A5000829870- Neuroscience and Neuropharmacology Research
- Pluripotent Stem Cells Research
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- 3D Printing in Biomedical Research
- Receptor Mechanisms and Signaling
- Single-cell and spatial transcriptomics
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neural Engineering
Massachusetts Institute of Technology
2021-2024
Vanderbilt University
2021-2024
Day length, or seasonal photoperiod, shapes mood and affective behaviors but the neural mechanisms underlying these effects are still being defined. Serotonin neurons of dorsal raphe nucleus (DRN) critical regulators photoperiod modulates their excitability ongoing activity. Here, we investigated influence on function expression small conductance calcium activated potassium (SK) channels which mediate afterhyperpolarizing potential (AHP) in serotonin neurons. Building previous work...
The nucleus accumbens (NAc) guides reward-related motivated behavior implicated in pathological behavioral states, including addiction and depression. These behaviors depend on the precise neuromodulatory actions of G i/o -coupled G-protein-coupled receptors (GPCRs) at glutamatergic synapses onto medium spiny projection neurons (MSNs). Previous work has shown that discrete classes GPCR mobilize Gβγ to inhibit vesicular neurotransmitter release via t-SNARE protein, SNAP25. However, it remains...
N-methyl-D-aspartate (NMDA) receptors are critical for higher-order nervous system function, but in previously published protocols to convert human induced pluripotent stem cells (iPSCs) mature neurons, functional NMDA (NMDARs) often either not reported or take an extended time develop. Here, we describe a protocol iPSC-derived neural progenitor (NPCs) neurons only 37 days. We demonstrate that the express NMDARs exhibiting ligand-activated calcium flux, and document presence of...
Abstract Circular RNAs (circRNAs) are a class of closed-loop, single stranded whose expression is particularly enriched in the brain. Despite this enrichment and evidence that circRNAs altered by synaptic development response to plasticity vitro , regulation function majority experience-dependent vivo remain unexplored. Here, we employed transcriptome-wide analysis comparing differential both mRNAs juvenile mouse primary visual cortex (V1) following monocular deprivation (MD), model...