Abstract Low‐temperature (LT) stress threatens cucumber production globally; however, the molecular mechanisms underlying LT tolerance in remain largely unknown. Here, using a genome‐wide association study (GWAS), we found naturally occurring single nucleotide polymorphism (SNP) STAYGREEN ( CsSGR ) coding region at gLTT5.1 locus associated with tolerance. Knockout mutants of generated by clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR‐associated nuclease 9 exhibit...

Summary Vitamin A is a crucial yet scarce vitamin essential for maintaining normal metabolism and bodily functions in humans can only be obtained from food. Carotenoids represent diverse group of functional pigments that act as precursors vitamins, hormones, aroma volatiles antioxidants. As vital vegetable the world, elevated carotenoid levels cucumber fruit produce yellow flesh, enhancing both visual appeal nutritional value. However, genetic mechanisms regulatory networks governing flesh...

Green flesh color, resulting from the accumulation of chlorophyll, is one most important commercial traits for fruits. The genetic network regulating green formation has been studied in tomato, melon and watermelon. However, little known about inheritance molecular basis cucumber. This study sought to determine main genomic regions associated with flesh. Three F2 two BC1 populations derived 9110Gt (cultivated cucumber, color) PI183967 (wild white were used analysis. Two them further employed...

Heat stress during cucumber production often leads to sunburn of leaves, growth retardation stems and roots, fruit malformation, even plant death, which have a great impact on the quality yield. However, no studies genetic inheritance quantitative trait locus mapping heat tolerance in at adult stage been reported yet. In this study, set 86 recombinant inbred lines (RILs) derived from “99281” (heat-tolerant) “931” (heat-sensitive) were used identify QTL summer 2018, 2019, 2020. Eight-week-old...

Abstract Powdery mildew (PM) is one of the most destructive diseases that threaten cucumber production globally. Efficient breeding novel PM-resistant cultivars will require a robust understanding molecular mechanisms resistance against PM. Using genome-wide association study, we detected locus significantly correlated with PM in stem, pm-s5.1. A 1449-bp insertion CsMLO8 coding region at pm-s5.1 resulted enhanced stem resistance. Knockout mutants and CsMLO11 generated by CRISPR/Cas9 both...

High temperature is one of the major abiotic stresses that affect cucumber growth and development. Heat stress often leads to metabolic malfunction, dehydration, wilting death, which has a great impact on yield fruit quality. In this study, genetic analysis quantitative trait loci (QTL) mapping for thermotolerance in seedlings was investigated using recombinant inbred line (RILs; HR) population doubled haploid (DH; HP) derived from two parental lines ‘65G’ (heat-sensitive) ‘02245′...

Fruit spine is an important agronomic trait in cucumber and the "numerous spines (ns)" varieties are popular Europe West Asia. Although classical genetic locus of ns was reported more than two decades ago, NS gene has not been cloned yet. In this study, nine loci for different densities fruit were identified by a genome-wide association study. Among loci, fsdG2.1 closely associated with ns, which harbors candidate Csa2G264590. Overexpression Csa2G264590 resulted lower density, knockout...

Cucumber is very sensitive to salt stress, and excessive content in soils seriously affects normal growth development, posing a serious threat commercial production. In this study, the recombinant inbred line (RIL) population (from cross between tolerant CG104 CG37) was used study genetic mechanism of tolerance cucumber seedlings. At same time, candidate genes within mapping region were cloned analyzed. The results showed that seedlings quantitative trait controlled by multiple genes....

abiotic stress responses.Furthermore, expression profiling of rice ATG6 genes based on microarray data, as well by semi-quantitative RT-PCR, showed differential when subjected to different stresses suggesting the involvement OsATG6 in (heat, cold and drought) hormone (abscisic acid) responses.Analysis co-expressed that most them annotated DNA repair pathways proteolysis, etc. Collectively, these results suggest stresses, provide a basis for further functional studies investigate biological...

