ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTGlycopeptide Synthesis by an .alpha.-Amino Acid N-Carboxyanhydride (NCA) Method: Ring-Opening Polymerization of a Sugar-Substituted NCAKeigo Aoi, Kaname Tsutsumiuchi, and Masahiko OkadaCite this: Macromolecules 1994, 27, 3, 875–877Publication Date (Print):January 1, 1994Publication History Published online1 May 2002Published inissue 1 January 1994https://pubs.acs.org/doi/10.1021/ma00081a040https://doi.org/10.1021/ma00081a040research-articleACS...

As acrylamide is a known neurotoxin for many animals and potential carcinogen humans, it came as surprise when the Swedish National Food Agency Stockholm University reported in 2002 that formed during frying or baking of foods. We report here genomic proteomic analyses on genes proteins Caenorhabditis elegans exposed to 500 mg/l acrylamide. Of 21,120 profiled, 409 were more than twofold upregulated 111 downregulated. Upregulated included encode detoxification enzymes such glutathione...

In order to analyze trigonelline, caffeine, chlorogenic acid, and their related compounds simultaneously, an HPLC method using InertSustain C18 column a mobile phase containing octanesulfonate as ion-pairing reagent under acidic condition was developed. The optimum conditions were determined be 0.1% phosphoric 4 mM octanesulfonate, 15% methanol at 35°C. Using the proposed method, nicotinic theophylline, caffeic acid in ten instant coffee samples analyzed. These analytes except for...

Well-defined (glyco)peptide-containing block copolymers were synthesized by ring-opening polymerization of l-phenylalanine N-carboxyanhydride (NCA) (2), γ-benzyl-l-glutamate NCA (3), and O-(tetra-O-acetyl-β-d-glucopyranosyl)-l-serine (6) initiated with ω-amine-terminated poly(2-methyl-2-oxazoline) (1a) poly(2-phenyl-2-oxazoline) (1b) macroinitiators in dichloromethane at 27 °C. s peptide segments the resulting poly(2-methyl-2-oxazoline)-block-poly(l-phenylalanine) (4),...

Background Electrophilic xenobiotics and endogenous products from oxidative stresses induce the glutathione S-transferases (GSTs), which form a large family within phase II enzymes over both animal plant kingdoms. The GSTs thus induced in turn detoxify these external as well internal stresses. Because are often linked to ageing damage health, induction of without causing adverse effects would be beneficial slowing down keeping healthy conditions. Methodology/Principal Findings We have tested...

Typical markers of protein aging are spontaneous post-translational modifications such as amino acid racemization (AAR) and isomerization (AAI) during the degradation peptides. The AAR AAI could significantly induce density localization plaque deposition in brain tissues. Alzheimer's disease (AD) is reliably related to formation aggregation amyloid-β peptide (Aβ) plaques human brain. No current analytical methods can simultaneously determine Aβ from AD patients. We now report a covalent...

Genipin was reacted with benzylamine and several amino acids to prepare gardenia blue (GB). The time-course of GB formation monitored by high-performance liquid chromatography (HPLC), time-of-flight mass spectrometry (LC-TOFMS), 1H 13C NMR measurements. In this experiment, we determined the molecular structures some intermediates using accurate masses additional techniques such as heteronuclear multiple bond correlation (HMBC). GBs (GB-AAs) were characterized both solid-state Interestingly,...

We aimed to establish a method extract cell-free DNA (cfDNA) from liquid samples by combining protamine solution with nucleic acid extraction reagents. Samples comprised 1 mL EDTA/2K plasma healthy individuals (n = 22) and 5, 10, 20, 40 K562 cell culture supernatants. After adding NaCl, the were incubated subsequently centrifuged. Genomic (gDNA) was extracted using High Pure PCR Template Preparation Kit. For comparison, cobas® cfDNA Sample The gDNA subjected real-time quantitative PCR. Our...

Abstract Magnetite nanoparticles of 9 nm size were synthesized by co-precipitation, hybridized with carbon dots in situ hydrothermal treatment, and chemically bound on an acid-treated nanohorn through dots. Separately, magnetite deposited nanohorn. Both composites well dispersed aqueous medium displayed magnetism. These examined for drug (doxorubicin gemcitabine) loading/release and, additionally, a dot-immobilized composite exerted the influence photodynamic/photothermal effects under laser...

ADVERTISEMENT RETURN TO ISSUEPREVCommunication to the...Communication the EditorFirst Synthesis of Glycopeptide Macromonomers and Graft-Type Sugar-Containing Polymers with Side ChainsKeigo Aoi, Kaname Tsutsumiuchi, Emiko Aoki, Masahiko OkadaView Author Information Department Applied Biological Science, Faculty Agricultural Sciences, Nagoya University, Chikusa-ku, 464-01, Japan Cite this: Macromolecules 1996, 29, 12, 4456–4458Publication Date (Web):June 3, 1996Publication History Received24...

