A5001349658- Wood Treatment and Properties
- Oral microbiology and periodontitis research
- Tree Root and Stability Studies
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Industrial Vision Systems and Defect Detection
- Bacterial Infections and Vaccines
- Textile materials and evaluations
- Bacterial Genetics and Biotechnology
- Remote Sensing and LiDAR Applications
- Natural Fiber Reinforced Composites
- Bacteriophages and microbial interactions
- Material Properties and Processing
- Bacterial biofilms and quorum sensing
- Optical measurement and interference techniques
- Oral Health Pathology and Treatment
- Vibrio bacteria research studies
- Antimicrobial Resistance in Staphylococcus
- Escherichia coli research studies
- HIV/AIDS oral health manifestations
- Bamboo properties and applications
- Dental Health and Care Utilization
- Forest ecology and management
- Genomics and Phylogenetic Studies
- Biochemical and Structural Characterization
Umeå University
2015-2025
The University of Western Australia
2024
Linnaeus University
2016-2022
Växjö Kommun
2019
University of Borås
2014
RISE Research Institutes of Sweden
2013
Marmara University
2010
Royal Dental Hospital of Melbourne
2009
Tokyo Medical University
2009
Universidade de São Paulo
2009
Staphylococcus aureus causes a wide spectrum of infections in humans, ranging from superficial cutaneous infections, the circum-oral region, to life-threatening bacteremia. It was recently demonstrated that Gram-positive organisms such as S. liberate membrane-derived vesicles (MVs), which analogously outer membrane (OMVs) Gram-negative bacteria can play role delivering virulence factors host cells. In present study we have shown cholesterol-dependent fusion MVs with plasma represents route...
ABSTRACT Aggregatibacter actinomycetemcomitans is implicated in aggressive forms of periodontitis. Similarly to several other Gram-negative species, this organism produces and excretes a cytolethal distending toxin (CDT), genotoxin associated with cell distention, G 2 cycle arrest, and/or apoptosis many mammalian types. In study, we have identified A. outer membrane vesicles (OMVs) as vehicle for simultaneous delivery multiple proteins, including CDT, into human cells. The OMV proteins were...
ABSTRACT Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive forms of periodontitis endocarditis. We recently demonstrated that outer membrane vesicles (OMVs) disseminated by A. could deliver multiple proteins, including biologically active cytolethal distending toxin (CDT), into the cytosol HeLa cells human gingival fibroblasts (HGF). In present work, we have used immunoelectron confocal microscopy analysis fluorescently labeled to further...
Summary The Salmonella typhimurium protein SlyA ST , originally described as a cytolysin, shows sequence similarities to several known bacterial regulatory proteins. A homologue the slyA St gene has been localised min 37 of Eschericia coli K‐12 chromosome and designated EC When introduced in trans on plasmid, conferred haemolytic phenotype wild‐type but not clyA ‐knockout strains E. K‐12. encodes novel haemolysin that is expressed by under tested laboratory conditions. Western Northern blot...
Machine strength grading of structural timber is based upon relationships between so called indicating properties (IPs) and bending strength. However, such applied on the market today are rather poor. In this paper, new IPs a method resulting in more precise predictions presented. The local fibre orientation face edge surfaces wooden boards was identified using high resolution laser scanning. combination with knowledge regarding basic wood material for each investigated board, grain angle...
Abstract Aggregatibacter actinomycetemcomitans is an opportunistic Gram-negative periodontopathogen strongly associated with periodontitis and infective endocarditis. Recent evidence suggests that periodontopathogens can influence the initiation progression of oral squamous cell carcinoma (OSCC). Herein we aimed to investigate effect A. -derived extracellular vesicles (EVs) on OSCC behavior compared EVs from known associate carcinogenesis. were isolated from: its mutant strains lacking...
The Gram-positive bacterium, Filifactor alocis is an oral pathogen, and approximately 50% of known strains encode a recently identified repeat-in-toxin (RTX) protein, FtxA. By assessing longitudinal Ghanaian study population adolescents (10-19 years age; mean age 13.2 years), we discovered possible correlation between deep periodontal pockets measured at the two-year follow-up, presence ftxA gene, high quantity F. . To further understand contribution FtxA in disease, used qPCR present to...
