A5100456787- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Soybean genetics and cultivation
- Plant Molecular Biology Research
- Nematode management and characterization studies
- Agronomic Practices and Intercropping Systems
- Influenza Virus Research Studies
- Animal Disease Management and Epidemiology
- Plant Virus Research Studies
- Yeasts and Rust Fungi Studies
- Photosynthetic Processes and Mechanisms
- Plant responses to water stress
- Plant Disease Resistance and Genetics
- Plant Stress Responses and Tolerance
- Plant pathogens and resistance mechanisms
- Identification and Quantification in Food
- Plant Reproductive Biology
- Insect-Plant Interactions and Control
- Phytase and its Applications
- RNA Research and Splicing
- Seed Germination and Physiology
- Plant Gene Expression Analysis
- CRISPR and Genetic Engineering
- Plant Micronutrient Interactions and Effects
- Genetic Mapping and Diversity in Plants and Animals
Oil Crops Research Institute
2015-2024
Chinese Academy of Agricultural Sciences
2014-2024
Ministry of Agriculture and Rural Affairs
2019-2024
Fujian Agriculture and Forestry University
2018-2021
Qufu Normal University
2017
University of Southern California
2016
Harbin Veterinary Research Institute
2004-2013
Chinese Academy of Sciences
2009
Huazhong Agricultural University
2009
Max Planck Institute for Molecular Genetics
2007
The pathogenicity of avian H5N1 influenza viruses to mammals has been evolving since the mid-1980s. Here, we demonstrate that viruses, isolated from apparently healthy domestic ducks in mainland China 1999 through 2002, were becoming progressively more pathogenic for mammals, and present a hypothesis explaining mechanism this evolutionary direction. Twenty-one southern 2002 confirmed be subtype A viruses. These isolates are antigenically similar A/Goose/Guangdong/1/96 (H5N1) virus, which was...
Wild waterfowl are the natural reservoir of all influenza A viruses, and these viruses usually nonpathogenic in birds. However, since late 2002, H5N1 outbreaks Asia have resulted mortality among recreational parks, domestic flocks, wild migratory The evolutionary stasis between virus its host may been disrupted, prompting us to ask whether resistant disease they can still act as a for viruses. To better understand biology ducks attempt answer this question, we inoculated juvenile mallards...
The plant architecture has significant effects on grain yield of various crops, including soybean (Glycine max), but the knowledge optimization in order to increase potential is still limited. Recently, CRISPR/Cas9 system revolutionized genome editing, and been widely utilized edit genomes a diverse range crop plants.In present study, we employed mutate four genes encoding SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors SPL9 family soybean. These GmSPL9 are negatively...
MYB transcription factors (TFs) have been reported to regulate the biosynthesis of secondary metabolites, as well mediate plant adaption abiotic stresses, including drought. However, roles TFs in regulating architecture and yield potential remain poorly understood. Here, we studied dehydration-inducible GmMYB14 gene architecture, high-density drought tolerance through brassinosteroid (BR) pathway soybean. was shown localize nucleus has a transactivation activity. Stable...
GATA transcription factors are transcriptional regulatory proteins that contain a characteristic type-IV zinc finger DNA-binding domain and recognize the conserved motif in promoter sequence of target genes. Previous studies demonstrated plant possess critical functions developmental control responses to environment. To date, soybean (Glycine max) have yet be characterized. Thus, this study identified 64 putative from entire genomic sequence. The chromosomal distributions, gene structures,...
Leaf-chewing insects are important pests that cause yield loss and reduce seed quality in soybeans (Glycine max). Breeding soybean varieties resistant to leaf-chewing can minimize the need for insecticide use loss. The marker gene QTL-M, Glyma.07g110300 (LOC100775351) encodes a UDP-glycosyltransferase (UGT) is major determinant of resistance against soybean; it exhibits function insect-resistant germplasms. In this study, Agrobacterium-mediated transformation introduced CRISPR/Cas9...
Whole-genome bisulfite sequencing (WGBS) has emerged as the gold-standard technique in genome-scale studies of DNA methylation. Mapping reads from WGBS requires unique considerations that make process more time-consuming than other applications. Typical data sets contain several hundred million reads, adding to this analysis challenge. We present WALT tool for mapping reads. uses a strategy hashing periodic spaced seeds, which leads significant speedup compared with most efficient methods...
