A5026722006- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Antibiotic Resistance in Bacteria
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Cancer therapeutics and mechanisms
- Viral Infections and Immunology Research
- RNA modifications and cancer
- Signaling Pathways in Disease
- Escherichia coli research studies
- Click Chemistry and Applications
- Gene Regulatory Network Analysis
- Clostridium difficile and Clostridium perfringens research
- Chemical Synthesis and Analysis
- Tuberculosis Research and Epidemiology
- Enzyme Structure and Function
- Legume Nitrogen Fixing Symbiosis
- RNA Interference and Gene Delivery
- Neutropenia and Cancer Infections
- Microbial Natural Products and Biosynthesis
University of Belgrade
2024
Imperial College London
2009-2022
The London College
2019
Institute of Molecular Genetics and Genetic Engineering
2003-2005
Institute of Molecular Genetics
1999
The bacterial AAA+ enhancer-binding proteins (EBPs) HrpR and HrpS (HrpRS) of Pseudomonas syringae (Ps) activate σ54-dependent transcription at the hrpL promoter; triggering type-three secretion system-mediated pathogenicity. In contrast with singly acting EBPs, evolution strictly co-operative HrpRS pair raises questions potential benefits mechanistic differences this control system offers. Here, we show distinct properties variants, indicating functional specialization these non-redundant,...
The type III secretion system (T3SS) is a principal virulence determinant of the model bacterial plant pathogen Pseudomonas syringae T3SS effector proteins inhibit defense signaling pathways in susceptible hosts and elicit evolved immunity resistant plants. extracytoplasmic function sigma factor HrpL coordinates expression most genes. Transcription hrpL dependent on sigma-54 codependent enhancer binding HrpR HrpS for promoter activation. oriented adjacently to divergently from HrpL-dependent...
σ54-dependent transcription requires activation by bacterial enhancer binding proteins (bEBPs). bEBPs are members of the AAA+ (ATPases associated with various cellular activities) protein family and typically form hexameric structures that crucial for their ATPase activity. The precise mechanism which energy derived from ATP hydrolysis is coupled to biological output has several unknowns. Here we use Escherichia coli PspF, a model bEBP involved in stress response genes (psp operon), study...
Transcription activator RamA is linked to multidrug resistance of Klebsiella pneumoniae through controlling genes that encode efflux pumps (acrA) and porin-regulating antisense RNA (micF). In bacteria, σ70 , together with activators, controls the majority by recruiting polymerase (RNAP) promoter regions. RNAP form a holoenzyme recognizes -35 -10 DNA consensus sites. Many activators bind upstream from can be broadly divided into two classes. acts as class I on acrA II micF, respectively. The...
Bacteriophage T7 infects Escherichia coli and evades the host restriction/modification system. The Ocr protein of was shown to exist as a dimer mimicking DNA bind restriction enzymes, thus preventing degradation viral genome by host. Here we report that can also inhibit transcription directly binding bacterial RNA polymerase (RNAP) competing with recruitment RNAP sigma factors. Using cryo electron microscopy, determined structures bound RNAP. show an binds in cleft, where key regions resides...
CRISPR-associated Rossmann fold (CARF) domain signaling underpins modulation of CRISPR-Cas nucleases; however, the RtcR CARF controls expression two conserved RNA repair enzymes, cyclase RtcA and ligase RtcB. Here, we demonstrate that RtcAB are required for RtcR-dependent transcription activation directly bind to CARF. catalytic activity is not complex formation with CARF, but essential yet sufficient RtcRAB-dependent activation, implying need an additional repair-dependent activating...
In this work, we describe an improved series of N-phenylpyrrolamide inhibitors that exhibit potent activity against DNA gyrase and are highly effective high-priority gram-positive bacteria. The most compounds show low nanomolar IC50 values Escherichia coli gyrase, in addition, compound 7c also inhibits E. topoisomerase IV the concentration range, making it a promising candidate for development dual these enzymes. All tested high selectivity towards human isoform IIα. Compounds 6a, 6d, 6e 6f...
The cysB gene product is a LysR-type regulatory protein required for expression of the cys regulon. mutants Escherichia coli and Salmonella, along with being auxotrophs cysteine, exhibit increased resistance to antibiotics novobiocin (Nov) mecillinam. In this work, by using λplacMu9 insertions creating random lacZ fusions, we identify gene, hslJ, whose appeared be in needed Nov resistance. Measurements hslJ::lacZ fusion demonstrated that hslJ negatively regulated CysB. addition observe...
The LysR-type transcriptional regulator (LTTR) CysB is a transcription factor in Escherichia coli cells, where as homotetramer it binds the target promoter regions and activates genes involved sulphur utilization sulphonate-sulphur metabolism, while negatively autoregulating its own transcription. hslJ gene was found to be regulated by directly correlated with novobiocin resistance of bacterium. cysB mutants showed upregulation : lacZ fusion exhibited increased resistance. In this study...
ABSTRACT In previous studies we demonstrated that mutations in the genes cysB , cysE and cls ( nov ) affect resistance of Escherichia coli to novobiocin (J. Rakonjac, M. Milic, D. J. Savic, Mol. Gen. Genet. 228:307–311, 1991; R. Ivanisevic, Ajdic, Bacteriol. 177:1766–1771, 1995). this work expand list with rpoN (the gene for RNA polymerase subunit ς 54 tRNA synthetase alaS argS ileS leuS . Similarly penicillin antibiotic mecillinam, mutants appears depend upon RelA-mediated stringent...
The plant pathogen Pseudomonas syringae pv. tomato DC3000 uses a type III secretion system (T3SS) to transfer effector proteins into the host. expression of T3SS is controlled by HrpL σ factor. Transcription hrpL 54 -dependent and bacterial enhancer-binding HrpR HrpS coactivate promoter. HrpV protein imposes negative control upon through direct interaction with HrpS. HrpG interacts relieves such control. sequence alignments across Hrp group I-type pathogens revealed conserved amino acids. To...
The KgmB methylase from Streptomyces tenebrarius was expressed and purified using the QIAexpress System. Two expression vectors were made: pQEK-N, which places a (His)6 tag at N-terminus, pQEK-C, C-terminus of recombinant protein. Kanamycin resistance E. coli cells containing either pQEK-N or pQEK-C plasmids confirmed functionality both KgmB-His fusion proteins in vivo. Interestingly, different levels observed between these two proteins. Namely, with N-terminus showed higher level...
Abstract Bacteriophage T7 infects Escherichia coli and evades the host defence system. The Ocr protein of was shown to exist as a dimer mimicking DNA bind restriction enzymes, thus preventing degradation viral genome by host. Here we report that can also inhibit transcription directly binding bacterial RNA polymerase (RNAP) competing with recruitment RNAP sigma factors. Using cryo electron microscopy, determined structures bound RNAP. show an binds in cleft, where key regions resides during...
CRISPR-associated Rossmann fold (CARF) domain signalling underpins the modulation of CRISPR-Cas systems. Unlike majority known CARF domains associated with nuclease activity in systems, transcriptional regulator RtcR modulates opposite function by activating an RNA end sealing system. The Rtc repair system Escherichia coli consists ofthe universally conserved cyclase RtcA and ligase RtcB, is to be induced antibiotics oxidative stress. We aim investigate mediated regulation vivo vitro . A...