A5065870030- Cervical Cancer and HPV Research
- Genital Health and Disease
- Head and Neck Cancer Studies
- Cancer-related molecular mechanisms research
- Hepatitis B Virus Studies
- Herpesvirus Infections and Treatments
- T-cell and Retrovirus Studies
- Molecular Biology Techniques and Applications
- Reproductive tract infections research
- Urological Disorders and Treatments
- Colorectal and Anal Carcinomas
- Viral-associated cancers and disorders
- Infectious Diseases and Mycology
- Curcumin's Biomedical Applications
- Esophageal Cancer Research and Treatment
- NF-κB Signaling Pathways
- Urologic and reproductive health conditions
- Nonmelanoma Skin Cancer Studies
- Salivary Gland Tumors Diagnosis and Treatment
- Histone Deacetylase Inhibitors Research
- Kruppel-like factors research
- Polyomavirus and related diseases
- Vector-Borne Animal Diseases
- Glutathione Transferases and Polymorphisms
- Urinary and Genital Oncology Studies
Instituto do Câncer do Estado de São Paulo
2016-2025
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2016-2025
Universidade de São Paulo
1995-2025
Amazon (Germany)
2010
Universidade de Brasília
2010
Hospital Alemão Oswaldo Cruz
2009
Universidade Cidade de São Paulo
2000-2005
In order to determine geographically related intratypic variation in human papillomavirus (HPV) type 16 and 18 isolates that could be associated with lesion development, data were analysed from an ongoing cohort study of the natural course infection HPVs cervical neoplasia. Testing for was carried out by PCR molecular variants these characterized sequence analysis long control region dot blot hybridization E6 L1 genes. Tests HPV done multiple first-year specimens 1690 women enrolled a cancer...
The intratypic variability of HPVs 16 and 18 has been extensively studied used as an important tool in epidemiological studies viral transmission, persistence progression to clinically relevant cervical lesions. Infections by non-European variants are associated with increased risk for the development high grade squamous intraepithelial lesions (HSIL). Our aim was correlate molecular both HPV types persistent infection lesion outcome a cohort study conducted Brazil. We characterized...
Objective: This study assesses the associations between dendritic cells, HPV 6 and 11, Recurrent Respiratory Papillomatosis (RRP) aggressiveness. Methods: The Derkay score was calculated using information obtained from medical records. Biopsies 36 patients with juvenile RRP (JRRP) 43 adult (ARRP) were analyzed under light microscopy, their clinical data collected. Immunohistochemical analysis antibodies against CD83, CD1a, Factor XIIIa, S100 performed, inflammatory cells quantified. Data...
Oral HPV DNA and circulating tumor (ct) in plasma were evaluated as potential biomarkers for HPV-associated head neck cancer (HNC). Samples from HNC patients (n = 132), including 23 oropharyngeal cancers (OPC), non-HNC controls 10) analyzed. status was determined using a multiplex bead-based test (E7-MPG) applied to formalin-fixed paraffin-embedded (FFPE) tissues 90), 141), gargle samples oral swabs 142). detected 25.8% of tissues, 12% samples, 20.6% gargles 7% with HPV16 the most prevalent...
We compared E6/E7 protein properties of three different HPV-16 variants: AA, E-P and E-350G. Primary human foreskin keratinocytes (PHFK) were transduced with E6 E7 evaluated for proliferation ability to grow in soft agar. infected presented the lowest efficiency colony formation. AA E-350G attained higher capacity vitro transformation. observed similar degradation TP53 among variants. Furthermore, we accessed expression profile early (p5) late passage (p30) cells 84 genes commonly involved...
A significant proportion of recurrent respiratory papillomatosis (RRP) is caused by human papillomavirus type 6 (HPV-6). The long control region (LCR) contains cis-elements for regulation transcription. Our aim was to characterize LCR HPV-6 variants in RRP cases, compare promoter activity these isolates and search cellular transcription factors (TFs) that could explain the differences observed. complete from 13 analyzed. Transcriptional 5 compared using luciferase assays. Differences...
Abstract More than 100 HPV types have been described, 13 of which are classified as high‐risk due to their association with the development cervical cancer. The intratype genomic diversity HPV‐16 and ‐18 has studied extensively, while little data generated for other less common types. present study explores nucleotide variability phylogeny HPV‐31, ‐33, ‐35, ‐52, ‐58, in samples from Central Brazil. For this purpose, LCR E6 L1 genes were sequenced. Several variants these detected, some...
GOAL:To investigate the HPV prevalence and characterize expression of potential molecular surrogate markers infection in esophageal squamous cell carcinoma. MATERIALS AND METHODS:The individuals with without cancer (EC) was determined by using multiplex PCR; p16 p53 protein levels were assessed immunohistochemistry (IHC).RESULTS: High-risk (hr-HPV) found same frequency (13.8%) carcinoma (ESCC) healthy individuals.The positive 67.5% tumor tissue, 20.0% adjacent non-tumoral tissue 1.8% normal...
Vulvar intraepithelial neoplasia (VIN) is associated with human papillomavirus (HPV) infection. Curcumin a natural bioactive compound antineoplastic properties. The use of nanoparticles containing curcumin could allow better performance this in therapies. So, VIN biopsies were collected and HPV DNA detection was performed by PCR, positive samples genotyped Restriction Fragment Length Polymorphism (RFLP) HPV-16 variants determined sequencing. vulva carcinoma cells (A431) transduced E-P E-350G...
We assessed the incidence and risk factors for first detection redetection with same human papillomavirus (HPV) genotype, prevalence of cytological lesions during HPV redetections.
Background. The long control region (LCR) of human papillomavirus (HPV) regulates early gene transcription by interaction with several viral and cellular factors (TFs).
The genital skin of males hosts a diversity HPV genotypes and uncharacterized genotypes. Previously we demonstrated that specific viral genotype was not identified in 14% all specimens (i.e., unclassified specimens) using the Roche Linear Array method. Our goal to identify assess prevalence individual types among collected HIM Study population, at enrollment, examine associations with socio-demographic behavioral characteristics. Genital men were considered (HPV PCR positive, no specified)...
Abstract Cervical carcinoma is the leading cause of cancer death in Quechua indians from Jujuy (northwestern Argentina). To determine prevalence HPV‐16 variants, 106 positive cervical samples were studied, including 33 low‐grade squamous intraepithelial lesions (LSIL), 28 high‐grade (HSIL), 9 invasive (ICC), and 36 women with normal colposcopy cytology. HPV genome variability was examined L1 E6 genes by PCR‐hybridization. In a subset 20 samples, LCR fragment also analyzed PCR‐sequencing....
Association between non-European variants of human papillomavirus (HPV) type 16 (HPV-16) and HPV-18 cervical lesions has been suggested. To compare the P105 promoter activity among 6 variants, their complete long control regions (LCRs), as well that HeLa cells, were cloned upstream luciferase gene transiently transfected in C33 cells. Whereas B18-2 European variant showed lowest activity, Asian-Amerindian variant, B18-3, had highest activity. All tested more active than P97 HPV-16 prototype...