A5021038249- Cancer therapeutics and mechanisms
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Biochemical and Molecular Research
- Photosynthetic Processes and Mechanisms
- Retinal Development and Disorders
- Photochromic and Fluorescence Chemistry
- Synthesis and biological activity
- Complex Network Analysis Techniques
- Sports Analytics and Performance
- Histone Deacetylase Inhibitors Research
- DNA and Nucleic Acid Chemistry
- Antibiotic Resistance in Bacteria
- Lung Cancer Research Studies
- Bioactive Compounds and Antitumor Agents
- Mosquito-borne diseases and control
- Evolutionary Game Theory and Cooperation
- Plant tissue culture and regeneration
- Biocrusts and Microbial Ecology
- Genetics, Aging, and Longevity in Model Organisms
- Plant Virus Research Studies
- Epigenetics and DNA Methylation
- Plant and Biological Electrophysiology Studies
- Plant Reproductive Biology
- DNA Repair Mechanisms
Broad Institute
2019-2022
Yangzhou University
2017-2021
Shanghai Jiao Tong University
2020
Brigham and Women's Hospital
2020
Harvard University
2020
Florida International University
2015-2018
Escherichia coli topoisomerase I has an essential function in preventing hypernegative supercoiling of DNA. A full length structure E. reported here shows how the C-terminal domains bind single-stranded DNA (ssDNA) to recognize accumulation negative supercoils duplex These are known interact with RNA polymerase, and two flexible linkers within may assist movement ssDNA for rapid removal transcription driven supercoils. The also unveiled first time 4-Cys zinc ribbon domain ribbon-like...
Several genome engineering applications of CRISPR-Cas9, an RNA-guided DNA endonuclease, require precision control Cas9 activity over dosage, timing, and targeted site in organism. While some dose time have been achieved using small molecules, spatial light, no singular system with all the three attributes exists. Furthermore, reported small-molecule systems lack wide dynamic range, background absence controller, are not biologically inert, while optogenetic prolonged exposure to...
Escherichia coli topoisomerase I (TopA), a regulator of global and local DNA supercoiling, is modified by Nε-Lysine acetylation. The NAD+-dependent protein deacetylase CobB can reverse both enzymatic non-enzymatic lysine acetylation modification in E. coli. Here, we show that the absence ΔcobB mutant reduces intracellular TopA catalytic activity increases negative supercoiling. expression level elevated as topA transcription responds to increased slow growth phenotype be partially...
Prolonged Cas9 activity can hinder genome engineering as it causes off-target effects, genotoxicity, heterogeneous genome-editing outcomes, immunogenicity, and mosaicism in embryonic editing—issues which could be addressed by controlling the longevity of Cas9. Though some temporal controls have been developed, only cumbersome systems exist for modifying lifetime. Here, we developed a chemogenetic system that brings proximity to ubiquitin ligase, enabling rapid ubiquitination degradation...
Agrobacterium tumefaciens is the agent that causes crown gall tumor disease on more than 140 species of dicotyledonous plants. Chemotaxis A. toward wound sites host plant first step to recognize host. CheW a coupling protein bridges histidine kinase CheA and chemoreceptors form chemotaxis core signaling complex plays crucial role in assembly function large chemosensory array. Unlike all previously reported systems, has only one major che operon but two cheW homologs (atu2075 as cheW1 atu2617...
The relaxation activity of E. coli topoisomerase I is required for regulation global and local DNA supercoiling. in vivo enzyme sensitive to lysine acetylation–deacetylation can affect supercoiling growth as a result. Nonenzymatic acetylation by acetyl phosphate has been shown reduce the I. In this work, biochemical consequence modification with enzymatic assays was studied. Results showed that noncovalent binding cleavage were reduced result acetylation, greater effect on cleavage. Four...
Chemoreceptor (also called methyl-accepting chemotaxis protein, MCP) is the leading signal protein in signaling pathway. MCP senses and binds chemoeffectors, specifically, transmits sensed to downstream proteins of system. The genome Agrobacterium fabrum (previously, tumefaciens) C58 predicts that a total 20 genes can encode MCP, but only MCP-encoding gene atu0514 located inside che operon. Hence, identification exact function atu0514-encoding chemoreceptor (here, named as MCP514) will be...
Abstract Several genome engineering applications of CRISPR‐Cas9, an RNA‐guided DNA endonuclease, require precision control Cas9 activity over dosage, timing, and targeted site in organism. While some dose time have been achieved using small molecules, spatial light, no singular system with all the three attributes exists. Furthermore, reported small‐molecule systems lack wide dynamic range, background absence controller, are not biologically inert, while optogenetic prolonged exposure to...
Non-profit sports organizations play a more important role in our society than ever before, as the public is now aware of their personal health and have recognized importance community for persistent sport exercise. The long-term interactions members such allow researchers to observe structure from perspective dynamic social network. With this paper, we study formation non-profit organization, with data collected over four years. Our main goal understand dynamics find how affected by...