A5101914226- Gene expression and cancer classification
- Cancer-related molecular mechanisms research
- Phosphodiesterase function and regulation
- Bioinformatics and Genomic Networks
- Molecular Biology Techniques and Applications
- Genetic and phenotypic traits in livestock
- Cholinesterase and Neurodegenerative Diseases
- Geomagnetism and Paleomagnetism Studies
- Retinoids in leukemia and cellular processes
- Genetics, Aging, and Longevity in Model Organisms
- Bayesian Methods and Mixture Models
- Magnetic and Electromagnetic Effects
- Plant-Microbe Interactions and Immunity
- Peroxisome Proliferator-Activated Receptors
- Telomeres, Telomerase, and Senescence
- Heavy Metal Exposure and Toxicity
- Cardiac Ischemia and Reperfusion
- Adipokines, Inflammation, and Metabolic Diseases
- Yeasts and Rust Fungi Studies
- Electrohydrodynamics and Fluid Dynamics
- Plant biochemistry and biosynthesis
- Mercury impact and mitigation studies
- Fungal Plant Pathogen Control
- Amino Acid Enzymes and Metabolism
- 14-3-3 protein interactions
Syngenta (United States)
2024
University of Alabama at Birmingham
2014-2016
University of Wisconsin–Madison
2010-2014
Morgridge Institute for Research
2013
GlaxoSmithKline (United Kingdom)
2006-2009
Wellcome Trust
1986-1992
Messenger RNA expression is important in normal development and differentiation, as well manifestation of disease. RNA-seq experiments allow for the identification differentially expressed (DE) genes their corresponding isoforms on a genome-wide scale. However, statistical methods are required to ensure that accurate identifications made. A number exist identifying DE genes, but far fewer available isoforms. When isoform interest, investigators often apply gene-level (count-based) directly...
Bacteria in the genus Ruminococcus are ubiquitous members of mammalian gastrointestinal tract. In particular, they important ruminants where digest a wide range plant cell wall polysaccharides. For example, albus 7 is primary cellulose degrader that produces acetate usable by its bovine host. Moreover, it one few organisms ferments to form ethanol at mesophilic temperatures vitro. The mechanism degradation R. not well-defined and thought involve pilin-like proteins, unique...
Oral phosphodiesterase (PDE) 4 inhibitors such as roflumilast have established the potential of PDE4 inhibition for treatment respiratory diseases. However, inhibitor efficacy is limited by mechanism-related side effects emesis and nausea. Delivering inhaled route may improve therapeutic index, we describe 6-({3-[(dimethylamino)carbonyl]phenyl}sulfonyl)-8-methyl-4-{[3-methyloxy) phenyl]amino}-3-quinolinecarboxamide (GSK256066), an exceptionally high-affinity designed administration....
Abstract Asian soybean rust (ASR), caused by Phakopsora pachyrhizi , is a devastating disease that present in all major soybean-producing regions. The limited availability of resistant germplasm has resulted scarcity commercial cultivars are to the disease. To date, only Chinese landrace SX6907 demonstrated an immune response ASR. In this study, we isolation and characterization Rpp6907-7 Rpp6907-4 gene pair confer broad-spectrum resistance encode atypic nucleotide-binding leucine-rich...
A common goal of microarray and related high-throughput genomic experiments is to identify genes that vary across biological condition. Most often this accomplished by identifying with changes in mean expression level, so called differentially expressed (DE) genes, a number effective methods for DE have been developed. Although useful, these approaches do not accommodate other types differential regulation. An important example concerns coexpression (DC). Investigations class are hampered...
Abstract Summary: R/EBcoexpress implements the approach of Dawson and Kendziorski using R, a freely available, open source statistical programming language. The identifies differential co-expression (DC) by examining correlations among gene pairs an empirical Bayesian approach, producing false discovery rate controlled list DC pairs. This interrogation complements but is distinct from expression analyses, under general goal understanding regulation across biological conditions. Availability...
