A5042135510- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Advanced DC-DC Converters
- Genomics and Phylogenetic Studies
- Microgrid Control and Optimization
- Metabolomics and Mass Spectrometry Studies
- Glycosylation and Glycoproteins Research
- Multilevel Inverters and Converters
- Identification and Quantification in Food
- Machine Learning in Bioinformatics
- Wireless Power Transfer Systems
- Bioinformatics and Genomic Networks
- Electromagnetic Compatibility and Noise Suppression
- Advanced Battery Technologies Research
- Biotin and Related Studies
- Machine Learning and Data Classification
- Galectins and Cancer Biology
- Low-power high-performance VLSI design
- Analytical Chemistry and Chromatography
- Scheduling and Optimization Algorithms
- Fault Detection and Control Systems
- Spectroscopy and Chemometric Analyses
- Assembly Line Balancing Optimization
- Silicon Carbide Semiconductor Technologies
- Acoustic Wave Resonator Technologies
Tsinghua University
2022-2024
Shanghai Jiao Tong University
2021
Beijing Institute of Technology
2016
Institute of Computing Technology
2007-2014
Chinese Academy of Sciences
2007-2014
University of Shanghai for Science and Technology
2012
National Institute of Biological Sciences, Beijing
2010
University of Chinese Academy of Sciences
2007-2010
Abstract This paper describes the pFind 2.0 software package for peptide and protein identification via tandem mass spectrometry. Firstly, most important feature of is that it offers a modularized customized platform third parties to test compare their algorithms. The developers can create own modules following open application programming interface (API) standards then add into workflows in place default modules. In addition, accommodate different requirements, provides four automated...
De novo peptide sequencing has improved remarkably in the past decade as a result of better instruments and computational algorithms. However, de can correctly interpret only approximately 30% high- medium-quality spectra generated by collision-induced dissociation (CID), which is much less than database search. This mainly due to incomplete fragmentation overlap different ion series CID spectra. In this study, we show that higher-energy collisional (HCD) great help because it produces high...
In relative protein abundance determination from peptide intensities recorded in full mass scans, a major complication that affects quantitation accuracy is signal interference coeluting ions of similar m/z values. Here, we present pQuant, software tool solves this problem. pQuant detects signals, identifies for each pair least interfered isotopic chromatograms: one the light and heavy isotope-labeled peptide. On basis these pairs, calculates heavy/light ratios along with their 99.75%...
Core fucosylation (CF) patterns of some glycoproteins are more sensitive and specific than evaluation their total respective protein levels for diagnosis many diseases, such as cancers. Global profiling quantitative characterization CF may reveal potent biomarkers clinical applications. However, current techniques unable to precisely on a large scale. Here we developed robust strategy that integrates molecular weight cutoff, neutral loss-dependent MS(3), database-independent candidate...
Abstract Determining the monoisotopic peak of a precursor is first step in interpreting mass spectra, which basic but non‐trivial. The reason that isolation window precursor, other peaks interfere with determination peak, leading to wrong mass‐to‐charge ratio or charge state. Here we propose method, named pParse, export most probable for precursors, including co‐eluted precursors. We use relationship between position highest and detect candidate clusters. Then, extract three features sort...
Database searching is the technique of choice for shotgun proteomics, and to date much research effort has been spent on improving its effectiveness. However, database faces a serious challenge efficiency, considering large numbers mass spectra ever fast increase in peptide databases resulting from genome translations, enzymatic digestions, post-translational modifications. In this study, we conducted systematic speeding up search engines protein identification illustrate key points with...
In recent years, electron transfer dissociation (ETD) has enjoyed widespread applications from sequencing of peptides with or without post-translational modifications to top-down analysis intact proteins. However, peptide identification rates ETD spectra compare poorly those collision induced (CID) spectra, especially for doubly charged precursors. This is in part due an insufficient understanding the characteristics and consequently a failure database search engines make use rich...
The <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">CLLC</i> converter is a promising topology for bidirectional power conversion applications, such as vehicle-to-grid and battery energy storage systems. In these wide output voltage range needed. However, traditional pulse-frequency modulation method not suitable because of switching frequency range. Inner phase shift (IPS) control good solution this issue. converters, to further increase the...
