A5011202744- Birth, Development, and Health
- Diet and metabolism studies
- Pregnancy and preeclampsia studies
- Pancreatic function and diabetes
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Genetics and Neurodevelopmental Disorders
- Digestive system and related health
- Metabolism, Diabetes, and Cancer
- Connexins and lens biology
- Cancer Risks and Factors
- Mitochondrial Function and Pathology
- Barrier Structure and Function Studies
- Regulation of Appetite and Obesity
- Caveolin-1 and cellular processes
- Drug Transport and Resistance Mechanisms
- Dietary Effects on Health
- Neuroscience of respiration and sleep
- Congenital Diaphragmatic Hernia Studies
- Genetic Syndromes and Imprinting
- bioluminescence and chemiluminescence research
- Protein Kinase Regulation and GTPase Signaling
- Neonatal and fetal brain pathology
- Metabolism and Genetic Disorders
- Neuroscience and Neuropharmacology Research
Mattel Children's Hospital
2018-2025
University of California, Los Angeles
2004-2019
Children’s Institute
2019
Ewha Womans University Medical Center
2019
Zero to Three
2015
UCLA Health
2014
Gunma University
1996-1999
In-Q-Tel
1998
GTx (United States)
1998
Umma University
1997
In vivo electro‐transfection efficiency and manner of transferred gene expression were investigated by fluorescence microscopic image analysis. Green fluorescent protein (GFP) was used as the genetic marker. Electroporation carried out on liver live rats use disk electrodes mounted in tips tweezers, which directly pressed onto surface a lobe situ. with eight electric pulses 50 ms duration at V gave good transfection judged induced GFP expression. Bright appeared dots, scattered around area...
Phosphatidylinositol 3-kinase (PI 3-kinase) is stimulated by association with a variety of tyrosine kinase receptors and intracellular tyrosine-phosphorylated substrates. We isolated cDNA that encodes 50-kDa regulatory subunit PI an expression cloning method using 32P-labeled insulin receptor substrate-1 (IRS-1). This protein contains two SH2 domains inter-SH2 domain p85α, but the SH3 bcr homology p85α were replaced unique 6-amino acid sequence. Thus, this appears to be generated alternative...
Glucose transporter isoform-3 (GLUT3) is the trophoblastic facilitative glucose transporter. To investigate role of this isoform in embryonic development, we created a novel GLUT3-null mouse and observed arrested early development loss at neurulation stage when both alleles were mutated. This occurred despite presence other related isoforms, particularly GLUT1. In contrast, single allele was mutated, increased cell apoptosis, adaptive changes subcellular localization GLUT3 GLUT1...
Abstract To determine the basis for perinatal nutritional mismatch causing metabolic dysfunction associated steatotic liver disease (MASLD) and diabetes mellitus, we examined adult phenotype, hepatic transcriptome, pancreatic β-islet function. In prenatal caloric restricted rat with intrauterine growth restriction (IUGR) postnatal exposure to high fat fructose (HFhf) or carbohydrate (RC), investigated male female IUGR-Hfhf IUGR-RC, versus HFhf CON offspring. Males more than females displayed...
Glucose transfer across the placental barrier is crucial for fetal development. To investigate role of glucose transporter isoforms in transplacental glucose, we investigated localization transporters GLUT1 and GLUT3 immunohistochemically rat placenta. In labyrinth, site maternofetal exchange substances, both were present, whereas only was detected junctional region. labyrinthine wall, which lies between maternal circulations, exhibited polarized localization; i.e. it present at plasma...
Intrauterine growth restriction (IUGR) leads to obesity, glucose intolerance, and type 2 diabetes mellitus in the adult. To determine mechanism(s) behind this "metabolic imprinting" phenomenon, we examined effect of total calorie during mid- late gestation modified by postnatal ad libitum access nutrients (CM/SP) or nutrient (SM/SP) vs. alone (SM/CP) on skeletal muscle white adipose tissue (WAT) insulin-responsive transporter isoform (GLUT4) expression translocation. A decline GLUT4 protein...