Cucumber (Cucumis sativus L.) is an economically important vegetable crop worldwide, but it sensitive to low temperatures. seedlings exposed long-term temperature stress (LT), i.e., below 20°C during the day, and 8°C at night, exhibit leaf yellowing, accelerated senescence, reduced yield, therefore posing a threat cucumber production. Studying underlying mechanisms involved in LT tolerance seedlings, developing germplasm with improved LT-tolerance could provide fundamental solutions problem....

Cucumber is one of the most widely consumed vegetables worldwide, and fruit spine an important quality trait. Expansins play critical roles in development; however, regulation expansins cucumber development has not been reported. In this study, 33 expansin genes were identified genome V3; additionally, Citrullus lanatus, Cucumis melo, Cucurbita maxima, Lagenaria siceraria, Benincasa hispida also identified. Phylogenetic analysis proteins Cucurbitaceae Arabidopsis showed that they evolved...

Gummy stem blight (GSB), caused by Didymella bryoniae, is a serious fungal disease that leads to decline in cucumber yield and quality. The molecular mechanism of GSB resistance remains unclear. Here, we investigated the core germplasms from four geographic groups at seedling adult stages. A total 9 SNPs related stage 26 were identified, which some are co-localized previously mapped Quantitative trait loci (QTLs) for (gsb3.2/gsb3.3, gsb5.1, gsb-s6.2). Based on haplotype analysis expression...

Salt stress seriously restricts plant growth and development, affects yield quality, thus becomes an urgent problem to be solved in cucumber resistance breeding. Mining salt tolerance genes exploring the molecular mechanism of could accelerate breeding germplasm with excellent tolerance. In this study, 220 core accessions were used for Genome-Wide Association Studies (GWAS) identification genes. The salinity injury index that was collected two years showed significant differences among...

Low temperatures (LTs) negatively affect the percentage and rate of cucumber ( Cucumis sativus L.) seed germination, which has deleterious effects on yield. Here, a genome-wide association study (GWAS) was used to identify genetic loci underlying low temperature germination (LTG) in 151 accessions that represented seven diverse ecotypes. Over two years, phenotypic data for LTG i.e., relative (RGR), energy (RGE), index (RGI) radical length (RRL), were collected environments, 17 found be...

Heat stress causes overgrowth, leaf dryness and fruit malformation, which negatively impacts cucumber quality yield. Yet, in spite of the devastating consequences this abiotic stress, few genes for heat tolerance have been identified. Here, injury indices 88 accessions representing diverse ecotypes were collected two open-field environments, with naturally occurring high temperatures over years. Seventeen identified as highly heat-tolerant. Using a genome-wide association study, five loci...

BAK1 (Brassinosteroid insensitive 1-associated receptor kinase 1) plays an important role in disease resistance plants. However, the function of family cucumber and decisive genes for disease-resistance remain elusive. Here, we identified 27 CsBAK1s cucumber, classified them into five subgroups based on phylogenetic analysis gene structure. same subgroup shared similar motifs, but different structures. Cis-elements revealed that might respond to various stress growth regulation. Three...

Starch provides plants with carbon and energy during stressful periods; however, relatively few regulators of starch metabolism under stress-induced starvation have been discovered. We studied a protein kinase Ser/Thr/Tyr (STY) 46, identified by gene co-expression network analysis as potential regulator the response in Arabidopsis thaliana. showed that STY46 was induced (1) abscisic acid prolonged darkness, (2) abiotic stressors, including salinity osmotic stress, (3) conditions associated...

The GRAS (gibberellic acid insensitive, repressor of GAI, and scarecrow) proteins are a family plant-specific transcription factors that regulate plant growth, development, stress response. Currently, the role in various abiotic responses has not been systematically studied cucumber (Cucumis sativus L.), popular vegetable crop. Here, we provide comprehensive bioinformatics analysis 35 genes identified genome. In this study, genotypes, i.e., “CG104”, which is stress-tolerant, genotype “CG37”,...