dependent polymerase (RdRp) protein sequences revealed that...
Historically, the lacZ gene is one of most universally used reporters expression in molecular biology. Its activity can be quantified using an artificial substrate, o-nitrophenyl-ß-d-galactopyranoside (ONPG). However, traditional method for measuring LacZ (first described by J. H. Miller 1972) challenging a large number samples, prone to variability, and involves hazardous compounds lysis (e.g., chloroform, toluene). Here we describe single-step assay 96-well microplate reader with proven...
Members of the family Polymycoviridae are small viruses with multi-segmented and non-conventionally encapsidated double-stranded (ds) RNA genomes. Typically, polymycoviruses have four genomic segments, although some up to eight. The genus Polymycovirus includes several species whose members infect fungi (ascomycetes basidiomycetes), oomycetes, altering host morphology, sporulation, growth virulence. This is a summary International Committee on Taxonomy Viruses (ICTV) Report Polymycoviridae,...
To date, viroids have been found to naturally infect only plants, resulting in substantial losses for some crops. Whether or viroid-like RNAs non-plant hosts remains unknown. Here the existence of a set exogenous, single-stranded circular RNAs, ranging size from 157 450 nucleotides, isolated fungus Botryosphaeria dothidea and nominated B. (BdcRNAs) is reported. BdcRNAs replicate autonomously nucleus via rolling-circle mechanism following symmetric pathway. BdcRNA infection induces symptoms,...
Mycoviral infection can either be asymptomatic or have marked effects on fungal hosts, influencing them positively negatively. To fully understand the of mycovirus host, transcriptomic profiling four Beauveria bassiana isolates, including EABb 92/11-Dm that harbors mycoviruses, was performed 48 h following Tenebrio molitor via topical application injection. Genes participate in carbohydrate assimilation and transportation, those essential for survival oxidative stress tolerance, calcium...
Leptosphaeria biglobosa is a phytopathogenic ascomycete of Brassica napus that causes phoma stem canker/blackleg. A new double-stranded RNA (dsRNA) mycovirus from this fungus has been fully characterized. The virus genome five dsRNA segments, ranging in length 1,180 bp to 2,402 bp. Each single open reading frame flanked by 5' and 3' untranslated regions. proteins encoded dsRNAs 1 3, an RNA-dependent polymerase (RdRP) methyltransferase, respectively, have significant similarity those...
Low oxygen tension exerts a significant effect on the replication of several DNA and RNA viruses in cultured cells. In vitro propagation hepatitis C virus (HCV) has thus far been studied under atmospheric levels despite fact that liver tissue microenvironment is hypoxic. this study, we investigated efficiency HCV production actively dividing or differentiating human hepatoma cells low tensions. By using both replicons infection-based assays, was found to enhance whereas entry translation...
Phoma stem canker (blackleg) is one of the most important diseases winter oilseed rape (Brassica napus) worldwide and caused by a complex that comprises at least two species: Leptosphaeria maculans L. biglobosa. Screening panel field isolates from B. napus for presence mycoviruses revealed novel double-stranded RNA quadrivirus in biglobosa no viruses maculans. Following elimination mycovirus, virus-infected virus-free isogenic lines were created. A direct comparison growth virulence these...
Abstract Transcription is fundamentally noisy, leading to significant heterogeneity across bacterial populations. Noise often attributed burstiness, but the underlying mechanisms and their dependence on mode of promotor regulation remain unclear. Here, we measure E. coli single cell mRNA levels for two stress responses that depend sigma factors with different transcription initiation (σ 70 σ 54 ). By fitting a stochastic model observed distributions, show transition from low high expression...