A5078784809- Gut microbiota and health
- Bacterial Genetics and Biotechnology
- Heat shock proteins research
- Clostridium difficile and Clostridium perfringens research
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Protein Structure and Dynamics
- Probiotics and Fermented Foods
- Antibiotic Resistance in Bacteria
- thermodynamics and calorimetric analyses
- Endoplasmic Reticulum Stress and Disease
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Pharmacological Effects of Natural Compounds
- Escherichia coli research studies
- Liver Disease and Transplantation
- Gene Regulatory Network Analysis
- CRISPR and Genetic Engineering
- Liver Disease Diagnosis and Treatment
- Biochemical and Molecular Research
- Enzyme Structure and Function
- Drug Transport and Resistance Mechanisms
- Bacteriophages and microbial interactions
- Single-cell and spatial transcriptomics
- Aquaculture disease management and microbiota
Yale University
2017-2025
Lawrence Livermore National Laboratory
2024
Microbial ID (United States)
2021
University of California, San Francisco
2008-2014
University of California, Santa Cruz
2006
Summary Cyclic di‐guanylic acid (c‐diGMP) is a second messenger that modulates the cell surface properties of several microorganisms. Concentrations c‐diGMP in are controlled by opposing activities diguanylate cyclases and phosphodiesterases, which carried out proteins harbouring GGDEF EAL domains respectively. In this study, we report cellular levels higher Vibrio cholerae rugose variant compared with smooth variant. Modulation overexpressing or increased decreased colony rugosity Several...
The bacterial heat shock transcription factor, σ32, maintains proper protein homeostasis only after it is targeted to the inner membrane by signal recognition particle (SRP), thereby enabling integration of folding information from both cytoplasm and cell membrane.
ABSTRACT Vibrio cholerae switches between free-living motile and surface-attached sessile lifestyles. Cyclic diguanylate (c-di-GMP) is a signaling molecule controlling such lifestyle changes. C-di-GMP synthesized by cyclases (DGCs) that contain GGDEF domain degraded phosphodiesterases (PDEs) an EAL or HD-GYP domain. We constructed in-frame deletions of all V. genes encoding proteins with and/or domains screened mutants for altered motility phenotypes. Of 52 tested, four exhibited increase in...
Human gut Bacteroides species exhibit strain-level differences in their physiology, ecology, and impact on human health disease. However, existing approaches for genetic manipulation generally require construction of genetically modified parental strains each microbe interest or defined medium formulations. In this report, we introduce a robust efficient strategy targeted diverse wild-type from the gut. This system enables investigation members animal microbiomes beyond model organisms.
Antagonistic interactions play a key role in determining microbial community dynamics. Here, we report that one of the most widespread contact-dependent effectors human gut microbiomes, Bte1, directly targets PpiD-YfgM periplasmic chaperone complex related microbes. Structural, biochemical, and genetic characterization this interaction reveals Bte1 reverses activity complex, promoting substrate aggregation toxicity. Using Bacteroides, show is active mammalian gut, conferring fitness...
In Vibrio cholerae, the second messenger 3',5'-cyclic diguanylic acid (c-di-GMP) regulates several cellular processes, such as formation of corrugated colony morphology, biofilm formation, motility, and virulence factor production. Both synthesis degradation c-di-GMP in cell are modulated by proteins containing GGDEF and/or EAL domains, which function a diguanylate cyclase phosphodiesterase, respectively. The expression two genes, cdgC mbaA, encode harboring both domains is higher rugose...
The microbiome affects development and activity of the immune system, may modulate therapies, but there is little direct information about this control in vivo. We studied how regulation human cells humanized mice. When mice were treated with a cocktail 4 antibiotics, was an increase frequency effector T gut wall, circulating levels IFN-γ, appearance anti-nuclear antibodies. Teplizumab, non–FcR-binding anti-CD3ε antibody, no longer delayed xenograft rejection. An CD8+ central memory IL-10,...
Efficient interbacterial competitions and diverse defensive strategies employed by various bacteria play a crucial role in acquiring hold within dense microbial community. The gut symbiont Bacteroides fragilis secretes an antimicrobial ubiquitin homologue (BfUbb) that targets essential periplasmic PPIase to drive intraspecies bacterial competition. However, the mechanisms which BfUbb enters periplasm its potential for interspecies antagonism remain poorly understood. Here, we employ...
The intestinal microbiome and bacterial translocation (BT), the passage of microorganisms from gut lumen to mesenteric lymph nodes other extra-intestinal sites, are main mechanisms implicated in liver injury further decompensation patients with cirrhosis. We hypothesized that obeticholic acid (OCA), a semisynthetic bile acid, would change composition reduce experimental Rats cirrhosis induced by carbon tetrachloride inhalation (a nonseptic model) ascites present for at least 7 days were...
Abstract Overlapping genes–wherein two different proteins are translated from alternative frames of the same DNA sequence–provide a means to stabilize an engineered gene by directly linking its evolutionary fate with that overlapped gene. However, creating overlapping pairs is challenging as it requires redesign both protein products accommodate overlap constraints. Here, we present new “ o verlapping, lternate- f rame insertion” (OAFI) method for genes insertion “inner” gene, encoded in...