A5081596253- Neurogenesis and neuroplasticity mechanisms
- Nerve injury and regeneration
- Mitochondrial Function and Pathology
- Bioinformatics and Genomic Networks
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Neuroscience and Neuropharmacology Research
- Microbial Metabolic Engineering and Bioproduction
- Antimicrobial Peptides and Activities
- Diet and metabolism studies
- MicroRNA in disease regulation
- Chromosomal and Genetic Variations
- Signaling Pathways in Disease
- interferon and immune responses
- Genetics and Neurodevelopmental Disorders
- Molecular Biology Techniques and Applications
- Memory and Neural Mechanisms
- Amyotrophic Lateral Sclerosis Research
- Advanced biosensing and bioanalysis techniques
- Ubiquitin and proteasome pathways
- Congenital heart defects research
- Neurobiology and Insect Physiology Research
- Advanced Proteomics Techniques and Applications
- Neurogenetic and Muscular Disorders Research
- Alzheimer's disease research and treatments
San Sebastián University
2023-2024
University of Chile
2009-2023
Center for Climate and Resilience Research
2021-2023
Pontificia Universidad Católica de Chile
2011-2018
Instituto de Ciências Farmacêuticas
2011
Abstract Background In contrast to mammals, amphibians, such as adult urodeles (for example, newts) and anuran larvae Xenopus ) can regenerate their spinal cord after injury. However, the cellular molecular mechanisms involved in this process are still poorly understood. Results Here, we report that tail amputation results a global increase of Sox2 levels proliferation + cells. Overexpression dominant negative form diminished resident cells affecting regeneration amputation, suggesting is...
Xenopus laevis has regenerative and non-regenerative stages. As a tadpole, it is fully capable of functional recovery after spinal cord injury, while its juvenile form (froglet) loses this capability during metamorphosis. We envision that comparative studies between stages in could aid understanding why regeneration fails human beings. To identify the mechanisms allow tadpole to regenerate inhibit froglet, we obtained transcriptome-wide profile response injury found extensive transcriptome...
The capacity to regenerate the spinal cord after an injury is a coveted trait that only limited group of nonmammalian organisms can achieve. In Xenopus laevis, this present during larval or tadpole stages, but absent postmetamorphic frog stages. This provides excellent model for comparative studies between regenerative and nonregenerative stage identify cellular molecular mechanisms explain difference in potential. Here, we used iTRAQ chemistry obtain quantitative proteome 1 day transection...
Dysregulated central-energy metabolism is a hallmark of brain aging. Supplying enough energy for neurotransmission relies on the neuron-astrocyte metabolic network. To identify genes contributing to age-associated functional decline, we formulated an approach analyze network by integrating flux, structure and transcriptomic databases Our findings support that during aging: (1) The astrocyte undergoes switch from aerobic glycolysis oxidative phosphorylation, decreasing lactate supply neuron,...
Transposable elements comprise a large proportion of animal genomes. Transposons can have detrimental effects on genome stability but also offer positive roles for evolution and gene expression regulation. Proper balance the deleterious transposons is crucial cell homeostasis requires mechanism that tightly regulates their expression. Herein we describe DNA Tc1/mariner superfamily during Xenopus development. Sense antisense transcripts containing complete Tc1-2_Xt were detected in embryos....
The transcriptome analysis of injured Xenopus laevis tadpole and mice suggested that Neurod4L.S., a basic-helix-loop-helix transcription factor, was the most promising factor to exert neuroregeneration after spinal cord injury (SCI) in mammals. We generated pseudotyped retroviral vector with neurotropic lymphocytic choriomeningitis virus (LCMV) envelope deliver murine Neurod4 undergoing SCI. SCI induced ependymal cells neural stem (NSCs) central canal. LCMV envelope-based pseudotypedvector...
Classical Rett syndrome (RTT) is a neurodevelopmental disorder caused by mutations in the MECP2 gene, resulting devastating phenotype associated with lack of gene expression control. Mouse models lacking Mecp2 an RTT-like have been developed to advance therapeutic alternatives. Environmental enrichment (EE) attenuates RTT symptoms patients and mouse models. However, mechanisms underlying effects EE on not fully elucidated. We housed male hemizygous Mecp2-null (Mecp2-/y) wild-type mice...
Transposable elements (retrotransposons and DNA transposons) comprise a large proportion of animal genomes, for example 20% in D. melanogaster, 36% X. tropicalis 45% humans. After invading new genome, the transposable element increases its copy number subsequently accumulates mutations. These may eventually result inactive copies. Until recent days transposons have been considered "junk" no clear function assigned this important amount information on genomes.
SUMMARY Dysregulated central-energy metabolism is a hallmark of brain aging. Supplying enough energy for neurotransmission relies on the neuron-astrocyte metabolic network. To identify genes contributing to age-associated functional decline, we formulated an approach analyze network by integrating flux, structure and transcriptomic databases Our findings support that during aging: 1) The astrocyte undergoes switch from aerobic glycolysis oxidative phosphorylation, decreasing lactate supply...
Relatively few studies have addressed how aging contributes to late-onset Alzheimer's disease (LOAD) development and the mechanisms remain largely unknown. Here authors integrate gene expression data from ∼1000 brains, comparing normal with LOAD individuals across different brain regions identify aging-specific LOAD-specific signatures. They also a subgroup of normal-aging samples highly similar LOAD, representing high risk for developing LOAD. This high-risk signature contains excellent...
While the role of lactate shuttle from glia to neurons in central nervous system has been associated with neurodegenerative disease, this Schwann cells peripheral during nerve degeneration remains unknown. Here authors report that deletion dehydrogenase, enzyme catalyzes interconversion between pyruvate and motor or cells, causes progressive neuron axon degeneration. However, sensory remain unaffected. The dehydrogenase presents a novel target for rescuing metabolic dysfunction...