A5006261578- Diabetes Treatment and Management
- Cardiovascular Function and Risk Factors
- Cardiovascular Disease and Adiposity
- Extracellular vesicles in disease
- Cardiac Ischemia and Reperfusion
- Cardiac, Anesthesia and Surgical Outcomes
- Pancreatic function and diabetes
- Cardiovascular Effects of Exercise
- Peptidase Inhibition and Analysis
- Calpain Protease Function and Regulation
- Receptor Mechanisms and Signaling
- Nitric Oxide and Endothelin Effects
- Cardiac and Coronary Surgery Techniques
- Cardiac Fibrosis and Remodeling
- Adenosine and Purinergic Signaling
- Adipose Tissue and Metabolism
- Heart Failure Treatment and Management
- Atherosclerosis and Cardiovascular Diseases
- Cardiac Imaging and Diagnostics
- Metabolism, Diabetes, and Cancer
- Pulmonary Hypertension Research and Treatments
- Cardiac Health and Mental Health
- Tissue Engineering and Regenerative Medicine
- Apelin-related biomedical research
- Neuropeptides and Animal Physiology
Brown University
2016-2025
Rhode Island Hospital
2016-2024
Beth Israel Deaconess Medical Center
2023-2024
Harvard University
2016-2024
Providence College
2020-2023
Lifespan
2023
Cardiovascular Research Center
2022
John Brown University
2022
Cleveland Clinic
2014-2018
Tokushima University Hospital
2016
Background Sodium-glucose cotransporter-2 inhibitors are cardioprotective independent of glucose control, as demonstrated in animal models acute myocardial ischemia and clinical trials. The functional molecular mechanisms these benefits the setting chronic poorly defined. purpose this study is to determine effects canagliflozin therapy on perfusion, fibrosis, function a large model ischemia. Methods Results Yorkshire swine underwent placement an ameroid constrictor left circumflex artery...
Decreased right ventricular (RV) ejection fraction (RVEF) portends poor prognosis in patients with ischemic cardiomyopathy, and previous studies have suggested an association between mitral regurgitation (MR) RVEF. We sought to evaluate this whether valve repair or replacement affects the relationship RV function mortality.We included 588 (mean age, 63±11 years; 75% male) cardiomyopathy who underwent cardiac magnetic resonance imaging 2002 2008. Baseline characteristics, left fraction, MR...
ObjectiveLimited treatments exist for nonoperative chronic coronary artery disease. Previously, our laboratory has investigated extracellular vesicle (EV) therapy as a potential treatment disease using swine model and demonstrated improved cardiac function in treated with intramyocardial EV injection. Here, we seek to investigate the benefits of EVs by hypoxia-conditioned (HEV). Specifically, this study aims effect HEV on apoptosis chronically ischemic myocardium swine.MethodsFourteen...
The past several decades have borne witness to breakthroughs and paradigm shifts within the field of cardiovascular medicine, but one component that has remained constant throughout this time is need for accurate animal models refinement elaboration hypotheses therapies crucial our capacity combat human disease. Numerous sophisticated high-throughput molecular strategies emerged, including rational drug design multi-omics approaches allow extensive characterization host response disease...
There is a significant body of literature to suggest that coronary artery disease (CAD) highly sex-specific disease. The study therapeutics and responses treatment for CAD remains underreported in the literature. Sodium-glucose transporter 2 (SGLT2) inhibitors are growing interest ischemic heart failure; however, response SGLT2 unknown. We studied an inhibitor, canagliflozin, swine model metabolic syndrome (MS) chronic myocardial ischemia with emphasis on outcomes. Yorkshire (n = 21) were...
Abstract In animal models, human bone marrow mesenchymal stem cell‐derived extracellular vesicles (MSC‐EV) have been found to beneficial effects in cardiovascular disease, but only when administered via intramyocardial injection. The biodistribution of either intravenous or injection MSC‐EV the presence myocardial injury is uncharacterized at this time. We hypothesized that will ensure delivery ischemic myocardium, while not. Human cells were cultured and isolated characterized. MSC‐EVs then...
We have previously shown that normoxia serum-starved extracellular vesicle (EV) therapy improves myocardial function, perfusion, and angiogenesis in a swine model of chronic ischemia. Hypoxia-modified EVs increased abundance anti-oxidant, pro-angiogenic, pro-survival proteins. The purpose this study is to investigate the differential effects hypoxia-modified on microvascular density chronically ischemic myocardium. Yorkshire underwent placement an ameroid constrictor left circumflex artery...
Introduction: Patients with advanced coronary artery disease (CAD) who are not eligible for stenting or surgical bypass procedures have limited treatment options. Extracellular vesicles (EVs) emerged as a potential therapeutic target the of CAD. These EVs can be conditioned to modify their contents. In our previous research, we demonstrated increased perfusion, decreased inflammation, and reduced apoptosis intramyocardial injection hypoxia-conditioned (HEVs). The goal this study is further...
Despite the overwhelming use of cellularized therapeutics in cardiac regenerative engineering, approaches to biomanufacture engineered tissues (ECTs) at clinical scale remain limited. This study aims evaluate impact critical biomanufacturing decisions—namely cell dose, hydrogel composition, and size-on ECT formation function—through lens translation. ECTs were fabricated by mixing human induced pluripotent stem-cell-derived cardiomyocytes (hiPSC-CMs) fibroblasts into a collagen engineer...
Abstract Obesity continues to rise in the juveniles and obese children are more likely develop metabolic syndrome (MetS) related cardiovascular disease. Unfortunately, effective prevention long‐term treatment options remain limited. We determined juvenile cardiac response MetS a swine model. Juvenile male were fed either an obesogenic diet, induce MetS, or lean as control (LD). Myocardial ischemia was induced with surgically placed ameroid constrictor on left circumflex artery. Physiological...
Small conductance calcium-activated potassium (SK) channels are largely responsible for endothelium-dependent coronary arteriolar relaxation. Endothelial SK downregulated by the reduced form of nicotinamide adenine dinucleotide (NADH), which is increased in setting diabetes, yet mechanisms these changes unclear. PKC (protein kinase C) an important mediator diabetes-induced endothelial dysfunction. Thus, we aimed to determine whether NADH signaling downregulates channel function via PKC....
Background: Sodium–glucose cotransporter-2 (SGLT2) inhibitors are known to be cardioprotective independent of glucose control, but the mechanisms these benefits unclear. We previously demonstrated improved cardiac function and decreased fibrosis in a swine model chronic myocardial ischemia. The goal this study is use high-sensitivity proteomic analyses characterize specific molecular pathways affected by SGLT-2 inhibitor canagliflozin (CAN) therapy Methods: Chronic ischemia was induced...
Abstract Small animal models have shown improved cardiac function with DPP‐4 inhibition, but many human studies worse outcomes or no benefit. We seek to bridge the gap by studying inhibitor sitagliptin in a swine model of chronic myocardial ischemia using proteomic analysis. Thirteen Yorkshire underwent placement an ameroid constrictor on left coronary circumflex artery ischemia. Two weeks post‐op, received either 100 mg daily (SIT, n = 5) drug (CON, 8). After 5 treatment, functional...
Abstract Dipeptidyl peptidase 4 inhibitors (DPP4i) may be cardioprotective based on several small animal and clinical studies, though randomized control trials have demonstrated limited benefit. Given these discrepant findings, the role of agents in chronic myocardial disease, particularly absence diabetes, is still poorly understood. The purpose this study was to determine effects sitagliptin, a DPP4i, perfusion microvessel density clinically relevant large model ischemia. Normoglycemic...