A5058043055- Microbial Natural Products and Biosynthesis
- Glycosylation and Glycoproteins Research
- Advanced SAR Imaging Techniques
- Ubiquitin and proteasome pathways
- Synthetic Aperture Radar (SAR) Applications and Techniques
- Enzyme Structure and Function
- Carbohydrate Chemistry and Synthesis
- Biochemical and Molecular Research
- Galectins and Cancer Biology
- Advanced Proteomics Techniques and Applications
- Chemical Synthesis and Analysis
- Advanced Image Fusion Techniques
- Click Chemistry and Applications
- Remote-Sensing Image Classification
- Endoplasmic Reticulum Stress and Disease
- Antimicrobial Peptides and Activities
- Bladder and Urothelial Cancer Treatments
- Infrared Target Detection Methodologies
- Bacterial Genetics and Biotechnology
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
- Underwater Acoustics Research
- Monoclonal and Polyclonal Antibodies Research
- Alkaloids: synthesis and pharmacology
- Microbial Applications in Construction Materials
Wuhan University
2023-2025
Nanjing University of Aeronautics and Astronautics
2024
Jinzhou Medical University
2024
Soochow University
2024
First Affiliated Hospital of Soochow University
2024
Shandong University
2021-2024
Georgia Institute of Technology
2020-2023
University of Aberdeen
2014-2022
State Key Laboratory of Microbial Technology
2021
AID Atlanta
2020-2021
Abstract Pyrrolizidine alkaloids (PAs) are a group of natural products with important biological activities. The discovery and characterization the multifunctional FAD‐dependent enzyme LgnC is now described. shown to convert indolizidine intermediates into pyrrolizidines through an unusual ring expansion/contraction mechanism, catalyze biosynthesis new bacterial PAs, so‐called legonmycins. By genome‐driven analysis, heterologous expression, gene inactivation, legonmycins were also originate...
The varying polarimetric orientation angles (POAs) result in scattering diversity, leading to ambiguity the interpretation of synthetic aperture radar (PolSAR) images. Exploring characteristics rotation domain (PRD) and complementary features can help overcome ambiguity. To address this, we propose a novel PolSAR image classification algorithm called learning similarity texture-based attention with convolutional neural networks (LSTCNNs). Three strategies are included proposed method. First,...
The subcellular localization of proteins is critical for their functions in eukaryotic cells and tightly correlated with protein modifications. Here, we comprehensively investigate the nuclear-cytoplasmic distributions phosphorylated, O-GlcNAcylated, non-modified forms to dissect correlation between distribution Phosphorylated O-GlcNAcylated have overall higher nuclear than ones. Different among are associated size, structure, function, as well local environment adjacent residues around...
(2<italic>R</italic>3<italic>S</italic>4<italic>S</italic>)-5-Fluoro-2,3,4-trihydroxypentanoic acid (5-FHPA) has been discovered as a new fluorometabolite in the soil bacterium <italic>Streptomyces</italic> sp. MA37.
<italic>Streptomyces xinghaiensis</italic> is the first fluorometabolite producing microorganism identified from marine environment.
Abstract Non-Ribosomal Peptide Synthetases (NRPSs) assemble a diverse range of natural products with important applications in both medicine and agriculture. They consist several multienzyme subunits that must interact each other highly controlled manner to facilitate efficient chain transfer, thus ensuring biosynthetic fidelity. Several mechanisms for transfer are known NRPSs, promoting structural diversity. Herein, we report the first biochemically characterized example type II...
Oriented object detection has become a hot topic in SAR image interpretation. Due to the unique imaging mechanism, objects are represented as clusters of scattering points surrounded by coherent speckle noise, leading blurred outlines and increased false alarms complex scenes. To address these challenges, we propose novel noise-to-convex paradigm with hierarchical framework based on scattering-keypoint-guided diffusion transformer (SKG-DDT), which consists three levels. At bottom level,...
Protein O-GlcNAcylation plays critical roles in many cellular events, and its dysregulation is related to multiple diseases. Integrating bioorthogonal chemistry multiplexed proteomics, we systematically site specifically study the distributions dynamics of protein nucleus cytoplasm human cells. The results demonstrate that O-GlcNAcylated proteins with different functions have distinct distribution patterns. vary specifically, indicating glycoforms same may distributions. Moreover,...
A new strategy for the identification of known compounds in Streptomyces extracts that can be applied discovery natural products is presented. The incorporates screening a database 5555 including 5098 structures from sp., using high-throughput LCMS data processing algorithm utilizes HRMS and predicted LC retention times (tR) as filters rapid product extract. database, named StrepDB, contains each compound structure, molecular formula, mass, time. All identified are annotated color coded...
