In the study, papain was chosen from five proteases to hydrolyze proteins of monkfish swim bladders for effectively utilizing (Lophius litulon) processing byproducts, and hydrolysis conditions were optimized as temperature 65 °C, pH 7.5, enzyme dose 2.5% time 5 h using single-factor orthogonal experiments. Eighteen peptides purified bladder hydrolysate by ultrafiltration gel permeation chromatography methods identified YDYD, QDYD, AGPAS, GPGPHGPSGP, GPK, HRE, GRW, ARW, GPTE, DDGGK, IGPAS,...

In this study, we investigate the ameliorating functions of QDYD (MSP2), ARW (MSP8), DDGGK (MSP10), YPAGP (MSP13) and DPAGP (MSP18) from monkfish swim bladders on an FFA-induced NAFLD model HepG2 cells. The lipid-lowering mechanisms revealed that these five oligopeptides can up-regulate expression phospho-AMP-activated protein kinase (p-AMPK) proteins to inhibit sterol regulatory element binding protein-1c (SREBP-1c) increasing lipid synthesis up-regulating PPAP-α CPT-1 promoting β-oxidation...

ABSTRACT The complete DNA sequence of grouper iridovirus (GIV) was determined using a whole-genome shotgun approach on virion DNA. circular form genome 139,793 bp in length with 49% G+C content. It contained 120 predicted open reading frames (ORFs) coding capacities ranging from 62 to 1,268 amino acids. A total 21% (25 120) GIV ORFs are conserved the other five sequenced genomes, including replication, transcription, nucleotide metabolism, protein modification, viral structure, and...

Cataplexy, a symptom associated with narcolepsy, represents unique dissociation of behavioural states. During cataplectic attacks, awareness the environment is maintained, as in waking, but muscle tone lost, REM sleep. We have previously reported that, narcoleptic dog, noradrenergic cells locus coeruleus cease discharge during cataplexy. In current study, we report on activity serotonergic dorsal raphe nucleus. The patterns across sleep-waking states did not differ from those and recorded...

Developing CO2-tolerant cathode materials is crucial for ensuring the optimum performance and long-term durability of solid oxide fuel cells (SOFC), particularly under realistic operating conditions. In this work, a surface engineering approach employed to selectively produce defects on La0.6Sr0.4Co0.2Fe0.8O3−δ (LSCF) with integrated perovskite bulk structure. The mitigates Sr segregation optimizes local coordination environment active Co/Fe sites. modified LSCF exhibits enhanced...

Reports show that poor indoor air quality can be harmful to vulnerable groups and lead various health problems. To address this problem, work proposes a management architecture for enhancing by integrating the analytical learning models regulation of outdoor pollutant concentrations, which coordinates activation or deactivation control devices. The proposed system incorporates predictive calibration functionalities enhance overall stability effectiveness. This tests prediction accuracy...

Abstract Background: The group assignment of chronic obstructive pulmonary disease (COPD) may differ depending on whether the COPD assessment test (CAT) or modified Medical Research Council dyspnoea scale (mMRC) is used. Aims: This study intended to clarify how different patient characteristics influence differences, determine relationships between CAT and mMRC characterise patients by both mMRC. Methods: was a retrospective, cross-sectional study. data, collected Taiwan Obstructive Lung...

Treatment success rates for Mycobacterium avium complex (MAC) diseases range from 50% to 55% only. To explore effective antimicrobials against either intracellulare or M. avium, we determined in vitro activities of five aminoglycosides, clofazimine, dapsone and d-cycloserine compared with primary (clarithromycin) secondary (moxifloxacin linezolid) antimycobacterial agents. 83 non-duplicate clinical MAC isolates were collected sputum identified at the species level by PCR restriction enzyme...

Introduction: We hypothesized that hypertensive patients harbor a higher risk of urinary bladder (UB) cancer. Material and methods: performed population-based cohort study on adults using National Health Insurance Research Database (NHIRD) dataset. Hypertension comparison non-hypertensive (COMP) groups comprising 39,618 each were propensity score-matched by age, sex, index date, medical comorbidities. The outcome was incident UB cancer validated procedure codes. constructed multivariable Cox...

