A5074615195- Urinary Tract Infections Management
- Gut microbiota and health
- Escherichia coli research studies
- Cystic Fibrosis Research Advances
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Pediatric health and respiratory diseases
- interferon and immune responses
- Ubiquitin and proteasome pathways
- Glycosylation and Glycoproteins Research
- Microscopic Colitis
- Helicobacter pylori-related gastroenterology studies
- Pediatric Urology and Nephrology Studies
- Bladder and Urothelial Cancer Treatments
- RNA and protein synthesis mechanisms
- Bone health and treatments
- Vibrio bacteria research studies
- Magnesium in Health and Disease
- Pelvic floor disorders treatments
- Parathyroid Disorders and Treatments
- Osteomyelitis and Bone Disorders Research
- Nosocomial Infections in ICU
- Urinary and Genital Oncology Studies
- Urological Disorders and Treatments
Vanderbilt University Medical Center
2018-2024
Vanderbilt University
2017-2023
Cyclic GMP-AMP synthase is essential for innate immunity against infection and cellular damage, serving as a sensor of DNA from pathogens or mislocalized self-DNA. Upon binding double-stranded DNA, cyclic synthesizes dinucleotide that initiates an inflammatory response. Mouse studies recapitulate causative mutations in the autoimmune disease Aicardi-Goutières syndrome demonstrate ablating gene abolishes deleterious phenotype. Here, we report discovery class inhibitors identified by...
Biofilms are multicellular bacterial communities encased in a self-secreted extracellular matrix comprised of polysaccharides, proteinaceous fibers, and DNA. Organization these components lends spatial organization the biofilm community. Here we demonstrate that oxygen gradients uropathogenic Escherichia coli (UPEC) biofilms lead to spatially distinct expression programs for quinol oxidases—components terminal electron transport chain. Our studies reveal cytochrome bd -expressing...
Uropathogenic Escherichia coli (UPEC) is extremely diverse genotypically and phenotypically. Individual strains can variably carry virulence factors, making it challenging to define a molecular signature for this pathotype. For many bacterial pathogens, mobile genetic elements (MGEs) constitute major mechanism of factor acquisition. urinary E. coli, the total distribution MGEs their role in acquisition factors not well defined, including context symptomatic infection versus asymptomatic...
Cystic fibrosis (CF) is an autosomal recessive disease with significant gastrointestinal symptoms in addition to pulmonary complications. Recently approved treatments for CF, CF transmembrane conductance regulator (CFTR) modulators, are anticipated substantially improve the care of people and extend their lifespans. Prior work has shown that intestinal microbiome correlates health outcomes particularly children. Here, we study children before after CFTR modulator, ELX/TEZ/IVA. We identify...
Abstract The urinary bladder harbors a community of microbes termed the urobiome, which remains understudied. In this study, we present urobiome healthy infant males from samples collected by transurethral catheterization. Using combination enhanced culture and amplicon sequencing, identify several common bacterial genera that can be further investigated for their effects on health across lifespan. Many were shared between all suggesting consistent composition among cohort. We note that,...
ABSTRACT Sensory systems allow pathogens to differentiate between different niches and respond stimuli within them. A major mechanism through which bacteria sense in their surroundings is two-component (TCSs). TCSs for the detection of multiple lead a highly controlled rapid change gene expression. Here, we provide comprehensive list important pathogenesis uropathogenic Escherichia coli (UPEC). UPEC accounts >75% urinary tract infections (UTIs) worldwide. UTIs are most prevalent among...
The urinary bladder harbors a community of microbes termed the urobiome, which remains understudied. In this study, we present urobiome healthy infant males from samples collected by transurethral catheterization. Using combination extended culture and amplicon sequencing, identify several common bacterial genera that can be further investigated for their effects on health across lifespan. Many were shared between all suggesting consistent composition among cohort. We note that, cohort,...
