A5062216474- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- DNA and Nucleic Acid Chemistry
- Metabolism, Diabetes, and Cancer
- Glycosylation and Glycoproteins Research
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Carcinogens and Genotoxicity Assessment
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Barrier Structure and Function Studies
- Toxin Mechanisms and Immunotoxins
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Ion Transport and Channel Regulation
- Genetic factors in colorectal cancer
- Connexins and lens biology
- Plant Genetic and Mutation Studies
- Mitochondrial Function and Pathology
- Nuclear Materials and Properties
- Drug Transport and Resistance Mechanisms
- Biochemical Analysis and Sensing Techniques
- ATP Synthase and ATPases Research
Ulsan National Institute of Science and Technology
2020-2023
Institute for Basic Science
2020-2023
The University of Texas MD Anderson Cancer Center
2009-2019
University of Houston
2014
Genesis Foundation
2003-2011
The University of Texas at Austin
2010
Oregon Health & Science University
2010
Gunma University
1995-2008
Nisshin Steel (Japan)
2008
Tokyo University of Science
1986-2008
Although a defect in the DNA polymerase POLQ leads to ionizing radiation sensitivity mammalian cells, relevant enzymatic pathway has not been identified. Here we define specific mechanism by which restricts harmful instability. Our experiments show that Polq-null murine cells are selectively hypersensitive strand breaking agents, and damage resistance requires activity of POLQ. Using break end joining assay monitored repair ends with long 3' single-stranded overhangs. End events retaining...
To elucidate a functional role of N-glycosylation in glucose transporters, we introduced oligonucleotide-directed mutagenesis GLUT1 cDNA to remove the possible site for N-linked glycosylation. The wild-type and mutated cDNAs which induced mutation Asn at residue 45 Asp, Tyr, or Gln were transfected stably expressed into Chinese hamster ovary cells. was demonstrated be broad band 45-60-kDa form sharp 38-kDa on Western blot analysis, respectively, indicating no glycosylation GLUT1. Although...
Article22 May 2018Open Access Source DataTransparent process FAM35A associates with REV7 and modulates DNA damage responses of normal BRCA1-defective cells Junya Tomida Corresponding Author [email protected] orcid.org/0000-0002-0813-5757 Department Epigenetics & Molecular Carcinogenesis, The University Texas MD Anderson Cancer Center, Smithville, TX, USA Search for more papers by this author Kei-ichi Takata Sarita Bhetawal Maria D Person Proteomics Facility, at Austin, Hsueh-Ping Chao Dean G...
Abstract Mammalian HELQ is a 3′–5′ DNA helicase with strand displacement activity. Here we show that participates in pathway of resistance to interstrand crosslinks (ICLs). Genetic disruption human cells enhances cellular sensitivity and chromosome radial formation by the ICL-inducing agent mitomycin C (MMC). A significant fraction MMC independent Fanconi anaemia pathway. Sister chromatid exchange frequency UV radiation or topoisomerase inhibitors unaltered. Proteomic analysis reveals...
DNA polymerase theta (POLQ)-mediated end joining (TMEJ) is a distinct pathway for mediating double-strand break (DSB) repair. TMEJ required the viability of BRCA-mutated cancer cells. It crucial to identify tumors that rely on POLQ activity DSB repair, because such are defective in other repair pathways and have predicted sensitivity inhibition therapies produce DSBs. We define here POLQ-associated mutation signatures human cancers, characterized by short insertions deletions specific range...
The localization of 2 isoforms glucose transporters (GLUT1 and GLUT4) in 75 patients with renal cell carcinoma was examined immunohistochemically. Paraffin sections were immunostained either anti-GLUT1 or GLUT4 antibody by the avidin-biotin-peroxidase complex method. In 55 patients, GLUT1 staining demonstrated at plasma membrane cancer cells. clear subtype, 44 52 (84.6%) positive for GLUT1. We did not detect spindle type. mixed recognized only areas carcinomas (10 13; 76.9%). Positive show...
Glucose is actively taken up from the glomerular filtrate into tubule cells by Na(+)-dependent active glucose transporter (GT), and passively crosses basolateral membrane via facilitated diffusion GT. With use of antibodies directed against two types GTs, we show immunocytochemical localization GT (SGLT1) erythrocyte/HepG2-type (GLUT1). For light microscopic observation, frozen sections were stained rhodamine labeling method. Counterstaining with fluorescein-phalloidin...
