A5088235023- Reproductive Biology and Fertility
- Assisted Reproductive Technology and Twin Pregnancy
- Ovarian function and disorders
- Sperm and Testicular Function
- Prenatal Screening and Diagnostics
- Reproductive Health and Technologies
- Renal and related cancers
- Extracellular vesicles in disease
- CRISPR and Genetic Engineering
- Cardiovascular Disease and Adiposity
- Microbial Inactivation Methods
- Animal Genetics and Reproduction
- Pregnancy and preeclampsia studies
- Genomics and Chromatin Dynamics
- Endometriosis Research and Treatment
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
Nanjing Maternity and Child Health Care Hospital
2013-2025
Nanjing Medical University
2013-2025
Abstract Purpose To elucidate the characterization of extracellular vesicles (EVs) in follicular fluid-derived (FF-EVs) and discover critical molecules signaling pathways associating with etiology pathobiology PCOS, differentially expressed miRNAs (DEmiRNAs) proteins profiles (DEPs) were initially explored combinedly analyzed. Methods First, miRNA protein expression FF-EVs PCOS patients control compared by RNA-sequencing tandem mass tagging (TMT) proteomic methods. Subsequently, Gene...
Zygotic genomic activation (ZGA) is a landmark event in the maternal-to-zygotic transition (MZT), and regulation of ZGA by maternal factors remains to be elucidated. In this study, depletion ring finger protein 114 (RNF114), ubiquitin E3 ligase, led developmental arrest two-cell mouse embryos. Using immunofluorescence transcriptome analysis, RNF114 was proven play crucial role major ZGA. To study underlying mechanism, we performed profiling mature oocytes found potential substrate for...
Intrauterine insemination (IUI) is an effective, noninvasive, relatively simple and cheap method of infertility treatment. Many factors that affect IUI outcomes have been studied. However, there no consensus about the optimal number motile spermatozoa inseminated (NMSI) required for a reasonable chance pregnancy after IUI. In this retrospective study, we aimed to assess relationship between NMSI rate with husband's spermatozoa. Couples who had either primary or secondary more than one year...
To explore the need for rescue intracytoplasmic sperm injection (ICSI) in cases of partial fertilisation failure during a conventional vitro cycle, ICSI was performed cycles with rate <50%. The data were divided into three groups based on rate: group 1 (0%), 2 (<25%) and 3 (>25%). impact each then analysed terms ovum fertilisation, embryo development, utilisation selection embryos transfer. Rescue 1831 unfertilised oocytes from 313 cycles. rates 1, 74.66, 68.35 65.46%, polyploidy 8.55,...
Abstract STUDY QUESTION Does unplanned spontaneous follicular growth and ovulation affect clinical outcomes after planned artificial frozen-thawed embryo transfer (AC-FET) cycles? SUMMARY ANSWER AC-FET events resulted in notably better pregnancy with a significantly higher implantation rate (IR), (CPR), ongoing (OPR) live birth (LBR) lower miscarriage rate. WHAT IS KNOWN ALREADY The protocol without GnRH agonist administration is associated low incidence of ovulation. In the literature,...
Abstract High‐quality in vitro human embryo culture medium can improve the blastocyst formation rate and quality be beneficial for clinical application of single transfer. Mammalian embryos secrete protein products into surrounding medium. As a group bioactive molecules degraded proteins, peptides have been shown to participate various biological processes. Using liquid chromatography‐tandem mass spectrometry, we performed comparative peptidomic analysis nonblastocyst‐formation groups. A...
To investigate the incidence of functional ovarian cysts, its influence on clinical rates, and proper management after depot gonadotropin-releasing hormone (GnRH) agonist pretreatment in artificial frozen-thawed embryo transfer cycles (AC-FET).This retrospective cohort study involved 3375 AC-FET with follicular-phase GnRH administration between January 2017 December 2020. Subjects were divided into a group (cycles cyst formation) control without formation). The was matched by propensity...
In this study, we aimed to evaluate whether post-thaw culture duration affected the clinical outcomes of frozen blastocyst transfer. This retrospective cohort study included 3,901 frozen-thawed transfer cycles. The cohorts were divided into two groups based on developmental stage (day 5 [D5] and day 6 [D6]) after thawing (short culture, 2-6 h; long 18-20 h). Women in short group following D6 further three subgroups depending period (2, 4, main outcomes, namely live birth rate (LBR),...
Abstract Background: To evaluate whether post-thaw culture duration affects clinical outcomes after frozen blastocyst transfer. Methods: This retrospective cohort study included 3901 frozen-thawed transfer cycles between September 2019 and March 2021. The cohorts were divided into two groups: developmental stage [day (D) 5 D6] thawing (short of 2 to 6 h long 18 20 h). Women in the short group following D6 further assigned three subgroups depending on period (2 h, 4 main live birth rate (LBR),...