A5036257319- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Parasites and Host Interactions
- Insect and Pesticide Research
- Mosquito-borne diseases and control
- Autoimmune and Inflammatory Disorders Research
- Hepatitis Viruses Studies and Epidemiology
- Viral Infections and Vectors
- Eosinophilic Disorders and Syndromes
- Malaria Research and Control
- Toxin Mechanisms and Immunotoxins
- Viral gastroenteritis research and epidemiology
- Insect Pest Control Strategies
- CRISPR and Genetic Engineering
Umeå University
2021-2024
Banaras Hindu University
2011-2023
Institute of Medical Sciences
2011-2023
BackgroundVisceral leishmaniasis, also known on the Indian subcontinent as kala-azar, is a fatal form of leishmaniasis caused by protozoan parasite Leishmania donovani and transmitted bites vector sandfly Phlebotomus argentipes. To achieve sustain elimination visceral transmission potential individuals exposed to L from across infection spectrum needs be elucidated. The aim this study was evaluate relative infectiousness patients with or post-kala-azar dermal before after treatment,...
PCR based diagnosis for Visceral Leishmaniasis (VL), despite numerous published primers, remains far from being applied in the field. The present study was planned to design a Leishmania specific diagnostic assay and evaluate its sensitivity specificity on sample size, which best of our knowledge is largest ever screened one study.Leishmania primers were developed using 18S rRNA gene their evaluated 500 parasitologically confirmed patients with VL 25 Post Kala-azar Dermal (PKDL) patients....
Background Visceral Leishmaniasis (VL) is a life threatening neglected infectious disease in the Indian subcontinent, transmitted by bite of female sand flies. Estimation infectivity vector population, collected different seasons, may be useful to better understanding transmission dynamics VL as well plan control measures. Methodology We flies from highly endemic regions Bihar state, India for one year over three seasons. The species were confirmed species-specific PCR-RFLP. Leishmania...
Abstract Objectives Recent epidemiological reports indicate that asymptomatic human infections with Leishmania donovani , the causative agent of visceral leishmaniasis or Kala‐azar ( KA ), occur frequently in India. We explored markers infection. Methods Blood samples were collected from 286 healthy subjects 16 villages Muzaffarpur district Bihar. These individuals classified into three groups: (i) persons no history and living a house where cases previously reported, (ii) but diagnosed at...
Visceral leishmaniasis (VL) is a vector-borne protozoan disease, which can be fatal if left untreated. Synthetic chemical insecticides are very effective tools for controlling of insect vectors, including the sand fly Phlebotomus argentipes , vector VL in Indian subcontinent. However, repeated use same insecticide with increasing doses potentially create high selection pressure and lead to tolerance resistance development. The objective this study was determine lethal concentrations assess...
Abstract Background SLC11A1 has pleiotropic effects on macrophage function and remains a strong candidate for infectious disease susceptibility. 5' and/or 3' polymorphisms have been associated with tuberculosis, leprosy, visceral leishmaniasis (VL). Most studies undertaken to date were under-powered, none replicated within population. Association tuberculosis variably across populations. Here we investigate VL in India. Methods Nine (rs34448891, rs7573065, rs2276631, rs3731865, rs17221959,...
Abstract Background IL8RA and IL8RB, encoded by CXCR1 CXCR2 , are receptors for interleukin (IL)-8 other CXC chemokines involved in chemotaxis activation of polymorphonuclear neutrophils (PMN). Variants at have been associated with susceptibility to cutaneous mucocutaneous leishmaniasis Brazil. Here we investigate the role CXCR1/CXCR2 visceral (VL) India. Methods Three single nucleotide polymorphisms (SNPs) (rs4674259, rs2234671, rs3138060) that tag linkage disequilibrium blocks across were...
Leishmaniasis is a vector-borne disease, and in the Indian subcontinent female Phlebotomus argentipes vector for Leishmania donovani. However, data on extent of sand fly infection rates natural settings using molecular methods have not been extensively reported India. In this study PCR technique was applied targeting 18S rRNA encoding region to determine prevalence P. captured field. For study, flies were collected from 897 houses selected 50 villages endemic visceral leishmaniasis (VL)...
This pilot project was preliminary and essential to a larger effort define the ability of certain human-subject groups across infection spectrum serve as reservoirs Leishmania donovani sand flies in areas anthroponotic transmission such Bihar state, India. is possible only via xenodiagnosis well-defined subject using live vector flies. The objective establish at Kala Azar Medical Research Center (KAMRC), Muzaffarpur, Bihar, India, self-sustaining colony Phlebotomus argentipes (Annandale &...
