- Microbial Fuel Cells and Bioremediation
- Electrochemical sensors and biosensors
- Metal Extraction and Bioleaching
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Membrane-based Ion Separation Techniques
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Electrochemical Analysis and Applications
- Chromium effects and bioremediation
- Arsenic contamination and mitigation
- Microbial Natural Products and Biosynthesis
- Cancer Genomics and Diagnostics
- Environmental remediation with nanomaterials
- Hydrogen's biological and therapeutic effects
- Microbial Metabolism and Applications
- Supercapacitor Materials and Fabrication
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Medical and Biological Ozone Research
- Iron oxide chemistry and applications
- Microbial Community Ecology and Physiology
- Therapeutic Uses of Natural Elements
- Genetics, Bioinformatics, and Biomedical Research
Karlsruhe Institute of Technology
2011-2024
University of Freiburg
2010
Abstract Background Some microorganisms can respire with extracellular electron acceptors using an extended transport chain to the cell surface. This process be applied in bioelectrochemical systems which organisms produce electrical current by respiring anode as acceptor. These apply flavin molecules cofactors facilitate one-electron transfer catalyzed terminal reductases and some cases endogenous shuttles. Results In model organism Shewanella oneidensis , riboflavin production excretion...
ABSTRACT Bacterial diheme c -type cytochrome peroxidases (BCCPs) catalyze the periplasmic reduction of hydrogen peroxide to water. The gammaproteobacterium Shewanella oneidensis produces peroxidase CcpA under a number anaerobic conditions, including dissimilatory iron-reducing conditions. We wanted understand function this protein in organism and its putative connection electron transport chain ferric iron. was isolated tested for activity, structural conformation analyzed by X-ray...
A Gram-positive, irregular rod-shaped, non-motile, yellow-pigmented bacterium, strain JG 31(T), was isolated in the course of identifying chromium-resistant soil bacteria. 16S rRNA gene sequence analysis bacterium indicated its phylogenetic position within genus Leucobacter. Binary alignments with 11 species recognized at time writing revealed similarities more than 97 % Leucobacter alluvii (GenBank accession no: AM072820; 99.4 %), iarius (AM040493; 98.2 aridicollis (AJ781047; 97.8 komagatae...
Chromate is a highly toxic oxyanion. Extensive industrial use and inadequate waste management has caused the pollution of several field sites. Understanding chromate resistance mechanisms that enable organisms to thrive under these conditions fundamental develop (micro)biological strategies applications aiming at bioremediation contaminated soils or waters. Potential detoxifying microorganisms are often not sufficient in their characteristics effectively perform, e.g., reduction biosorption....
Microbial dissimilatory iron reduction is an important biogeochemical process. It physiologically challenging because occurs in soils and sediments the form of insoluble minerals such as hematite or ferrihydrite. Shewanella oneidensis MR-1 evolved extended respiratory chain to cell surface reduce minerals. Interestingly, organism a similar strategy for dimethyl sulfoxide (DMSO), which reduced at well. has already been established that electron transfer through outer membrane accomplished via...
A future bioeconomy relies on the development of technologies to convert waste into valuable compounds. We present here an attempt design a biotechnological cascade for conversion vegetable acetoin and electrical energy.A dark fermentation effluent containing mainly acetate, butyrate propionate was oxidized in bioelectrochemical system. The achieved average current at constant anode potential 0 mV against standard hydrogen electrode 177.5 ± 52.5 µA/cm2. During this step, acetate were removed...
Here we present the draft genome of Leucobacter chromiiresistens. This is first sequence an organism belonging to genus Leucobacter. L. chromiiresistens was sequenced due its capability tolerate up 300 mM Cr(VI) in medium, which so far a unique feature for microorganisms.
Ozonated oil has a long tradition in medical therapy. Here, the results from previous studies regarding antibacterial effects of ozonated oils were compared. In addition, aim this study was to further examine activity against Escherichia coli for two different oils, regard ozone exposure time and amount. Using agar dilution, minimum inhibitory concentration studied. For all tested concentrations with dilution method, bacterial growth observed. Furthermore, combined spread-plating determine...
Abstract Single cell genomics (SCG) is a complementary method to metagenomics for exploring the genomes of uncultivated microorganisms. However, due minute amounts DNA in individual microbial cell, an amplification step required before sequencing. Unfortunately, this reaction notoriously costly and does not amplify all genomic regions equally well, preventing high-throughput applications leading incomplete biased genomes. Here, we show simple volume reduction approach make SCG more feasible.
Shewanella oneidensis MR-1 ist einer der Modellorganismen zur Erforschung dissimilatorischen Eisenreduktion (DIR). Zur Etablierung von extrazellularem Elektronentransfer die Ubertragung Elektronen durch das Periplasma Gram-negativer Bakterien obligatorisch. Diese Arbeit untersucht Vorgange, periplasmatischen Elektronentransport in S. vermitteln.
Abstract Some microorganisms can respire with extracellular electron acceptors using an extended transport chain to the cell surface. These organisms apply flavin molecules as cofactors facilitate one-electron transfer catalysed by terminal reductases and endogenous shuttles. In model organism Shewanella oneidensis , riboflavin production excretion triggers a specific biofilm formation response that is initiated at threshold concentration, similar canonical quorum sensing molecules....