We previously reported that mice with skin-specific deletion of stearoyl-CoA desaturase-1 (Scd1) recapitulated the skin phenotype and hypermetabolism observed in a whole-body Scd1. In this study, we first performed diet-induced obesity experiment at thermoneutral temperature (33°C) found Scd1 knockout (SKO) still remain resistant to obesity. To elucidate metabolic changes contribute resistance phenotype, microarray analysis gene expression male SKO control fed standard rodent diet....
Abstract Identifying the genetic basis of complex traits remains an important and challenging problem with potential to affect a broad range biological endeavors. A number statistical methods are available for mapping quantitative trait loci (QTL), but their application high-throughput phenotypes has been limited as most require user input interaction. Recently, have developed specifically expression QTL (eQTL) mapping, they too in that do not allow interactions moderate effect. We here...
OAG‐stimulated superoxide (O − 2 ) production by HL‐60 granulocytes showed enantiomeric specificity but reached a maximum of only 5% that produced either phorbol myristate acetate (PMA) or dibutyrate (PDBu). At 10–100 μM, OAG displaced specifically‐bound [ 3 H]PDBu from intact cells 25%, suggesting limited cell penetration. (10–100 μM) also inhibited PDBu‐stimulated O 25%; this inhibition was enantiomerically specific. However, at lower concentration (3 μM), both enantiomers fully blocked...
Two challenging problems in the clinical study of cancer are characterization subtypes and classification individual patients according to those subtypes. Statistical approaches addressing these hampered by population heterogeneity challenges inherent data integration across high-dimensional, diverse covariates. We have developed a survival-supervised latent Dirichlet allocation (survLDA) modeling framework address concerns. LDA models proven extremely effective at identifying themes common...
1,2-sn-Dihexanoylglycerol (HHG) reduced the ATP content of HL-60 cells. This concentration-related (10-100 microM) effect reached a maximum over 90%, was enantiomerically specific and not accompanied by release lactate dehydrogenase. Oleoylacetylglycerols (3-100 had no on levels while phorbol dibutyrate (PDBu, 0.01-1 decreased cells up to 40%. Responses stimulated HHG became limited as concentration increased above 10 microM, this being manifest either low response compared PDBu (superoxide...
The order of potency retinoids as inhibitors A23187-induced production leukotriene B4 (LTB4) in human polymorphonuclear leukocytes (PMN) was retinoic acid greater than retinal retinol. However, the conversion exogenous arachidonate (AA) to LTB4 by PMN homogenates inhibited rank retinol much acid. agreement between active concentrations these two systems is consistent with this compound acting directly inhibit AA metabolism: not so for other retinoids. inhibition phorbol dibutyrate...
In an early paper on geomagnetic micropulsations, based data from Sodankyla, Sucks-dorff [1936] reported Pc 1's (regular pulsations with period between 0.2 and 5 seconds) to be essentially daytime phenomenon occurrence maximum about 1400 local time. Much later Ts were considered nocturnal [Benioff, 1960; Tepley Wentworth, 1962] the basis of studies made at middle latitudes. Heacock Hessler [1962] restated fact that in auroral latitudes, occur primarily during day. Campbell Stiltner [1965]...
Genomic-based studies of disease now involve diverse types data collected on large groups patients. A major challenge facing statistical scientists is how best to combine the data, extract important features, and comprehensively characterize ways in which they affect an individual's course likelihood response treatment. We have developed a survival-supervised latent Dirichlet allocation (survLDA) modeling framework address these challenges. Latent (LDA) models proven extremely effective at...
That one or multiple measures of metabolic rate may be robustly associated with, possibly even causative of, the progression aging-resultant phenotypes such as lifespan is a long-standing, well-known mechanistic hypothesis. To broach this hypothesis, we assessed function and spontaneous locomotion in two genetic dietary mouse models for retarded aging, subjected data to mediation analyses determine whether any locomotor trait could identified mediator effect interventions on senescence. We...