A5045820806- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Synthesis and biological activity
- Microtubule and mitosis dynamics
- Cell death mechanisms and regulation
- Quinazolinone synthesis and applications
- Synthesis and Characterization of Heterocyclic Compounds
- Cancer-related Molecular Pathways
- Synthesis and Reactivity of Heterocycles
- DNA Repair Mechanisms
- Click Chemistry and Applications
- RNA Interference and Gene Delivery
- Synthesis and Biological Evaluation
- Cancer Mechanisms and Therapy
- Genomics and Chromatin Dynamics
- Retinoids in leukemia and cellular processes
- Cancer therapeutics and mechanisms
- Bioactive Compounds and Antitumor Agents
- Chronic Lymphocytic Leukemia Research
- Ubiquitin and proteasome pathways
- Microbial Natural Products and Biosynthesis
- Synthesis of Organic Compounds
- Protein Tyrosine Phosphatases
- PARP inhibition in cancer therapy
- Plant nutrient uptake and metabolism
Station Biologique de Roscoff
2016-2025
Laboratoire de Biologie Intégrative des Modèles Marins
2014-2025
Sorbonne Université
2016-2025
Centre National de la Recherche Scientifique
2016-2025
University of Edinburgh
2000-2019
Wellcome Centre for Cell Biology
2002-2019
MRC Protein Phosphorylation and Ubiquitylation Unit
2016
Institute of Structural and Molecular Biology
2009
University College London
2009
Edinburgh College
2000
The chromosomal passenger complex of Aurora B kinase, INCENP, and Survivin has essential regulatory roles at centromeres the central spindle in mitosis. Here, we describe Borealin, a novel member complex. Approximately half mitotic cells is complexed with Survivin; Borealin binds INCENP vitro. A second contains but no or Survivin. Depletion by RNA interference delays progression results kinetochore–spindle misattachments an increase bipolar spindles associated ectopic asters. extra poles,...
Survivin is a key cellular protein thought to function in apoptotic regulation, mitotic progression, or possibly both. In this study, we describe the isolation of two conditional knockouts survivin gene chicken DT40 cells. cells lacking die interphase after failing complete cytokinesis. However, these show normal sensitivity chemotherapeutic agent etoposide. Expression mutants against null background reassess role several residues reveals that can grow normally if their sole missing widely...
In t(15;17) acute promyelocytic leukemia, all-trans retinoic acid (RA) induces leukemic cell maturation in vitro and remission leukemia patients, but vivo treatments invariably lead to relapse with resistance RA. NB4, a maturation-inducible line, NB4-RAr sublines (R1 R2) displaying no the presence of RA have been isolated from patient relapse. We show that is not mere unresponsiveness RA: rather, R1 "resistant" cells do respond (1 microM) by sustained growth, become competent undergo...
ABSTRACT ND10, otherwise known as nuclear dots, PML bodies or PODs, are punctate foci in interphase nuclei that contain several cellular proteins. The functions of ND10 have not been well defined, but they sensitive to external stimuli such stress and virus infection, disrupted malignant promyelocytic leukaemia cells. Herpes simplex type 1 regulatory protein Vmw110 induces the proteasome-dependent degradation component proteins Sp100, particularly species these which covalently conjugated...
Dynamic localization of the chromosomal passenger complex (CPC) during mitosis is essential for its diverse functions. CPC targeting to centromeres involves interactions between Survivin, Borealin, and inner centromere protein (CENP [INCENP]) N terminus. In this study, we investigate how INCENP C terminus aurora B set level kinase activity. Low levels activity, seen in INCENP-depleted cells or expressing a mutant that cannot bind B, are sufficient spindle checkpoint response when...
Aurora kinases are central regulators of mitotic-spindle assembly, chromosome segregation and cytokinesis. B is a member the chromosomal passenger complex (CPC) with crucial functions in regulation attachment kinetochores to microtubules We report here that contains conserved SUMO modification motif within its kinase domain. can bind peptides vitro when bound IN-box domain CPC partner INCENP. Mutation Lys207 arginine (Aurora BK207R) impairs formation conjugates vivo. Expression SUMO-null...
ABSTRACT The protein phosphatase inhibitors okadaic acid and calyculin A at moderate concentrations induced three types of apoptotic promyelocytic leukemia cell death, distinct with respect to ultrastructure polynucleotide fragmentation. Calyculin higher (>50 nM) a non-apoptotic death type high ATP pronounced micromitochondriosis. This suggests that phosphorylation pathways are involved in the triggering several pathways. Activation cAMP kinase yet another type, preferentially...
LIM Kinases, LIMK1 and LIMK2, have become promising targets for the development of inhibitors with potential application treatment several major diseases. LIMKs play crucial roles in cytoskeleton remodeling as downstream effectors small G proteins Rho-GTPase family, regulators cofilin, an actin depolymerizing factor. In this article we describe conception, synthesis, biological evaluation novel tetrahydropyridine pyrrolopyrimidine LIMK inhibitors. Homology models were first constructed to...
MCF-7 human breast cancer cells are widely utilized to study apoptotic processes. Recent studies demonstrated that these lack procaspase-3. In the present study, caspase activation and activity were examined in this cell line after treatment with microtubule poison paclitaxel. When harvested 72 h start of a 24-h 100 nmpaclitaxel, 37 ± 5% nonadherent displayed morphological changes. Although mitochondrial cytochrome<i>c</i> release caspase-9 cleavage detectable by immunoblotting, assays...
Various aromatic ketones were first functionalized next to the carbonyl function by deprotolithiation in presence of a zinc salt followed iodolysis. The outcome reactions was analyzed, and particular their regioselectivity light calculated p K values. halogenated involved copper‐catalyzed twofold C–N bond formation obtain fused systems based on 2‐aminopyrimidines. Besides potential antibacterial effect, 2‐aminobenzothiopyrano[4,3,2‐ de ]quinazoline shown inhibit PIM1 (IC 50 : 0.61 µ m )...