A5065329168- Trace Elements in Health
- RNA and protein synthesis mechanisms
- Crystallization and Solubility Studies
- Enzyme Structure and Function
- X-ray Diffraction in Crystallography
- RNA Research and Splicing
- Boron Compounds in Chemistry
- Redox biology and oxidative stress
- Sulfur Compounds in Biology
- Renin-Angiotensin System Studies
- Advanced X-ray Imaging Techniques
- Nuclear Physics and Applications
- Radiopharmaceutical Chemistry and Applications
- Machine Learning in Materials Science
- Boron and Carbon Nanomaterials Research
- RNA modifications and cancer
- Erythrocyte Function and Pathophysiology
- Privacy, Security, and Data Protection
- Venomous Animal Envenomation and Studies
- Antimicrobial agents and applications
- Reproductive System and Pregnancy
- User Authentication and Security Systems
- Phagocytosis and Immune Regulation
- Advanced Clustering Algorithms Research
- Robotic Path Planning Algorithms
Michigan State University
2022-2025
University of Maryland, Baltimore
2017-2023
Korea University
2014-2019
University of Baltimore
2019
Georgia Institute of Technology
2014
ABSTRACT Synthetic metal chelators are widely used in industrial, clinical, and agricultural settings, leading to their accumulation the environment. We measured growth of Caulobacter crescentus , a soil aquatic bacterium, presence ubiquitous chelator ethylenediaminetetraacetic acid (EDTA) found that it restricts by lowering intracellular iron levels. Using barcoded transposon sequencing, we identified an operonic gene pair, cciT–cciO is required maintain homeostasis laboratory media during...
H2 S is a gaseous signaling molecule that modifies cysteine residues in proteins to form persulfides (P-SSH). One family of modified by are zinc finger (ZF) proteins, which contain multiple zinc-coordinating residues. Herein, we report the reactivity with ZF protein called tristetraprolin (TTP). Rapid persulfidation leading complete thiol oxidation TTP mediated was observed low-temperature ESI-MS and fluorescence spectroscopy. Persulfidation required O2 , reacts superoxide, as detected...
Pathogenesis of COVID-19 by SARS-CoV-2 resulted in a global pandemic and public health emergency 2020. Viral infection can induce oxidative stress through reactive oxygen species (ROS). Inflammation environmental are major sources after infection. Micronutrients such as iron, copper, zinc, manganese play various roles human tissues their imbalance blood impact immune responses against pathogens including SARS CoV-2. We hypothesized that alteration free metal ions during metal-catalyzed...
Tristetraprolin (TTP) is a nonclassical zinc finger (ZF) protein that plays key role in regulating inflammatory response. TTP regulates cytokines at the mRNA level by binding to AU-rich sequences present 3'-untranslated region, forming complex then degraded. contains two conserved CCCH domains with sequence CysX8CysX5CysX3His are activated bind RNA when coordinated. During inflammation, copper levels elevated, which associated increased A potential target for Cu(I) during inflammation TTP....
Combating influenza is one of the perennial global public health issues to be managed. Antiviral drugs are useful for treatment in absence an appropriate vaccine. However, appearance resistant strains necessitates a constant search new drugs. In this study, we investigated novel anti-influenza drug candidates using vitro and vivo assays. We identified hit compounds high-throughput screening method with green fluorescent protein-tagged recombinant virus. Through subsequent analyses their...
Zinc fluctuations regulate key steps in late oocyte and preimplantation embryo development; however, roles for zinc preceding stages early ovarian follicle development, when cooperative interactions exist between the somatic cells, are unknown. To understand of during we applied single cell X-ray fluorescence microscopy, a radioactive tracer, labile probe to measure individual mouse oocytes associated cells within follicles. Here, report significant stage-specific increase compartmental...
ZF proteins are ubiquitous eukaryotic that play important roles in gene regulation. ZFs contain small domains made up of a combination four cysteine and histidine residues, classified based on the identity these residues their spacing. One emerging class Cys3His (or CCCH) ZFs. These key regulating RNA. In this minireview, an overview CCCH ZFs, with focus tristetraprolin (TTP) is provided. TTP regulates inflammation by controlling cytokine mRNAs, there interest modulating activity to control...
Closo-carborane has been considered as an efficient boron-carrier for boron neutron capture therapy (BNCT) and attractive surrogate of lipophilic phenyl or cyclohexyl ring in drug design. Despite a great number carborane-containing ligands have synthesized evaluated, molecular modeling studies carborane with macromolecules rarely reported. We herein describe facile docking scoring-function strategy 16 estrogen receptor by using the commercial Gaussian, Chem3D Pro Discovery Studio (DS)...
The zinc finger protein tristetraprolin (TTP) regulates inflammation by downregulating cytokine mRNAs. Misregulation results in arthritis, sepsis and cancer, there is an interest modulating TTP activity with exogenous agents. Gold has anti-inflammatory properties recently been shown to modulate the signaling pathway that produces TTP, suggesting may be a target of gold. reactivity [AuIII (terpy)Cl]Cl2 was investigated UV/Vis spectroscopy, spin-filter inductively coupled plasma mass...
Abstract H 2 S is a gaseous signaling molecule that modifies cysteine residues in proteins to form persulfides (P‐SSH). One family of modified by are zinc finger (ZF) proteins, which contain multiple zinc‐coordinating residues. Herein, we report the reactivity with ZF protein called tristetraprolin (TTP). Rapid persulfidation leading complete thiol oxidation TTP mediated was observed low‐temperature ESI‐MS and fluorescence spectroscopy. Persulfidation required O , reacts superoxide, as...
Abstract Identification of potent agonists odorant receptors (ORs), a major class G protein‐coupled receptors, remains challenging due to complex receptor–ligand interactions. ORs are present in both olfactory and non‐chemosensory tissues, indicating roles beyond odor detection that may include modulating physiological functions non‐olfactory tissues. Selective specific for particular can be used investigate In this study, we designed synthesized novel synthetic dehydroacetic acid analogues...
The microscopy research at the Bionanoprobe (currently beamline 9-ID and later 2-ID after APS-U) of Argonne National Laboratory focuses on applying synchrotron X-ray fluorescence (XRF) techniques to obtain trace elemental mappings cryogenic biological samples gain insights about their role in critical activities. morphological aspects samples, this instance, bacterium Escherichia coli ( E. Coli ), also serve as label-free fingerprints identify cells that have been treated differently. key...
Synthetic metal chelators are widely used in industrial, clinical, and agricultural settings, leading to their accumulation the environment. We measured growth of Caulobacter crescentus , a soil aquatic bacterium, presence ubiquitous chelator ethylenediaminetetraacetic acid (EDTA) found that it restricts by lowering intracellular iron levels. Using barcoded transposon sequencing, we identified an operonic gene pair, cciT-cciO is required maintain homeostasis laboratory media during EDTA...