A novel ABn-type dendrimer/linear polymer block copolymer, i.e., poly(trimethyleneimine) dendrimer-block-(polysarcosine)64 (1), was synthesized by ring-opening polymerization of sarcosine N-carboxyanhydride initiated with the 64-NH2-terminal dendrimer as a macroinitiator. 1 has narrow molecular weight distributions (Mw/Mn = 1.01–1.03, size exclusion chromatography) and controlled polysarcosine chain lengths (by varying monomer/dendrimer feed molar ratios). Small-angle neutron scattering...

Atomic force microscopic image on mica substrate, which was dipped for 10 min in an aqueous solution of fifth-generation poly(amido amine) dendrimer with hydroxyl end groups, displayed a flatter texture dendrimer-adsorbed surface. On the external contrast variation small-angle neutron scattering solutions, apparent radius gyration decreased increasing H2O content H2O/D2O mixed solvent, indicating water penetration into dendrimer. The calculation based theory and five-layer model fitted...

The prognosis of castration-resistant prostate cancer (CRPC) is technically scarce; therefore, a novel treatment for CRPC remains warranted. To this end, hyperthermia (HT) was investigated as an alternative therapy. In study, the analysis focused on association between and heat shock protein nuclear import factor "hikeshi (HIKESHI)", tolerance. Silencing HIKESHI expression 22Rv1 cells (human cell line) treated with siRNAs inhibited translocation 70 from cytoplasm to nucleus under enhanced...

Abstract A novel glycopeptide‐containing block copolymer, poly[ O ‐(tetra‐ ‐acetyl‐β‐ D ‐glucopyranosyl)‐ L ‐serine]‐ ‐poly(2‐methyl‐2‐oxazoline) ( 5 ), was synthesized by mutual termination of living polymerizations a sugar‐substituted α‐amino acid N ‐carboxyanhydride (NCA) 1 ) and 2‐methyl‐2‐oxazoline 3 ). deacetylated to provide the glycopeptide‐polyoxazoline ‐(β‐ 6

The adlayer formation and adsorption kinetics of third- fourth-generation (G3 G4) amphiphilic surface-block dendrimers with amino n-hexyl terminals (amino/hexyl), hydroxyl (hydroxyl/hexyl), N-acetyl-d-glucosamine (glucosamine/hexyl) on solid substrates were investigated using atomic force microscopy, contact angle, surface plasmon resonance spectroscopy, surface-enhanced infrared absorption spectroscopy. amino/hexyl hydroxyl/hexyl formed dimer-unit layers a surface, but the whole...

Evodiamine is a major alkaloid extracted from the fruit of Evodia fructus, which reduces diet-induced obesity in young animals. We investigated effects long-term dietary supplementation with evodiamine on obesity, insulin resistance and longevity normal ageing mice. Twelve-month-old C57BL/6J male mice were fed standard chow or without 1 10 mg per kg food maintained until death. Supplementation low dose prevented body weight gain improved glucose tolerance mice, increased AMPK phosphorylation...

Sugar-substituted α-amino acid N-carboxyanhydrides (glycoNCAs), i.e., O-(tetra-O-acetyl-β-D-glucopyranosyl)-L-serine NCA (2a) and O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-β-D-glucopyranosyl)-L-serine (2b), were successfully used for the introduction of a mono(glycopeptide) unit into each terminal primary amino group dendrimer. Well-defined dendrimer-based artificial glycoconjugates, O-(β-D-glucopyranosyl)-L-serine-persubstituted poly(amido amine) (PAMAM) dendrimer (3a)...

Abstract Peptide‐containing block copolymers poly( N ‐acetyliminoethylene)‐ ‐poly( L ‐phenylalanine)s (1) form water‐soluble large aggregates in water due to the hydrophobic and hydrogen‐bonding nature of ‐phenylalanine) block. The solution properties were studied by size exclusion chromatography, dynamic light scattering, 1 H NMR spectroscopy, transmission electron microscopy. results compared with those ‐benzoyliminoethylene)s (2) as analogous structures ‐phenylalanine). peptide‐containing...

Structures of poly(trimethyleneimine)/mono(amido amine) dendrimer with hydroxyl end groups and poly(amido galactose having glycopeptide were characterized in aqueous solutions compared that reported before (Langmuir 1999, 15, 4076). External contrast variation results small-angle neutron scattering suggested the segment density distribution dendrimer. The solvent penetration amount fifth generation dendrimers evaluated on basis five concentric layers model. Those differed among three...

Ion-trap LC/MS/MS was evaluated for use in the determination of acrylamide (AA) processed foods. Multiple reaction monitoring (MRM) analysis a series AA standard solutions containing deuterium-labeled (AA-d3) as an internal performed. A linear relationship between concentration and ratio peak area (AA/AA-d3) extracted ion chromatogram (m/z 55, 58 derived from m/z 72, 75, respectively) obtained over wide range 2-20,000 ng/mL. The quantification limit 2 In analyses 37 commercial foods,...