The modification of lipopolysaccharide (LPS) in
We describe a case of hereditary hypophosphatemic rickets with hypercalciuria (HHRH) in 32-year-old female short stature, chronic pathologic genu valgum deformity, and knee pain who was referred to endocrinology clinic after previous inconclusive workups. present imaging spanning 10 years untreated disease. Biochemical studies showed hypophosphatemia undetectable fibroblast growth factor 23 (FGF23.) Renal ultrasound revealed bilateral medullary nephrocalcinosis despite no apparent...
While fosfomycin resistance is rare, the observation of non-susceptible subpopulations among clinical
Abstract Colonization by Clostridioides difficile is common in children with inflammatory bowel disease (IBD) and complicates both the management of IBD diagnosis C. infection (CDI). There a paucity data on rates, risk factors, outcomes associated asymptomatic colonization IBD. We enrolled prospectively followed 87 without acute gastrointestinal symptoms. Twelve patients (13.8%) tested positive for were considered to have colonization. Elevated white blood cell count was based univariate...
Abstract Antibiotic resistance has become a global threat. In addition to acquiring via horizontal gene transfer, bacteria can evade killing by temporarily modifying their cell envelope prevent antibiotic-bacterial interactions. A critical gap in knowledge is how balance the metabolic needs of altering with constant need generate energy. Cross-regulation between two signaling networks Escherichia coli increases positively charged antibiotics. We show that increased supported re-wiring...
The modification of lipopolysaccharide (LPS) in Escherichia coli and Salmonella spp . is primarily controlled by the two-component system PmrAB. LPS allows bacteria to avoid killing positively charged antibiotics like polymyxin B. We previously demonstrated that uropathogenic E. (UPEC), sensor histidine kinase PmrB also activates a non-cognate transcription factor, QseB, this activation somehow augments B tolerance UPEC. Here, we demonstrate - for first time absence canonical transcriptional...
You have accessJournal of UrologyCME1 Apr 2023MP70-11 DEFINING THE URINARY MICROBIOME OF HEALTHY INFANT BLADDER. Viktor Flores, Seth Reasoner, Leslie Peard, Benjamin Abelson, Carmila Manuel, Gerald Van Horn, Abby Taylor, Cyrus Adams, Mark John Thomas, Pope, Brock, Grace Morales, Jonathan Schmitz, Douglass Clayton, and Maria Hadjifrangiskou FloresViktor Flores More articles by this author , ReasonerSeth Reasoner PeardLeslie Peard AbelsonBenjamin Abelson ManuelCarmila Manuel HornGerald Horn...
Although non-barrier contraception is commonly prescribed, the risk of urinary tract infections (UTI) with contraceptive exposure unclear.Using data from Vanderbilt University Medical Centre's deidentified electronic health record (EHR), women ages 18-52 were randomly sampled and matched based on age length EHR. This case-control analysis tested for association between outcome using UTI-positive (UTI+) as cases upper respiratory infection+ (URI+) controls.24,563 UTI + (mean EHR: 64.2 months;...
Abstract Biofilms are multicellular bacterial communities encased in a self-secreted extracellular matrix comprised of polysaccharides, proteinaceous fibers, and DNA. Organization these components lends spatial organization to the biofilm community such that residents can benefit from production common goods, while being protected exogenous insults. Spatial is driven by presence chemical gradients, as oxygen. Here we quantified localized expression two Escherichia coli cytochrome oxidases...
Chronic osteomyelitis can be difficult to diagnose given its similar radiographic appearance other lesions. This case report describes a 48-year-old woman, who presented with left thigh pain and on radiography large disorganized sclerotic lesion involving nearly the entire femoral diaphysis, concerning for Paget disease or malignancy. Biopsy suggested chronic but did not identify causative organism. Treatment antibiotics led resolution of improvement biochemical markers. exemplifies role...
ABSTRACT Fosfomycin kills bacteria by blocking the binding of phosphoenolpyruvate (PEP) to bacterial enzyme MurA and halting peptidoglycan synthesis. While its use has increased with emergence antibiotic resistance, mechanisms leading fosfomycin resistance remain relatively unexplored. In uropathogenic Escherichia coli (UPEC) that accounts for >75% urinary tract infections (UTIs), enters cell primarily through UhpT, which transports glucose-6-phosphate (G6P) glycolysis intermediate into...