A5060162713- Antifungal resistance and susceptibility
- Advanced biosensing and bioanalysis techniques
- Fungal Infections and Studies
- DNA and Nucleic Acid Chemistry
- Fungal Biology and Applications
- Telomeres, Telomerase, and Senescence
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- Synthesis and Biological Evaluation
- Plant Pathogens and Fungal Diseases
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Research on Leishmaniasis Studies
- Synthesis and biological activity
- RNA Research and Splicing
- Quinazolinone synthesis and applications
- Fungal Plant Pathogen Control
- Microbial Natural Products and Biosynthesis
- CRISPR and Genetic Engineering
- Helminth infection and control
- Carbohydrate Chemistry and Synthesis
- Trypanosoma species research and implications
- Pneumocystis jirovecii pneumonia detection and treatment
- Cancer Mechanisms and Therapy
- Phytochemical compounds biological activities
Cibles et Médicaments des Infections et de l'Immunité
2012-2024
Nantes Université
2018-2024
Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques
2005-2020
Sorbonne Université
2009-2017
Centre National de la Recherche Scientifique
2009-2017
Institut Curie
2009-2017
Université Paris Cité
2005-2010
Hospital Universitario Dr José Eleuterio Gonzalez
2005
Virginia Commonwealth University
2005
Universidad Autónoma de Nuevo León
2005
ABSTRACT Candida parapsilosis has recently emerged as a major threat due to the worldwide emergence of fluconazole-resistant strains causing clonal outbreaks in hospitals and poses therapeutic challenge limited antifungal armamentarium. Here, we used precise genome editing using CRISPR-Cas9 gain further insights into contribution mutations ERG11 , ERG3 MRR1 TAC1 genes influence allelic dosage resistance C. . Seven most common amino acid substitutions previously reported clinical isolates...
(2-(2,4-Dichlorophenyl)-3-(1H-indol-1-yl)-1-(1,2,4-1H-triazol-1-yl)propan-2-ol (8 g), a new 1,2,4-triazole-indole hybrid molecule, showed broad-spectrum activity against Candida, particularly low fluconazole-susceptible species. Its was higher than fluconazole and similar to voriconazole on C. glabrata (MIC90 = 0.25, 64 1 µg/mL, respectively), krusei 0.125, 0.125 respectively) albicans 0.5, 8 0.25 respectively). The action mechanisms of g were also identified as inhibition ergosterol...
Mechanisms of telomere replication remain poorly defined. It has been suggested that G-rich telomeric strand by lagging mechanisms requires, in a stochastic way, the WRN protein. Here we show this requirement is more systematic than previously thought. Our data are compatible with situation which, absence WRN, DNA synthesis at forks uncoupled, thus allowing to continue on C strand, while single G strands accumulate. We also cells which both and POT1 limiting, G- C-rich shorten, suggesting...
The folding of AG3(T2AG3)3 was investigated in the presence Na+ or K+ ions, by using dinuclear platinum complexes [{trans-PtCl(NH3)2}2H2N(CH2)nNH2]Cl2 (n = 2 6). has been previously found to adopt two different quadruplex structures: antiparallel one a solution containing and parallel K+-containing crystal. structures are strikingly distinct not expected form same cross-links. Therefore, characterization cross-links formed with allowed predominant conformation(s) be identified. bases...
Background Gene amplification is thought to promote over-expression of genes favouring tumour development. Because amplified regions are usually megabase-long, often concerns numerous syntenic or non-syntenic genes, among which only a subset over-expressed. The rationale for these differences remains poorly understood. Methodology/Principal Finding To address this question, we used quantitative RT-PCR determine the expression level series co-amplified in five xenografted and one fresh human...
Sterols are the main components of fungal membranes. Inhibiting their biosynthesis is mode action azole antifungal drugs that widely used to treat disease including aspergillosis. Azole resistance has emerged as a matter concern but little known about sterols in resistant Aspergillus fumigatus.We explored sterol composition 12 A. fumigatus isolates, nine isolates with TR34/L98H, TR46/Y121F/T289A or TR53 alterations cyp51A gene and its promoter conferring resistance. Modifications were also...
In this review, we discuss novel natural products discovered within the last decade that are reported to have antifungal activity against pathogenic species. Nearly a hundred were identified originate from bacteria, alga, fungi, sponges and plants. Fungi most prolific source of compounds during period review. The structural diversity these leads encompasses all major classes including polyketides, shikimate metabolites, terpenoids, alkaloids peptides.
Aim: To evaluate antifungal potential of 5,6,7,8-tetrahydroimidazo[1,2-a]pyrazine hybrids based on thiosemicarbazones and thiazolidinediones against pathogenic Sporothrix species. Methods: Antifungal activity nine compounds were assessed by broth microdilution. Interactions between active itraconazole evaluated the checkerboard assay using non-wild-type isolates. Cytotoxicity was determined. Results: Four C-3 substituted analogs showed activity, unrelated to thiosemicarbazone or...
Pim kinases (proviral integration site for Moloney murine leukemia virus kinases) are overexpressed in various types of hematological malignancies and solid carcinomas, promote cell proliferation survival. Thus, validated as targets antitumor therapy. In this context, our combined efforts natural product-inspired library generation screening furnished very promising dibenzo[b,d]furan derivatives derived from cercosporamide. Among them, lead compound 44 was highlighted a potent Pim-1/2...