A5100396507- Neuroinflammation and Neurodegeneration Mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Immune cells in cancer
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Tissue Engineering and Regenerative Medicine
- Pancreatic function and diabetes
- Glioma Diagnosis and Treatment
- Neurological Disease Mechanisms and Treatments
- Extracellular vesicles in disease
- Cholesterol and Lipid Metabolism
- Histone Deacetylase Inhibitors Research
- Alzheimer's disease research and treatments
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Endoplasmic Reticulum Stress and Disease
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Circular RNAs in diseases
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Migraine and Headache Studies
- Protein Degradation and Inhibitors
Peking University Sixth Hospital
2016-2025
Peking University
2016-2025
University of South China
2025
Hunan University
2025
Weifang Medical University
2023-2024
Baoji City Central Hospital
2024
Sichuan University of Science and Engineering
2024
Harbin Medical University
2002-2024
First Affiliated Hospital of Harbin Medical University
2002-2024
German Center for Neurodegenerative Diseases
2024
Abstract A hallmark of nervous system aging is a decline white matter volume and function, but the underlying mechanisms leading to pathology are unknown. In present study, we found age-related alterations oligodendrocyte cell state with reduction in total density murine matter. Using single-cell RNA-sequencing, identified interferon (IFN)-responsive oligodendrocytes, which localize proximity CD8 + T cells Absence functional lymphocytes decreased number IFN-responsive oligodendrocytes...
Upon demyelinating injury, microglia orchestrate a regenerative response that promotes myelin repair, thereby restoring rapid signal propagation and protecting axons from further damage. Whereas the essential phagocytic function of for remyelination is well known, underlying metabolic pathways required debris clearance are poorly understood. Here, we show cholesterol esterification in male mouse microglia/macrophages necessary adaptive to uptake generation lipid droplets upon injury. When...
Intramuscular fat (IMF) is one of the most important factors positively associated with meat quality. Triglycerides (TGs), as main component IMF, play an essential role in muscle lipid metabolism. This transcriptome analysis pectoralis tissue aimed to identify functional genes and biological pathways likely contributing extreme differences TG content broiler chickens.The study included Jingxing-Huang broilers that were significantly different (5.81 mg/g 2.26 mg/g, p < 0.01) deposition...
Abstract Understanding the complexity of cellular function within a tissue necessitates combination multiple phenotypic readouts. Here, we developed method that links spatially-resolved gene expression single cells with their ultrastructural morphology by integrating multiplexed error-robust fluorescence in situ hybridization (MERFISH) and large area volume electron microscopy (EM) on adjacent sections. Using this method, characterized transcriptional responses glial infiltrating T-cells...
Abstract Age-related myelin damage induces inflammatory responses, yet its involvement in Alzheimer’s disease remains uncertain, despite age being a major risk factor. Using mouse model of disease, we found that amyloidosis itself triggers age-related oligodendrocyte and damage. Mechanistically, CD8 + T cells promote the progressive accumulation abnormally interferon-activated microglia display myelin-damaging activity. Thus, our data suggest immune responses against myelinating...
Combined pulmonary fibrosis and emphysema (CPFE) is increasingly acknowledged as a separate syndrome with distinct clinical, physiological radiological characteristics. We sought to identify physiologic radiographic indices that predict mortality in CPFE.Data on clinical characteristics, function, high-resolution computed tomography (HRCT) treatment were compared between patients usual interstitial pneumonia (UIP) plus (CPFE group) those IPF alone (IPF group). Composite index (CPI) HRCT...
The reduced ability of the central nervous system to regenerate with increasing age limits functional recovery following demyelinating injury. Previous work has shown that myelin debris can overwhelm metabolic capacity microglia, thereby impeding tissue regeneration in aging, but underlying mechanisms are unknown. In a model demyelination, we found substantial number genes were not effectively activated aged myeloid cells displayed epigenetic modifications associated restricted chromatin...
Mcm2-7 helicase is loaded onto double-stranded origin DNA as an inactive double hexamer (DH) in G1 phase. The mechanisms of remodeling that trigger activation S phase remain unknown. Here, we develop approach to detect and purify the endogenous DHs directly. Through cellular fractionation, provide vivo evidence are assembled on chromatin separated during Interestingly, Mcm10, a robust MCM interactor, co-purifies exclusively with context chromatin. Deletion main interaction domain, Mcm10 C...
Immunochemotherapy is viewed as a promising approach for cancer therapy via combination treatment with immune-modulating drugs and chemotherapeutic drugs. A novel dual-functional immunostimulatory polymeric prodrug carrier PEG2k-Fmoc-1-MT was developed simultaneously delivering 1-methyl tryptophan (1-MT) of an indoleamine 2,3-dioxygenase (IDO) inhibitor doxorubicin (DOX) breast immunochemotherapy. DOX/PEG2k-Fmoc-1-MT micelles were more effective in cell proliferation inhibition apoptosis...
Transient receptor potential canonical 6 (TRPC6) channel is an important non-selective cation with a variety of physiological roles in the central nervous system. Evidence has shown that TRPC6 involved process experimental stroke; however, underlying mechanisms remain unclear. In present study, role astrocytic was investigated oxygen–glucose deprivation cell model and middle cerebral artery occlusion (MCAO) mouse stroke. HYP9 (a selective agonist) SKF96365 (SKF; TRPC antagonist) were used to...
Brain tumors are the most common solid of childhood, accounting for over 20% cancers in children under 15 years age. Pilocytic astrocytomas (PAs), World Health Organization grade I, one frequently occurring childhood brain tumors, yet little is known about genetic changes characterizing this entity. We have used microarray comparative genomic hybridization at 0.97 Mb resolution to study a series PAs (n = 44). No copy number abnormality was seen 64% cases resolution. However, whole...
Effective systemic therapeutic options are limited for bladder cancer. In this preclinical study we tested whether cancer gene alterations may be predictive of treatment response.We performed genomic profiling two patient derived tumor xenografts (PDX). We optimized the exome sequence analysis method to overcome mouse genome interference.We identified a number somatic mutations, mostly shared by primary tumors and PDX. particular, BLCAb001, which is less responsive cisplatin than BLCAb002,...
In recent years, microalgae have drawn increasing attention as a valuable source of functional food ingredients. Intriguingly, Nitzschia laevis is rich in fucoxanthinol that seldom found natural sources. Fucoxanthinol, marine xanthophyll carotenoid, possesses various beneficial bioactivities. Nevertheless, it’s not clear whether could exert anti-neuroinflammatory function. light these premises, the aim present study was to investigate anti-inflammatory role purified from Lipopolysaccharide...
Abstract Background Accumulating evidence demonstrates that tRFs (tRNA-derived small RNA fragments) and tiRNAs stress-induced RNA), an emerging category of regulatory molecules derived from transfer RNAs (tRNAs), are dysregulated in various human cancer types play crucial roles. However, their roles mechanisms hepatocellular carcinoma (HCC) liver stem cells (LCSCs) still unknown. Methods The expression glycine tRNA-derived fragment (Gly-tRF) was measured by qRT-PCR. Flow cytometric analysis...