We have used high affinity polyclonal antibodies specific for phosphotyrosine (PTyr) residues to examine the localization in various chick embryonic tissues situ of PTyr-modified proteins by immunocytochemical methods. During period from 9 21 d development, most exhibit elevated levels as determined immunoblotting experiments tissue extracts with anti-PTyr (Maher, P. A., and E. B. Pasquale. 1988. J. Cell Biol. 106:1747-1755). By immunofluorescence labeling semithin frozen sections, highest...
DNA polymerase zeta (pol ) is exceptionally important for controlling mutagenesis and genetic instability.REV3L comprises the catalytic subunit, while REV7 (MAD2L2) considered an accessory subunit.However, it has not been established that role of in damage tolerance necessarily connected with mammalian pol , there accumulating evidence REV3L have independent functions.Analysis hampered by difficulties expression cells, lack a functional complementation system.Here, we report interacts...
Facilitative glucose transporter isoforms, GLUT1 and GLUT4, have different intracellular distributions despite their very similar structure. In insulin-responsive tissues such as adipose muscle, GLUT4 protein resides mainly in the region a basal condition is translocated to plasma membrane upon stimulation of insulin. contrast, was distributed about equally between membranes low density microsomal 3T3-L1 adipocytes. Furthermore, were reported be differentially targeted region, respectively,...
The notion of an insulin-dependent translocation the glucose transporter in rat adipocytes was confirmed by immunoblotting and reconstitution transport activity subcellular fractions. Quantitatively, however, significantly different results were obtained with these two techniques; when compared reconstitution, detected a larger amount from low density microsome fraction to plasma membrane fraction. acidtropic agents chloroquine dibucaine, which have been reported inhibit recycling various...
DNA polymerase ν (POLN or pol ν) is a newly discovered A family that generates high error rate when incorporating nucleotides opposite dG; its translesion synthesis (TLS) capability has only been demonstrated for fidelity replication bypass of thymine glycol lesions. In the current investigation, we describe novel TLS substrate specificity ν, demonstrating it able to exceptionally large lesions whose linkages are through major groove. Specifically, catalyzed efficient and past peptides...
DNA double-strand break (DSB) repair via homologous recombination is initiated by end resection. The extent of resection determines the choice DSB pathway. Nucleases for have been extensively studied. However, it still unclear how potential structures generated initial short MRE11-RAD50-NBS1 are recognized and recruit proteins, such as EXO1, to sites facilitate long-range We found that MSH2-MSH3 mismatch complex recruited through interaction with chromatin remodeling protein SMARCAD1....
G-protein-coupled receptors generally share a similar structure containing seven membrane-spanning domains and extracellular site(s) for N-glycosylation. The histamine H2 receptor is member of the family receptors, has three potential sites N-glycosylation (Asn-4, Asn-162 Asn-168). To date, however, no information been presented regarding receptor. investigate presence, location functional roles receptor, site-directed mutagenesis was performed to eliminate singly collectively. wild-type...
Abstract DNA polymerase θ (POLQ) is a unique that able to perform microhomology-mediated end-joining as well translesion synthesis (TLS) across an abasic (AP) site and thymine glycol (Tg). However, the biological significance of TLS activity currently unknown. Herein we provide evidence POLQ plays critical role in repairing complex double-strand breaks (DSBs) induced by high linear energy transfer (LET) radiation. Radiotherapy with LET radiation such carbon ions leads more deleterious...
The Saccharomyces cerevisiae poly(A) polymerases Trf4 and Trf5 are involved in an RNA quality control mechanism, where polyadenylated RNAs degraded by the nuclear exosome. Although Trf4/5 homologue genes distributed throughout multicellular organisms, their biological roles remain to be elucidated. We isolated here two homologues of Drosophila melanogaster, named DmTRF4-1 DmTRF4-2, investigated function. displayed polymerase activity vitro, whereas DmTRF4-2 did not. Gene knockdown...
To investigate the biological and biochemical properties of GLUT5, rat GLUT5 complementary DNA was transfected into Chinese hamster ovary cells. Rat exclusively targeted to plasma membrane exhibited a transport activity, not for glucose, but fructose. The affinity fructose (Km = 11.6mM) much higher than that GLUT2, other glucose transporter with activity. Interestingly, photolabeled 0.5 microM cytochalasin B, whereas similar amount GLUT1 adequately under same experimental conditions. Next,...
We have expressed GLUT3 protein, an isoform of a facilitative glucose transporter, in Chinese hamster ovary cells by transfection its cDNA using expression vector. The protein was detected Western-blot analysis as broad band 45-65 kDa, indicating intensive glycosylation the protein. observed, immunofluorescence staining, to be located mainly at plasma membrane, and associated with marked increase glucose-transport activity. Kinetic revealed that Km value for 3-O-methylglucose uptake approx....