A5054892055- Chronic Lymphocytic Leukemia Research
- Cancer Immunotherapy and Biomarkers
- Acute Lymphoblastic Leukemia research
- Ubiquitin and proteasome pathways
- Acute Myeloid Leukemia Research
- CAR-T cell therapy research
- Glycosylation and Glycoproteins Research
- Hemoglobinopathies and Related Disorders
- Metabolomics and Mass Spectrometry Studies
- Prostate Cancer Treatment and Research
- Prostate Cancer Diagnosis and Treatment
- Biosimilars and Bioanalytical Methods
- Extracellular vesicles in disease
- Cancer Research and Treatments
- Erythrocyte Function and Pathophysiology
- Advanced Breast Cancer Therapies
- Calcium signaling and nucleotide metabolism
- Aortic Thrombus and Embolism
- Genetics and Neurodevelopmental Disorders
- Cancer-related Molecular Pathways
- Mass Spectrometry Techniques and Applications
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Hormonal and reproductive studies
- Monoclonal and Polyclonal Antibodies Research
- Pericarditis and Cardiac Tamponade
University of Perugia
2020-2025
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2022-2023
Azienda Ospedaliera Ospedale Maggiore
2023
Misericordia University
2023
Chimeric antigen receptor (CAR) T cells represent a revolutionary immunotherapy that allows specific tumor recognition by unique single-chain fragment variable (scFv) derived from monoclonal antibodies (mAbs). scFv selection is consequently fundamental step for CAR construction, to ensure accurate and effective signaling toward binding. However, conventional in vitro vivo biological approaches compare different scFv-derived CARs are expensive labor-intensive. With the aim predict finest...
Abstract Richter’s transformation (RT) is an aggressive lymphoma occurring upon progression from chronic lymphocytic leukemia (CLL). Despite advances in deciphering the RT genetic architecture, mechanisms driving this disease remain unknown. BCOR disruptive mutations were found CLL and frequently associated with NOTCH1 aberrations, a common feature RT. We engineered mice to knock-out Bcor B cells of Eμ- TCL1 mice. loss resulted alterations cell compartment favored into reduced survival...
Constitutive activation of NOTCH1-wild-type (NT1-WT) signaling is associated with poor outcomes in chronic lymphocytic leukemia (CLL), and NOTCH1 mutation (c.7541_7542delCT), which potentiates signaling, worsens the prognosis. However, specific mechanisms deregulation are still poorly understood. Accumulative evidence mentioned endoplasmic reticulum (ER) stress/unfolded protein response (UPR) as a key targetable pathway CLL. In this study, we investigated impact on CLL cell to ER stress...
Chronic lymphocytic leukemia (CLL) is an incurable disorder associated with alterations in several pathways essential for survival and proliferation. Despite the advances made CLL therapy new target agents, some cases, relapses resistance could occur, making discovery of alternatives to manage refractoriness necessary. To provide therapeutic strategies CLL, we investigated anti-leukemic activity silver nanoparticles (AgNPs), whose impact on cells has been poorly explored.We studied action...
Recurrent loss-of-function mutations of BCL6 co-repressor (BCOR) gene are found in about 4% AML patients with normal karyotype and associated DNMT3a poor prognosis. Therefore, new anti-leukemia treatments mouse models needed for this combinatorial genotype. For purpose, we first generated a Bcor-/- knockout model characterized by impaired erythroid development (macrocytosis anemia) enhanced thrombopoiesis, which both features myelodysplasia/myeloproliferative neoplasms. We then created...
NOTCH1 mutations and deregulated signal have been commonly found in chronic lymphocytic leukemia (CLL) patients. Whereas the impact of on clinical course CLL has widely studied, prognostic role activation remains to be defined. Here, we analyzed NOTCH1/NOTCH2 (ICN1/ICN2) expression JAGGED1 (JAG1) 163 patients evaluated their TTFT (Time To First Treatment) OS (Overall Survival). (ICN1+) was 120/163 (73.6%) Among them, 63 (52.5%) were mutated (ICN1+/mutated) 57 (47.5%) wild type (ICN1+/WT)....
NOTCH1 alterations have been associated with chronic lymphocytic leukemia (CLL), but the molecular mechanisms underlying activation in CLL cells are not completely understood. Here, we show that GSK3β downregulates constitutive levels of active intracellular domain (N1-ICD) cells. Indeed, silencing by small interfering RNA increases N1-ICD levels, whereas expression an mutant reduces them. Additionally, inhibitor SB216763 enhances stability at a concentration which it also cell viability. We...
Summary In chronic lymphocytic leukaemia (CLL) the efficacy of SARS‐CoV‐2 vaccination remains unclear as most studies have focused on humoral responses. Here we comprehensively examined and cellular responses to vaccine in CLL patients. Seroconversion was observed 55.2% with lower rate antibody titres treated T‐cell were detected a significant fraction CD4 + CD8 frequencies significantly increased independent serology higher levels cells patients under Bruton tyrosine kinase (BTK) or B‐cell...
Abstract BackgroundEvaluation of extracellular vescicles (EVs) has been identified as a potential predictive biomarker in patients treated with IO. In particular, Glypican-1 (GPC-1), was reported to be abnormally expressed BC tissues and GPC-1+ EVs resulted significantly higher than healthy controls. Triple negative (TNBC) is characterized by poor prognosis, due the lack effective treatments; this context, Immune Checkpoint Inhibitors (ICIs) are regarded promising agents; however, their...
Abstract Background Chronic lymphocytic leukemia (CLL) is an incurable disorder associated with alterations in several pathways essential for survival and proliferation. Despite the advances obtained CLL therapy new target agents, some cases relapses resistances could occur making necessary finding of alternatives to manage refractoriness. To provide therapeutic strategies CLL, we investigated anti-leukemic activity silver nanoparticles (AgNPs), whose impact on cells has been poorly...
174 Background: Despite the advent of chemotherapy and androgen-receptor signaling inhibitors (ARSi), patients with metastatic castration-resistant prostate cancer (mCRPC) still show poor prognosis reduced survival. The selective pressure induced by treatments favors activation alternative metabolic pathways that allow persistence cells in unfavorable conditions. This study aimed at assessing lipidomic profiles mCRPC, order to identify lipid species potentially useful predict for response...
Abstract Background: Increasing evidence suggests a link between T-cell senescence and tumour prognosis. In particular, high levels of circulating senescent T-lymphocytes have been also correlated with worse response to anti-cancer treatments. this perspective, therapeutic approach aimed at clearance restoring is regarded as an innovative strategy in cancer treatment currently under investigation pre-clinical clinical models. The purpose the present study characterize impact predictive...
Background: BCL6 co-repressor (BCOR) is a transcription factor involved in various biological processes including lymphoid development. BCOR disruptive mutations were found up to 2% of CLL and frequently associated with aberrations NOTCH1, one the most common genetic alterations poor prognosis Richter transformation (RT). Recent evidence also indicates that NOTCH signalling suppression during embryogenesis. These data indicate need for further investigation on role mutation its interplay...