Insulin receptor substrate (IRS)-2 is structurally and functionally similar to IRS-1. Indeed, stimulation with insulin or insulin-like growth factor I led the rapid tyrosine phosphorylation of both IRS-1 IRS-2, which in turn activated phosphatidylinositol (PI) 3-kinase L6 cells rat skeletal muscle. However, IRS-2 was rapidly dephosphorylated (3–10 min after addition insulin/insulin-like I), whereas continued for at least 60 min. The time courses PI activity associated paralleled these...
Abstract Energy balance is regulated by circulating leptin concentrations and hypothalamic receptor (ObRb) signaling via STAT3 but inhibited SOCS3 PTP1B. Leptin enhances anorexigenic neuropeptides (POMC, MC3‐R, MC4‐R) activation while suppressing orexigenic (NPY, AgRP). We investigated in a sex‐specific manner the early (PN2) late (PN21) postnatal mechanisms response to intrauterine (IUGR), (PNGR), combined (IPGR) calorie growth restriction. At PN2, both male female IUGR were hypoleptinemic,...
Postnatal ad libitum caloric intake superimposed on intrauterine growth restriction (IUGR) is associated with adult-onset obesity, insulin resistance, and type 2 diabetes mellitus (T2DM). We hypothesized that this paradigm of prenatal nutrient deprivation–induced programming can be reversed the introduction early postnatal calorie restriction. Ten-month-old male rats exposed to either (IUGR), pre- (IPGR), or limited suckling phase (50% from [PN]1 PN21) (PNGR) were compared age-matched...
Serotonin (5-HT) is critical for neurodevelopment and the serotonin transporter (SERT) modulates levels. Perturbed prenatal postnatal dietary exposures affect developing offspring predisposing to neurobehavioral disorders in adult. We hypothesized that brain 5-HT-SERT imbalance associated with gut dysbiosis forms contributing gut-brain axis dependent mechanism responsible such ultimate phenotypes.
Air pollution (AP) is detrimental to pregnancies including increasing risk factors of gestational diabetes mellitus. We hypothesized that exposure AP causes cardiovascular and metabolic disruption thereby altering placental gene expression, which in turn affects the phenotype embryonic/fetal development. To test this hypothesis, we investigated impact intra-nasal instilled upon day 16-19 maternal mouse status, nutrient transporters, placental-fetal size morphology. further unravel...
Abstract We examined the subcellular distribution of a novel glucose transporter isoform (GLUT8) in murine N2A neuroblastoma cells. Exogenous expression GLUT8‐green fluorescent protein (GFP) DNA constructs mimicked endogenous GLUT8 localization to intracellular vesicles and minimally Giantin‐positive Golgi. This was unlike distributions GLUT1 GLUT3 (predominant neuronal isoform), which were limited predominantly plasma membrane minimal cytoplasm. Although GLUT4‐GFP (insulin responsive...
Fetal nutrient and growth restriction is associated with development of type 2 diabetes. Although the exact mechanisms responsible for this association remain debated, intrauterine and/or postnatal maldevelopment β-cell mass has been proposed as a potential mechanism. To address hypothesis, turnover was assessed in rats exposed to either caloric/growth restriction. In total, four groups male female Sprague Dawley (n = 69) were developed studied: 1) control rats, i.e. mothers rearing pups; 2)...
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The Placenta plays a pivotal role in the maternofetal exchange of substances. In chorioallantoic placentas rodents, maternal and fetal bloods come close labyrinth. Hemotrichorial placenta is formed most rodents including rat, mouse, hamster. Three trophoblast layers composed single cytotrophoblast layer two syncytiotrophoblast endothelium capillaries lie between circulations labyrinthine wall. Many gap junctions are present layers. double-syncytiotrophoblast connected by serve as structural...