We propose a realistic hybrid classical-quantum linear solver to solve systems of equations specific type, and demonstrate its feasibility with Qiskit on IBM Q systems. This algorithm makes use quantum random walk that runs in [Formula: see text](N log(N)) time circuit made text](log(N)) qubits. The input output are classical data, so can be easily accessed. It is robust against noise, ready for implementation applications such as machine learning.

(1) Background: We aimed to evaluate hearing benefits from hyperbaric oxygen (HBO) therapy in patients with Idiopathic Sudden Sensorineural Hearing Loss (ISSHL). (2) Methods: performed a retrospective analysis of chart reviews on ISSHL between Jan 2016 and Dec 2021. All were referred receive HBO by the department Ear, Nose Throat (ENT). gain was assessed based pure-tone audiometry (PTA). Data analyzed for 102 after 1 5 sessions, 46 6 10 sessions. (3) Results: After 1−5 (N = 102) showed an...

Following corneal epithelium scratches, mouse corneas were infected with the multidrug resistant (MDR) P. aeruginosa strain PA54. 24 hours later, 0% (for control group), 0.01%, 0.05% or 0.1% Chlorin e6 (Ce6), a second generation photosensitizer derived from chlorophyll, was combined red light, for photodynamic inactivation (PDI). 1 hour 2 days entire eyes enucleated and homogenized counting colony forming units (CFU) of aeruginosa. For comparison, Ce6 mediated PDI started at 12 post...

The interaction among obstructive sleep apnea syndrome (OSAS) severity, sex, and obesity on cardiovascular risk as determined by serum levels of C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) remains unclear. Therefore, this study aimed to analyze individual associations between these three OSAS characteristics biomarkers determine whether the relationship was affected other features in patients with suspected OSAS. For all participants (n = 100), severity...

Metrology delay is a natural problem in the implementation of advanced process control scheme semiconductor manufacturing systems. It very important to understand effect metrology on performance In this paper, influences both transient and asymptotic properties product quality are analyzed for case when linear system with an initial bias stochastic autoregressive moving average (ARMA) disturbance under exponentially weighted (EWMA) run-to-run control. Tuning guidelines developed based study...

This paper proposes a neural fuzzy evaluation system (NFES) with significant variables selected from stepwise regression to predict apnea-hypopnea index (AHI) for evaluating obstructive sleep apnea (OSA). The considered are the change statuses of blood pressure (BP) before going and early in morning as well other five easily available measurements (age, body mass (BMI), etc.) so that users can use self-evaluation OSA. A total 150 subjects reviewed retrospectively categorized training (120...

As one core component in hydrogen fuel cells and water electrolysis cells, bipolar plates (BPs) perform multiple important functions, such as separating the oxidant flow, providing mechanical support, conducting electricity heat, connecting cell units into a stack, etc. On path toward commercialization, manufacturing costs of have to be substantially reduced by adopting low-cost easy-to-process metallic materials (e.g., stainless steel, aluminum or copper). However, these are susceptible...

To analyze the concentration-dependent effects of autologous serum (AS) and fetal bovine (FBS) on human corneal epithelial cell (HCEC) viability, migration proliferation.AS was prepared from 13 patients with non-healing defects Dulbecco's modified eagle medium/Ham's F12 (DMEM/F12) 5% FBS, 0.5% dimethyl sulphoxide (DMSO), 10 ng/mL epidermal growth factor, 1% insulin-transferrin-selenium, then were incubated in media: DMEM/F12 supplemented by 5%, 10%, 15% or 30% AS FBS. HCEC viability analyzed...

Human corneal endothelial cells (HCECs) form a monolayer covering the inner side of cornea. Since HCECs have lower mitotic activity after birth, damage or disease will lead to failure cellular pump functions and inevitably stroma oedema loss vision. Ex vivo cultured HCEC transplantation is most promising therapy used avoid donor tissue shortages. However, proliferation functional adult difficult achieve using standard cell culture techniques. In this study, we isolated sphere colonies from...

Several formulas predicting optimal continuous positive airway pressure (CPAP) for obstructive sleep apnea treatment have been developed and diverse parameters selected as predictive factors in different laboratories using ethnic groups. This study aimed to validate a constructed formula the laboratory test hypothesis that should their own formulas.Fifty-seven adult subjects with syndrome (OSAS) were enrolled model-building set underwent two polysomnography (PSG) studies diagnose OSAS...