A5103275375- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Mass Spectrometry Techniques and Applications
- Solar and Space Plasma Dynamics
- Ionosphere and magnetosphere dynamics
- Chromatography in Natural Products
- Geophysics and Gravity Measurements
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Proteomics Techniques and Applications
- Analytical Methods in Pharmaceuticals
- Advanced Chemical Sensor Technologies
- Anesthesia and Neurotoxicity Research
- Protein purification and stability
- Biosensors and Analytical Detection
- Pharmacological Effects of Natural Compounds
Environmental Molecular Sciences Laboratory
2023
Pacific Northwest National Laboratory
2023
University of Oklahoma
2018-2021
Institute of Forensic Science
2019-2020
In many areas of application, key objectives chemical separation and analysis are to minimize the sample quantity while maximizing information obtained. Increasing measurement sensitivity is especially critical for proteomics research, when processing trace samples where multiple measurements desired. A rich collection technologies has been developed, but resulting remains insufficient achieving in-depth coverage proteomic as small single cells. Here, we combine picoliter-scale liquid...
Separation speed and resolution are two important figures of merit in chromatography. Often, one gains the at cost resolution, vice versa. Scientists have employed short-packed columns for ultrafast separations but encountered challenges such as limited mobile phase velocity, extra-column effect caused band broadening, column packing difficulty. We recently demonstrated ultrahigh resolutions narrow open tubular liquid chromatography (NOTLC); this allows us to trade some speed. In work, we...
We report that we can achieve extremely high separation efficiencies using a narrow, open, tubular (NOT) column for liquid-chromatographic separations, and carry out these separations under an elution pressure of no more than 50 bar. To improve the efficiency in packed-column liquid chromatography, one most effective approaches is to reduce monodispersed-particle sizes. A direct consequence reduced particle size increased pressure. High have been obtained ultrahigh-performance chromatography...
We have experimentally demonstrated the extraordinarily high resolving power of liquid chromatography (LC) using a narrow open tubular (OT) column. In this work, we show that can further increase its efficiency, peak capacity, and separation speed by elevating operation (or column) temperature; all these three numbers be improved without mutual compromises. use mixture five amino acids as sample efficiency 34%–260% speeds 7%–10% raising temperature from 30 to 70 °C. When 2 μm i.d. × 80 cm in...
Multiplexed molecular profiling of tissue microenvironments, or spatial omics, can provide critical insights into cellular functions and disease pathology. The coupling laser microdissection with mass spectrometry-based proteomics has enabled deep unbiased mapping >1000 proteins. However, the throughput is often limited due to tedious two-step procedures, sequential cutting, sample collection. procedure also hinders further improvement resolution <10 μm as needed for subcellular proteomics....
The advancements in life science research mandate effective tools capable of analyzing large numbers samples with low quantities and high complexities. As an essential analytical tool for this research, liquid chromatography (LC) encounters ever-increasing demand enhanced resolving power, accelerated analysis speed, reduced limit detection. Although theoretical studies have indicated that open tubular (OT) columns can produce superior power under comparable elution pressures times,...
Objective To establish an ultra-high performance liquid chromatography-tandem mass spectrometry （UPLC-MS/MS） rapid determination method for simultaneous analysis of 20 fentanyl-related substances in blood. Methods With fentanyl-D5 as internal standard, the blood was extracted by liquid-liquid extraction （LLE）, then separated with ACQUITY UPLC HSS T3 chromatographic column, and finally were simultaneously analyzed multiple reaction monitoring （MRM） mode. Results The limits detection （LOD） all...
Objective To study the influence of halogenated hydroxyl-alkanes inhalation anesthetic on determination ethanol content in blood. Methods Halogenated were analyzed by headspace gas chromatography with double column confirmatory detection method. The blood sample was explored under different conditions KB-BAC1/ KB-BAC2 and J&W DB-ALC1/DB-ALC2 chromatographic column. Results retention time sevoflurane enflurane similar to that tert butanol respectively when using column, interfered blood; only...