A5072188512- Microtubule and mitosis dynamics
- Retinal Development and Disorders
- Cellular transport and secretion
- CRISPR and Genetic Engineering
- 14-3-3 protein interactions
- Digestive system and related health
- Photosynthetic Processes and Mechanisms
- Neuroscience and Neuropharmacology Research
- Trace Elements in Health
- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Genomics and Chromatin Dynamics
- Alzheimer's disease research and treatments
- Synthesis and biological activity
- Nuclear Structure and Function
- Calcium signaling and nucleotide metabolism
- Chemical Reactions and Isotopes
- Endoplasmic Reticulum Stress and Disease
- Click Chemistry and Applications
- Histone Deacetylase Inhibitors Research
Institute of Cell Biology
2025
Charles University
2014-2024
The pentameric WASH complex facilitates endosomal protein sorting by activating Arp2/3, which in turn leads to the formation of F-actin patches specifically on surface. It is generally accepted that attaches membrane via interaction its subunit FAM21 with retromer VPS35. However, we observe and present endosomes even absence We show binds surface both a retromer-dependent retromer-independent manner. anchor directly mediated SWIP. Furthermore, SWIP can interact number phosphoinositide...
The osteo-oto-hepato-enteric (O2HE) syndrome is a severe autosomal recessive disease ascribed to loss-of-function mutations in the Unc-45 myosin chaperone A (UNC45A) gene. clinical spectrum includes bone fragility, hearing loss, cholestasis, and life-threatening diarrhea associated with microvillus inclusion disease-like enteropathy. Here, we present molecular functional analysis of UNC45A c.710T>C (p.Leu237Pro) missense variant, which revealed unique pathogenicity compared other genetic...
Abstract Microtubules of all eukaryotic cells are formed by α- and β-tubulin heterodimers. In addition to the well known cytoplasmic tubulins, a subpopulation tubulin can occur in nucleus. So far, potential function nuclear has remained elusive. this work, we show that β-tubulins various organisms contain multiple conserved export sequences, which targets Exportin 1/CRM1 pathway. We demonstrate exemplarily these NES motifs sufficient mediate GFP as model cargo be inhibited leptomycin B, an...
Abstract Tau, an axonal microtubule-associated protein, is a critical regulator of microtubule function and stability. Tau interaction with microtubules regulated by tau phosphorylation. hyperphosphorylation implicated in destabilization related to neurodegenerative disorders. How phosphorylation leads however unknown. Recently, it was shown that molecules on cooperatively assemble into cohesive layers termed envelopes. envelopes protect against degradation microtubule-severing enzymes,...
Abstract The pentameric WASH complex facilitates endosomal protein sorting by activating Arp2/3, which in turn leads to the formation of F-actin patches specifically on surface. It is generally accepted that attaches membrane via interaction its subunit FAM21 with retromer VPS35. However, we observe and present endosomes even absence We show binds surface both a retromer-dependent retromer-independent manner. anchor directly mediated SWIP. Furthermore, SWIP can interact number...