A5101610344- Microbial Inactivation Methods
- Microfluidic and Bio-sensing Technologies
- Lipid Membrane Structure and Behavior
- Electrohydrodynamics and Fluid Dynamics
- RNA Research and Splicing
- RNA modifications and cancer
- Magnetic and Electromagnetic Effects
- Spectroscopy Techniques in Biomedical and Chemical Research
- Luminescence and Fluorescent Materials
- Nanopore and Nanochannel Transport Studies
- RNA Interference and Gene Delivery
- Lipid metabolism and biosynthesis
- Agriculture, Land Use, Rural Development
- Gut microbiota and health
- Spectroscopy and Quantum Chemical Studies
- Urban Agriculture and Sustainability
- Neuroscience and Neuropharmacology Research
- Cancer-related molecular mechanisms research
- Probiotics and Fermented Foods
- Food Waste Reduction and Sustainability
- Machine Learning in Healthcare
- Receptor Mechanisms and Signaling
University of Buenos Aires
2010-2023
Fundación Ciencias Exactas y Naturales
2010-2023
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2022
Buenos Aires Institute of Technology
2012-2014
Universitat de Barcelona
2010
Dimethyl sulfoxide (DMSO) has been known to enhance cell membrane permeability of drugs or DNA. Molecular dynamics (MD) simulations with single-component lipid bilayers predicted the existence three regimes action DMSO: loosening, pore formation and bilayer collapse. We show here that these modes are also reproduced in presence cholesterol bilayer, we provide a description at atomic detail DMSO-mediated process cholesterol-containing membranes. successfully explore applicability DMSO promote...
We present a numerical study of pore formation in lipid bilayers containing cholesterol (Chol) and subjected to transverse electric field. Molecular dynamics simulations 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DOPC) membranes reveal the when an field 325 mV/nm is applied. The minimum needed for membrane permeabilization strongly increases with addition above 10 mol %, reaching 750 40 % Chol. Analysis DOPC/Chol suggests this caused by substantial increment cohesion. Simulations also...
Abstract INTRODUCTION This study involved evaluating a tailored genetic counseling and testing (GCT) protocol for families at risk of autosomal dominant Alzheimer's disease (ADAD) in Latin America (LatAm), focusing on essential cultural regional adaptations. METHODS We conducted non‐randomized controlled trial among ADAD Colombia Argentina. Participants were categorized based their decision to learn status (GS), with further comparisons between mutation‐positive versus mutation‐negative...
Pores can be generated in lipid membranes by the application of an external electric field or addition particular chemicals such as dimethyl sulfoxide (DMSO). Molecular dynamics (MD) has been shown to a useful tool for unveiling many aspects pore formation both situations. By means MD simulations, we address electropores cholesterol-containing bilayers under influence DMSO. We show how combination physical and chemical mechanisms leads more favorable conditions generating membrane pores and,...
Over the past few years, there has been a focus on proteins that create separate liquid phases in intracellular environment, known as membraneless organelles (MLOs). These allow for spatiotemporal associations of macromolecules dynamically exchange within cellular milieu. They provide form compartmentalization crucial organizing key functions many cells. Metabolic processes and signaling pathways both cytoplasm nucleus are among performed by MLOs, which facilitated diverse combinations...
Pore formation in lipid bilayers subjected to a transverse electric field is studied by means of Molecular Dynamics simulations 1,2-dipalmitoyl-sn-glycero-3-phosphatidylcholine (DOPC). The physical characteristics the membrane are crucial understand electroporation conditions. For example, addition cholesterol (Chol) causes substantial increment cohesion that results an increase minimum needed for permeabilization. Instead, dimethyl sulfoxide (DMSO) known produce opposite effect on...
Abstract Over the past few years, there has been a focus on proteins that create separate liquid phases in intracellular environment, known as membraneless organelles (MLO). These allow for spatiotemporal associations of macromolecules dynamically exchange within cellular milieu. They provide form compartmentalization crucial organizing key functions many cells. Metabolic processes and signaling pathways both cytoplasm nucleus are among performed by MLOs, which facilitated diverse...