Abstract β-Hydroxy-α-amino acids (βH-AAs) are key components of many bioactive molecules as well exist specialised metabolites. Among these βH-AAs, 4-fluorothreonine (4-FT) is the only naturally occurring fluorinated AA discovered thus far. Here we report overexpression and biochemical characterisation transaldolase from Streptomyces sp. MA37 (FTaseMA), a homologue FTase previously identified in biosynthesis 4-FT S. cattleya . FTaseMA displays considerable substrate plasticity to generate...
Proteins are continuously synthesized during cell growth and proliferation. At the same time, excessive misfolded proteins have to be degraded, otherwise they a burden cells. Protein degradation is essential maintain proteostasis in cells, dysfunction of protein systems results numerous diseases such as cancer neurodegenerative diseases. Despite importance degradation, pathways many remain explored. Here, we comprehensively investigated newly human cells by integrating metabolic labeling,...
Protein synthesis is quickly and tightly regulated in cells to adapt the ever-changing extracellular intracellular environment. Accurate quantitation of rapid protein changes can provide insights into functions cellular activities, but it very challenging achieve because lack effective analysis methods. Here, we developed an mass spectrometry-based method named quantitative O-propargyl-puromycin tagging (QOT) by integrating (OPP) labeling, bioorthogonal chemistry, multiplexed proteomics for...
Glycoproteins secreted by cells play essential roles in the regulation of extracellular activities. Secreted glycoproteins are often reflective cellular status, and thus from easily accessible bodily fluids can serve as excellent biomarkers for disease detection. Cultured have been extensively employed models research fields biology biomedicine, global analysis these provides insights into activities glycoprotein functions. However, comprehensive identification quantification is a daunting...
Efficient interbacterial competitions and diverse defensive strategies employed by various bacteria play a crucial role in acquiring hold within dense microbial community. The gut symbiont Bacteroides fragilis secretes an antimicrobial ubiquitin homologue (BfUbb) that targets essential periplasmic PPIase to drive intraspecies bacterial competition. However, the mechanisms which BfUbb enters periplasm its potential for interspecies antagonism remain poorly understood. Here, we employ...
In this study, a new dithiolopyrrolone biosynthetic pathway was identified in Saccharothrix algeriensis NRRL B-24137, which reported to produce variety of natural products including thiolutin, potential drug candidate for tumor angiogenesis inhibition. Bioinformatics analysis the cluster revealed that it contains all essential genes holothin core biosynthesis and several other auxiliary genes. Interestingly, heterologous expression gene Streptomyces albus only induced production holomycin,...
Abstract Background The Fujian oyster Crassostrea angulata is an economically important species that has typical settlement and metamorphosis stages. development of the involves complex morphological physiological changes, molecular mechanisms which are as yet unclear. Results In this study, changes in proteins were investigated during larval using epinephrine induction. Protein abundance identity characterized label-free quantitative proteomics, tandem mass spectrometry (MS/ MS), Mascot...
Glycoproteins on the surface of immune cells play extremely important roles in response to pathogens. Yet, a systematic and time-resolved investigation glycoproteins during remains be explored. Integrating selective enrichment with multiplexed proteomics, we globally site-specifically quantified dynamics THP-1 monocytes macrophages bacterial infection monocyte-to-macrophage differentiation. The analysis reveals transient changes differential remodeling both cell types, potential upstream...
More than 500 siderophores are known to date, but only three were identified be aryl-containing hydroxamate siderophores, legonoxamines A and B from <italic>Streptomyces</italic> sp. MA37, aryl ferrioxamine 2 <italic>Micrococcus luteus</italic> KLE1011.
Despite significant research regarding metastasis, there has been limited success in preventing it. However, gold nanoparticle (AuNP) technology shown the potential to inhibit metastasis. Our earlier studies of nanorod-assisted plasmonic photothermal therapy (AuNRs-PPTT), where nanorods (AuNRs) were irradiated with near-infrared (NIR) light induce heat, utilized slowing cancer cell migration vitro. Herein, we have expanded vitro AuNRs-PPTT xenograft mice metastasis mammary gland tumors. The...
Abstract Pyrrolizidine alkaloids (PAs) are a group of natural products with important biological activities. The discovery and characterization the multifunctional FAD‐dependent enzyme LgnC is now described. shown to convert indolizidine intermediates into pyrrolizidines through an unusual ring expansion/contraction mechanism, catalyze biosynthesis new bacterial PAs, so‐called legonmycins. By genome‐driven analysis, heterologous expression, gene inactivation, legonmycins were also originate...
Abstract Manipulation of protein synthesis is commonly applied to uncover functions and cellular activities. Multiple inhibitors with distinct mechanisms have been widely investigated employed in bio-related research, but it extraordinarily challenging measure evaluate the inhibition efficiencies individual proteins by different at proteome level. Newly synthesized are immediate direct products synthesis, thus their comprehensive quantification provides a unique opportunity study inhibition....