Spinal cord injury (SCI) results in severe disruption of communication between the brain and body, causing motor, sensory, autonomic dysfunctions. While SCI mammals leads to permanent impairment due limited regenerative capacity, certain non-mammalian species, such as Xenopus laevis larval stages, exhibit remarkable abilities. During spinal regeneration, neural stem precursor cells (NSPCs) surrounding central canal rapidly proliferate response SCI, compensating for cellular loss, restoring...

Significance Corticotropin releasing factor binding protein (CRF-BP) belongs to the CRF family that is fundamental in stress response and interaction between addiction. The mechanisms by which CRF-BP regulates system are not fully understood. Most G protein-coupled receptors (GPCRs) located mainly intracellularly depend on specific escort proteins for their trafficking cell surface. 2α R also intracellularly; however, no regulating its presence surface has been described. We show interacts...

The formation and maintenance of microtubules requires their polymerisation, but little is known about how this polymerisation regulated in cells. Focussing on the essential microtubule bundles axons Drosophila Xenopus neurons, we show that plus-end scaffold Eb1, polymerase XMAP215/Msps lattice-binder Tau co-operate interdependently to promote bundle organisation during axon development maintenance. Eb1 each other's localisation at polymerising plus-ends. outcompetes Eb1-binding along...

It has long been established that neuronal growth cone navigation depends on changes in microtubule (MT) and F-actin architecture downstream of guidance cues. However, the mechanisms by which MTs are dually coordinated remain a fundamentally unresolved question. Here, we report well-characterized MT polymerase, XMAP215 (also known as CKAP5), plays an important role mediating MT-F-actin interaction within cone. We demonstrate regulates alignment through its N-terminal TOG 1-5 domains....

The Wnt signaling pathway plays important roles during different stages of neuronal development, including polarization and dendritic axonal outgrowth. However, little is known about the identity Frizzled receptors mediating these processes. In present study, we investigated role Frizzled-5 (Fzd5) on development in cultured Sprague-Dawley rat hippocampal neurons. We found that Fzd5 expressed early neurons actin-rich structures localized at minor neurites growth cones. At 4 DIV, polarizes...

There is significant functional evidence showing that corticotropin-releasing factor type-2 receptor (CRF2R) and factor-binding protein (CRF-BP) regulate glutamatergic synapses onto ventral tegmental area (VTA) dopaminergic neurons. It has been shown CRF requires CRF-BP to potentiate N-methyl-D-aspartate receptors in neurons through CRF2R, increases glutamate release cocaine-treated rats the activation of CRF2R only by agonists with high affinity CRF-BP. Furthermore, this CRF-mediated...

Dopamine and corticotrophin-releasing hormone (CRH; also known as factor) are key neurotransmitters in the interaction between stress addiction. Repeated treatment with cocaine potentiates glutamatergic transmission rat basolateral amygdala/cortex pathway through a synergistic action of D1 -like dopamine receptors CRH type-2α (CRF2 α receptors). We hypothesized that this observed synergism could be instrumented by heteromers containing receptor CRF2 receptor.

Xenopus laevis are able to regenerate the spinal cord during larvae stages through activation of neural stem progenitor cells (NSPCs). Here we use high-resolution expression profiling characterize early transcriptome changes induced after injury, aiming identify signals that trigger NSPC proliferation. The analysis delineates a pathway starts with rapid and transitory immediate genes, followed by migration processes immune response pervasive increase NSPC-specific ribosome biogenesis...

Abstract The formation and maintenance of microtubules requires their polymerisation, but little is known about how this polymerisation regulated in cells. Focussing on the essential microtubule bundles axons Drosophila Xenopus neurons, we show that plus-end scaffold Eb1, polymerase XMAP215/Msps lattice-binder Tau co-operate interdependently to promote bundle organisation during axon development maintenance. Eb1 each other’s localisation at polymerising plus-ends. outcompetes Eb1-binding...

The corticotropin-releasing factor (CRF) system is a key mediator of the stress response and addictive behavior. CRF includes four peptides: CRF, urocortins I-III, binding protein (CRF-BP) that binds with high affinity, two class B G-protein coupled receptors CRF1R CRF2R. CRF-BP secreted without significant sequence homology to or any other known protein. Recently, it has been described potentiation role over signaling through CRF2R in addictive-related neuronal plasticity In addition,...

The mammalian guanine deaminase (GDA), called cypin, is important for proper neural development, by regulating dendritic arborization through modulation of microtubule (MT) dynamics. Additionally, cypin can promote MT assembly in vitro. However, it has never been tested whether (or other GDA orthologs) binds to MTs or modulates Here, we address these questions and characterize Xenopus laevis (Gda) the first time during embryonic development.We find that exogenously expressed human Gda both...

Spinal cord injury (SCI) is a permanent affliction, which affects the central nervous system (CNS) motor and sensory nerves, resulting in paralysis beneath site. To date, there no functional recovery therapy for SCI, lack of clarity regarding many complexes dynamic events occurring after SCI. Many non-mammalian organisms can regenerate severe such as teleost fishes, urodele amphibians, larval stages anuran including Xenopus laevis tadpoles. These are bona fide model to study understand...

Spinal cord injury (SCI) is a permanent affliction, which affects the central nervous system (CNS) motor and sensory nerves, resulting in paralysis beneath site. To date, there no functional recovery therapy for SCI, lack of clarity regarding many complexes dynamic events occurring after SCI. Many non-mammalian organisms can regenerate severe such as teleost fishes, urodele amphibians, larval stages anuran including Xenopus laevis tadpoles. These are bona fide model to study understand...