Root nodule symbiosis (RNS) is one of the most productive and economical systems for nitrogen fixation, previous studies have shown that several nodule-specific C2H2-zinc finger proteins (ZFPs) play important roles in establishment function. However, C2H2-ZFPs are widespread ZFPs eukaryotes, a great variation structure function exist among family members. It remains largely unclear whether or not special types C2H2-ZF genes participate symbiosis, especially soybean. In present study, we...
Real-time quantitative reverse transcription PCR is a sensitive and widely used technique to quantify gene expression. To achieve reliable result, appropriate reference genes are highly required for normalization of transcripts in different samples. In this study, 9 previously published (60S, Fbox, ELF1A, ELF1B, ACT11, TUA5, UBC4, G6PD, CYP2) soybean [Glycine max (L.) Merr.] were selected. The expression stability the was evaluated under conditions biotic stress caused by infection with...
Soybean (Glycine max) is a legume crop with great economic value that provides rich protein and oil for human food animal feed. In order to cope the ever-increasing need soybean products changing environment, genetic improvement needs be accelerated. recent years, rapid developed genome editing technologies, such as zinc finger nuclease (ZFNs), transcription activator-like effector nucleases (TALENs), clustered regularly interspaced short palindromic repeats/CRISPR associated (CRISPR/Cas),...
Abstract Asian soybean rust (ASR), caused by Phakopsora pachyrhizi , is a devastating disease that present in all major soybean-producing regions. The limited availability of resistant germplasm has resulted scarcity commercial cultivars are to the disease. To date, only Chinese landrace SX6907 demonstrated an immune response ASR. In this study, we isolation and characterization Rpp6907-7 Rpp6907-4 gene pair confer broad-spectrum resistance encode atypic nucleotide-binding leucine-rich...
The root nodule symbiosis (RNS) between legume plants and rhizobia is the most efficient productive source of nitrogen fixation, has critical importance in agriculture mesology. Soybean (Glycine max), one important crops world, establishes a nitrogen-fixing with different types rhizobia, efficiency symbiotic fixation soybean greatly depends on host-specificity. Although, it been reported that use surface polysaccharides, secretion proteins type-three systems nod factors to modulate host...
Chlorophyll plays an essential role in photosynthetic light harvesting and energy transduction green tissues of higher plants is closely related to photosynthesis crop yield. Identification transcription factors (TFs) involved regulating chlorophyll biosynthesis still limited soybean (Glycine max), the previously identified GmGATA58 suggested potentially modulate nitrogen metabolisms, but its complete function unknown. In this study, subcellular localization assay showed that was localized...
The macronutrient potassium (K) is essential to plant growth and development. Crop yield potential often affected by lack of soluble K. molecular regulation mechanism physiological biochemical responses K starvation in soybean roots shoots not fully understood. In the present study, two varieties were subjected low-K stress conditions: a low-K-tolerant variety (You06-71) low-K-sensitive (HengChun04-11). Eight libraries generated for analysis: 2 genotypes ×2 tissues (roots shoots) time...
Soybean (Glycine max) is one of the most important economic legume crops with largest planting area, and also an oil crop, as well food feed material. Soybean-rhizobia symbiosis plays roles in plant cultivation fertilizer application. However, there are many problems agricultural application soybean symbiotic nitrogen fixation. In this review, we summarized three restriction factors (host specificity, low fixation efficiency abiotic stress) discussed research progresses these factors....
Nitrogen is an important macronutrient required for plant growth, and a limiting factor crop productivity. Improving the nitrogen use efficiency (NUE) therefore crucial. At present, NUE mechanism unclear information on genes associated with in soybeans lacking. cystathionine beta synthase (CBS) domain-containing proteins (CDCPs) may be implicated abiotic stress tolerance plants. We identified classified CBS protein superfamily soybean. A candidate gene NUE, GmCBS21, was identified. GmCBS21...
To deepen the knowledge about epidemic prevalence in goose breeding field, a triplex PCR assay was established, and 478 samples were collected from scaled farms 11 provinces China. The results of this epidemiological investigation showed that incidence rates H9 avian influenza circovirus highest among five infectious diseases evaluated. In addition, established remarkable sensitivity, rapidity versatility compared to other diagnostic methods. Dual infection comprised large proportion...