Escherichia coli K‐12 carries a gene for protein denoted ClyA or SheA that can mediate cytolytic phenotype. The is not expressed at detectable levels in most strains of E. , but overproduction suitable purification was accomplished by cloning the structural an hns mutant strain. Highly purified cytotoxic to macrophage cells culture and caused detachment lysis mammalian cells. Results from osmotic protection assays were consistent with suggestion formed pores diameter up 3 nm. Using...
Aggregatibacter actinomycetemcomitans is an oral and systemic pathogen associated with aggressive forms of periodontitis endocarditis. Outer membrane vesicles (OMVs) released by this species have been demonstrated to deliver effector proteins such as cytolethal distending toxin (CDT) leukotoxin (LtxA) into human host cells act triggers innate immunity upon carriage NOD1- NOD2-active pathogen-associated molecular patterns (PAMPs). To improve our understanding the pathogenicity-associated...
Effective utilization of structural timber requires grading and indicating properties (IPs) that are able to predict strength with high accuracy machines measure the underlying board at a speed corresponds production sawmills. The aim this research is assess performance new machine method/procedure which was recently approved for European market compare it other available techniques. novel method based on laser scanning utilizing tracheid effect, in combination data from dynamic excitation...
Background Outer membrane vesicles (OMVs) are known to release from almost all Gram-negative bacteria during normal growth. OMVs carry different biologically active toxins and enzymes into the surrounding environment. We suggest that may therefore be able transport bacterial proteases target host cells. present here an analysis of Vibrio cholerae OMV-associated protease PrtV. Methodology/Principal Findings In this study, we demonstrated PrtV was secreted wild type V. strain C6706 via II...
This paper aims to investigate the presence of Aggregatibacter actinomycetemcomitans and assess potential indicators risk severe form(s) periodontitis. A descriptive cross-sectional study 156 consecutive patients with periodontitis was conducted. Subgingival plaque samples were collected from participants. The identification A. performed using quantitative polymerase chain reaction. analysis, a chi-square test, binary logistic regression statistical evaluation performed. prevalence in this...
The Gram-positive organism Filifactor alocis is implicated in multiple oral diseases including periodontitis, and approximately 50% of known strains encode produce a recently identified repeat-in-toxin (RTX) protein, FtxA, partly homologous to the Aggregatibacter actinomycetemcomitans leukotoxin. By assessing longitudinal Ghanaian study population adolescents, we possible correlation between F. levels, ftxA gene carriage, progression clinical attachment loss (CAL). To extend knowledge on...
ABSTRACT Cytolysin A (ClyA) is a pore-forming cytotoxic protein encoded by the clyA gene of Escherichia coli K-12. Genetic analysis suggested that silenced nucleoid H-NS. Purified H-NS showed preferential binding to sequences in promoter region, as evidenced DNase I footprinting and gel mobility shift assays. Transcriptional derepression activation chromosomal :: luxAB operon fusion were seen under conditions deficiency SlyA overproduction, respectively. In H-NS-deficient bacteria neither...
Cytolysin A (ClyA) is a pore-forming cytotoxic protein encoded by the clyA gene that has been characterized so far only in Escherichia coli. Using DNA sequence analysis and PCR, we established conserved human-specific typhoid Salmonella enterica serovars Typhi Paratyphi entire locus absent many other S. serovars, including Typhimurium. The products, designated ClyA(STy) ClyA(SPa), show >/=90% amino acid identity to E. coli cytolysin A, ClyA(EC), they are immunogenically related. proteins...
ABSTRACT Cytolysin A (ClyA) is a newly discovered cytolytic protein of Escherichia coli K-12 that mediates hemolytic phenotype. We show here highly purified ClyA and ClyA-expressing E. were cytotoxic apoptogenic to fresh as well cultured human murine monocytes/macrophages.
The extracellular proteome (secretome) of periodontitis-associated bacteria may constitute a major link between periodontitis and systemic diseases. To obtain an overview the virulence potential Aggregatibacter actinomycetemcomitans, oral human pathogen implicated in aggressive periodontitis, we used combined LC-MS/MS bioinformatics approach to characterize secretome protein secretion pathways rough-colony serotype strain D7S. revealed 179 proteins secreted during biofilm growth. Further...
Abstract Background Aggregatibacter actinomycetemcomitans is an oral bacterium associated with aggressive forms of periodontitis. Increasing evidence points to a link between periodontitis and cardiovascular diseases, however, the underlying mechanisms are poorly understood. This study investigated pathogenic potential free-soluble surface material, released from live planktonic biofilm A. cells. Results By employing ex vivo insert model (filter pore size 20 nm) we demonstrated that strain...