Sensorless synchronous rectification (SR) is often used in CLLC converters to achieve higher efficiency without extra system cost. Conventionally, sensorless SR methods turn <sc xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">on</small> secondary-side switches at the same instant as primary side. In such conditions, usually operate under hard turn- conditions due existence of output capacitors <inline-formula...
Peptide identification via tandem mass spectrometry is the basic task of current proteomics research. Due to complexity spectra, majority spectra cannot be interpreted at present. The existence unexpected or unknown protein post-translational modifications a major reason. This paper describes an efficient and sequence database-independent approach detecting abundant in high-accuracy peptide spectra. based on observation that modified its unmodified counterpart are correlated with each other...
Intercell moves are caused by exceptional parts which need to be processed in multiple cells. cooperation disrupts the cellular manufacturing philosophy of creating independent cells, but is essential lower costs for enterprises. This article addresses an intercell scheduling problem considering limited transportation capability. To solve this problem, a two-stage ant colony optimization approach proposed, pre-scheduling and re-scheduling performed sequentially. evaluate optimize interaction...
Abstract Background Tandem mass spectrometry-based database searching has become an important technology for peptide and protein identification. One of the key challenges in is remarkable increase computational demand, brought about by expansion databases, semi- or non-specific enzymatic digestion, post-translational modifications other factors. Some software tools choose indexing to accelerate processing. However, requires a large amount time space construction, especially digestion....
Abstract Protein sequence database search based on tandem mass spectrometry is an essential method for protein identification. As the computational demand increases, parallel computing has become important technique accelerating proteomics data analysis. In this paper, we discuss several factors which could affect runtime of pFind engine and build estimation model. Based model, effective on‐line off‐line scheduling methods were developed. An experiment public dataset from PhosphoPep...
The CLLC converter is a promising topology for bidirectional power conversion applications, such as vehicle-to-grid (V2G) systems and battery energy storage systems. In these wide output voltage range needed. However, traditional pulse frequency modulation (PFM) control not suitable applications because of its switching range. this paper, novel inner phase shift (IPS) proposed converters. By modulating the IPS angle between primary-side bridge legs, can be regulated with variation (voltage...
Liquid chromatography-mass spectrometry (LC-MS) is the mainstream of high throughput protein identification technology. Peptide retention time in reversed-phase liquid chromatography (RPLC) mainly determined by physicochemical properties peptide and LC conditions (stationary phase mobile phase). Retention can be predicted analyzing these quantifying their effects on chromatographic behavior. Prediction used to improve peptides post translational modifications (PTM). There are two methods...
The CLLC resonant converters have huge potential in battery charging applications. In order to reduce the conduction loss, synchronous rectification (SR) technique is widely applied. However, conventional SR method ignores dead time resonance and thus leads hard turn-on of switches secondary side, which especially harmful light load conditions. this paper, a novel with switching delay for proposed, appropriate derived. proposed can realize soft side while maintaining benefits SR. Experiments...
With the rapid development of high throughput DNA sequencing,genomes more and species have been sequenced.Identifying determining structures coding genes are basic tasks genome annotation.To understand sequenced precisely,it is necessary to integrate muilti-omics data annotate genomes.However,the annotation methods developed in past decade mainly based on transcriptome data.Recently,mass spectrometry proteomics has come age,which can cover proteomes nearly completely make it possible use...
Frequency-domain thermal impedance model has been proved to be able predict more complex behaviors of power semiconductor devices under multi-timescales. But the essential prerequisite for accurate prediction is that key parameters this model, especially critical frequencies, are correctly characterized. In paper, impacts potential boundary conditions on frequency-domain comprehensively investigated through experimental measurements. The testing set different levels power-losses in chip, as...
The building air permeability plays a major role in both energy performance and indoor quality. of structural tightness is set as an evaluation index permeability, which employed to analyze the relationship among construction window/door permeability. Based on some assumptions, coefficient established summarize influential factors theory method provided improve A model for small room taken example. results indicate that available