Journal Article PCR-RFLP Based Method for Molecular Differentiation of Sand Fly Species Phlebotomus argentipes, papatasi, and Sergentomyia babu Found in India Get access Puja Tiwary, Tiwary Infectious Disease Research Laboratory, Department Medicine, Institute Medical Sciences, Banaras Hindu University, Varanasi 221 005, U.P., Search other works by this author on: Oxford Academic PubMed Google Scholar Dinesh Kumar, Kumar Madhukar Rai, Rai Shyam Sundar 1 1Corresponding author, e-mail:...
Interleukin-18 (IL-18) is a cytokine that mediates Th1 response by inducing interferon-gamma (IFN- γ ) production in T cells and natural killer cells. Genetic polymorphisms the IL-18 gene have been found to be associated with its expression cancer, tuberculosis, HBV infection, various other diseases. Lower plasma level of visceral leishmaniasis (VL) patients might regulating or coding region gene. Three single nucleotide (SNPs), rs1946519 (−656 G/T) rs187238 (−137 G/C) promoter rs549908...
Leishmaniasis on the Indian subcontinent is thought to have an anthroponotic transmission cycle. There no direct evidence that a mammalian host other than humans can be infected with Leishmania donovani and transmit infection sand fly vector. The aim of present study was evaluate impact feeding domestic species provide clinical regarding possible non-human reservoirs through experimental cows, water buffalo goats rodents. We performed xenodiagnosis using colonized Phlebotomus argentipes...
SUMMARY Genetic screens with barcoded Plasmo GEM vectors have identified thousands of Plasmodium gene functions in haploid blood stages, gametocytes and liver stages. However, the formation diploid cells by fertilisation has hindered use genetic to investigate vector-parasite interactions during mosquito stages parasite. In this study, we developed a scalable system that uses targeting equipped CRISPR-mediated homing mechanism generate homozygous loss-of-function mutants reveal functionally...
Rapid diagnostic tests (RDTs) based on the detection of specific antibodies in serum are commonly used for diagnosis visceral leishmaniasis (VL). Several commercial kits available, and some them allow use whole-blood samples instead serum. An RDT is much more user-friendly blood than samples. In this study, we examined sensitivities specificities six different commercially available immunochromatographic their accuracy detecting Leishmania infection whole parasitologically confirmed VL...
Miltefosine is the only orally administrable drug for treatment of leishmaniasis. But in recent years, a decline its efficacy points toward emergence resistance to this drug. Knowledge biomarkers miltefosine may be beneficial proper selection regimen. Splenic aspirates were collected and parasites cultured from patients relapsed after initial cure (N = 15) successfully treated with miltefosine. Differential expression genes miltefosine-resistant strains was examined by DNA microarray...
Visceral leishmaniasis derived from Leishmania donovani is transmitted by sand flies (Phlebotomus argentipes) throughout the Indian subcontinent. Although considered anthroponotic, L. infects other mammals susceptible to fly bites, including dogs. Aggressive strategies reduce populations in India have led seeking nonhuman hosts, so understanding role of dogs transmission has become critical. Our study investigated infection and potential for such infections be back flies. We performed...
Background: Blood meal identification of hematophagous insects provides information on host-feeding preferences in natural conditions. In the present study, we performed blood analysis among blood-fed female Phlebotomus argentipes collected from VL endemic regions Muzaffarpur district, Bihar, India Methods & Materials: Sand flies were by light traps and processed individually for DNA isolation further PCR RFLP. To discriminate animal host using meals, a region mitochondrial DNA, cytB gene...
Background: Interleukin 18 (IL-18) is a cytokine that mediates Th1 response by inducing interferon-gamma (IFN-γ) production in T cells and natural killer cells. Genetic polymorphisms the IL-18 gene have been found to be associated with its expression cancer, tuberculosis, HBV infection various other diseases. Lower plasma level of visceral leishmaniasis patients might coding gene. Methods & Materials: Three single nucleotide polymorphisms, rs1946519 (-656 G/T) rs187238 (-137 G/C) promoter...
Background: Miltefosine is the only orally administrable drug for treatment of leishmaniasis. It used as a first line Kala-azar Elimination Programme in Indian subcontinent. But recent years, decline its efficacy points toward emergence resistance to this drug. Knowledge biomarkers miltefosine may be beneficial proper selection regimen. Microarray-based gene expression profile relapsed parasites over sensitive strains Differential A. Calpain family cysteine protease-like protein and B....
Miltefosine is the only orally administrable drug for treatment of leishmaniasis. But in recent years, a decline its efficacy points toward emergence resistance to this drug. Knowledge biomarkers miltefosine may be beneficial proper selection regimen. Splenic aspirates were collected and parasites cultured from patients relapsed after initial cure (n=15) successfully treated with miltefosine. Differential expression genes resistant strains, was examined by DNA microarray, validated real time...