To decipher the function of A1S_1331, named AbaF (Acinetobacter baumannii Fosfomycin efflux), one primary targets AbsR25, a small RNA A. baumannii.abaF was cloned in multicopy plasmid and expressed from its native promoter an efflux-deficient strain-Escherichia coli KAM32. Drug susceptibility, accumulation efflux ethidium bromide (EtBr) were determined this strain. abaF disrupted using homologous recombination effect on drug biofilm formation virulence studied. Expression followed by...

Summary Nitric oxide (NO), generated in large amounts within the macrophages, controls and restricts growth of internalized human pathogen, Mycobacterium tuberculosis H37Rv. The molecular mechanism by which tubercle bacilli survive macrophages is currently intense interest. In this work, we have demonstrated that dimeric haemoglobin, HbN, from M. exhibits distinct nitric dioxygenase (NOD) activity protects cellular respiration heterologous hosts, Escherichia coli smegmatis , toxic effect...

Silver has long been advocated as an effective antimicrobial. However, toxicity issues with silver have led to limited use of in nanoform, especially for food preservation. With the aim exploring combinatorial options that could increase antibacterial potency nanoparticles and reduce dosage silver, we evaluated extent synergy a combination essential oil representative (cinnamaldehyde) offer. A battery gram-positive gram-negative bacterial strains was utilized assays, extents synergism were...

Abstract: Infectious diseases cause a huge burden on healthcare systems worldwide. Pathogenic bacteria establish infection by developing antibiotic resistance and modulating the host's immune system, whereas opportunistic pathogens like Pseudomonas aeruginosa adapt to adverse conditions owing their ability form biofilms. In present study, silver nanoparticles were biofunctionalized with polymyxin B, an antibacterial peptide using facile method. The (polymyxin B-capped nanoparticles, PBSNPs)...

ABSTRACT Decades of antibiotic misuse have accelerated the emergence multi- and extensively drug-resistant bacteria. Bacterial pathogens employ several strategies such as resistance, tolerance, biofilm formation in response to extreme environments stress. Another crucial survival mechanism involves stochastic generation bacterial subpopulations known persisters, which can endure high concentrations antibiotics. Upon removal stress, these revert back their original phenotype links them...

Nutrient availability in infection niches and the ability of bacterial pathogens to alter their metabolic landscape utilize diverse carbon sources play a major role determining extent pathogenesis. The vertebrate lung is rich amino acids, such as arginine, which are available nutrient source establish infection. Arginine also used by host nitric oxide synthase synthesize oxide, against invading for tissue repair. In this study, we have focused on arginine catabolic pathway its importance...

Nitrogen containing heterocycles are widely used and investigated by pharmaceutical industry, as they important in discovery designing of new drug molecules. Drugs with a benzimidazole nucleus possess exclusive structural features electron-rich atmosphere, which enable them to bind number biologically targets result wide range activities. This has served the basis present study whereby scaffolds moiety were designed synthesized.The structures synthesized compounds confirmed physicochemical...

The truncated hemoglobins HbN and HbO of Mycobacterium tuberculosis H37Rv share little sequence similarity display structural differences in their EF-loop regions, suggesting distinct function(s) for these hemoglobins. M. was expressed Escherichia coli smegmatis as a 14.5-kDa homodimeric heme protein exhibiting nearly 50-fold (P(50) approximately 0.51) lower oxygen affinity than HbN. 40-50% remained associated with the cell membranes significantly enhanced its respiration comparison membrane...

ABSTRACT Dimeric hemoglobin (VHb) from the bacterium Vitreoscilla sp. strain C1 displays 30 to 53% sequence identity with heme-binding domain of flavohemoglobins (flavoHbs) and exhibits presence potential sites for interaction its FAD/NADH reductase partner. The intersubunit contact region VHb indicates a small interface between two monomers homodimer, suggesting that dimers may dissociate easily. Gel filtration chromatography exhibited 25 30% monomeric population VHb, at low protein...

Background The rising drug resistance in pathogenic bacteria and inefficiency of current antibiotics to meet clinical requirements has augmented the need establish new innovative approaches for antibacterial discovery involving identification novel targets inhibitors. Being obligatory bacterial growth, essential gene products are considered vital as targets. protein YidC is highly conserved among pathogens membrane insertion due which it holds immense potential a promising target therapy....

ABSTRACT With the emergence of multiple-drug-resistant pathogens, antibacterial property silver in colloidal form has emerged as a potential candidate for combating infectious diseases. A combination agents along with nanosilver could prove to be more potent due broadened spectrum possibly lower doses. In present study, facile single-step green method synthesizing nanoparticles functionalized an peptide from food-grade lactic acid bacterium is reported. The synthesized enterocin-coated...

Small RNA (sRNA) molecules are non-coding RNAs that have been implicated in regulation of various cellular processes living systems, allowing them to adapt changing environmental conditions. Till date, sRNAs not reported Acinetobacter baumannii (A. baumannii), which has emerged as a significant multiple drug resistant nosocomial pathogen. In the present study, combination bioinformatic and experimental approach was used for identification novel sRNAs. A total 31 putative were predicted by...

The rise of antibiotic resistance among skin-infecting pathogens poses an urgent threat to public health and has fueled the search for new therapies. Enhancing potency currently used antibiotics is alternative treatment infections caused by drug-resistant pathogens. In this study, we aimed identify a small molecule that can potentiate antibiotics. IITR00693 (2-aminoperimidine), novel antibacterial molecule, potentiates activity polymyxin B against Staphylococcus aureus Pseudomonas...

This study investigates the prevailing microbial communities during composting of vegetable waste, cattle manure and saw dust, in a household (250 l) batch scale Rotary Drum composter Windrow Pile. Physico-chemical parameters were analyzed to organic matter transformations. Total reduced from 63.8% 36.2% rotary drum 39.6% windrow pile composting. The C/N ratio decreased 26.52 8.89 14.33 indigenous population total heterotrophic bacteria after 20 days. However, fungal load initially increased...

Inappropriate and disproportionate use of antibiotics is contributing immensely to the development antibiotic resistance in bacterial species associated with food contamination. The natural products combination can be a potent alternative hurdle strategy inactivate foodborne pathogens. Here, we explored pro-oxidant properties essential oil linalool vitamin C copper (LVC) combating pathogens Vibrio fluvialis Salmonella enterica subsp. serovar Typhi using three-dimensional (3D) checkerboard...