Astaxanthin is a high-value red pigment and antioxidant widely used in the pharmaceutical, cosmetic, food industries. However, hydrophobicity of astaxanthin causes its low bioavailability. Glycosylation can substantially increase water solubility astaxanthin, thus enhancing bioavailability, photostability, biological activities. In this study, we report for first time heterologous production glycosylated Yarrowia lipolytica. By appropriate removal chloroplast transit peptide, carotenoid...

Zeaxanthin is a carotenoid, dihydroxy derivative of β-carotene. has antioxidant, anti-inflammatory, anticancer, and neuroprotective properties. In this study, Yarrowia lipolytica was used as host for the efficient production zeaxanthin. The strain Y. PO1h to construct following engineered strains carotenoid since it produced highest β-carotene among PO1h- PEX17-HA-derived strains. By regulating key nodes on pathway through wild mutant enzyme comparison successive modular assembly,...

This paper proposes an interactive system based on spectrogram analysis, deep neural network and wavelet analysis aiming at the complexity subjectivity of music emotion recognition. The first uses a to capture time-frequency characteristics signals then automatically extracts emotion-related features through convolutional (CNN). introduces Mallat algorithm for decomposition enhance local details audio improve accuracy feature extraction. experimental results show that performs well in...

Mobile platforms, such as Android, warn users about the permissions an app requests and trust that user will make correct decision whether or not to install app. Unfortunately many either ignore warning fail understand risks they imply. As a step toward developing indicator of risk decomposes into several categories, dimensions, we conducted two studies designed assess dimensions deemed most important by experts novices. In Study 1, semi-structured interviews were with 19 security experts,...

The levels of dissolved sulfate and methane are crucial indicators in the geochemical analysis pore water. Compositional water samples obtained from sea trials was conducted using Raman spectroscopy. It found that concentration SO42− decreases as depth increases, while expected signal has not been observed. A possible reason for this is escaped after sampling remaining too low to be detected. To find more effective ways analyze composition water, two novel approaches proposed. One based on...

A newly developed integrated fluorescence-Raman spectral system (λex = 532 nm) for detecting Chlorophyll-a (chl-a), Chromophoric Dissolved Organic Matter (CDOM), carotenoids and SO₄(2-) in situ was used to successfully investigate the diurnal variability of all above. Simultaneously using integration fluorescence spectroscopy Raman techniques provided comprehensive marine information due complementarity between different excitation mechanisms selection rules. The investigation took place...

Amplitude modulation high-frequency (HF) radio waves at extremely low frequencies (ELF, 0.3-3 kHz) or very (VLF, 3-30 kHz), heating the ionosphere, can nonlinearly modulate auroral electrojet to produce ELF/VLF for long-distance communication. This article presents results of first communication experiments being conducted Tromsø, where European Incoherent Scatter Scientific Association (EISCAT) HF facility is located. The from ionosphere are modulated by quaternary phase shift keying (QPSK)...

When a hypersonic vehicle flies in the atmosphere, plasma sheath and trails are configured around due to aerodynamic heating ablation, which distort radar echo signal then influence target range information Doppler frequency extracted from echoes. In order examine impact of laminar wake on detection, this article is primarily aimed establish model based linear frequency-modulated continuous wave (LFMCW) radar, takes into account reaction control system by employing Born approximation...

Front surface texturing is a common method used to improve the optical performance of photovoltaic devices. However, traditional techniques may be challenging in some cases, such as when dealing with ultra-thin substrates. Textured polymer films on devices would an alternative approach. This paper reports study NOA81 thin bionic lotus leaf structure monocrystalline silicon solar cells. Inspired by natural leaves, we successfully prepared microstructure film cells based using...

Recently, LSTSVM as a new binary SVM classifier based on nonparallel twin hyperplanes has shown good classification performance, but the research multi-class still rarely been reported. In this paper, optimal directed acyclic graph is proposed. The idea of kernel parameter choice used to realize class separability criterion, an average distance measure and non-repetitive sequence number rearrangement method are offered in order reduce cumulative errors caused by DAG structure. experimental...

In this study, mixtures of different kinds bentonite and soil were used tested in order to find a cheap alternative current anti-seepage materials for constructed wetlands. The layer wetlands was simulated the experimental study permeability coefficient mixed determined evaluate effect mixtures. main results are as follows: (i) minimum mass ratio is 10%; (ii) Within certain range, more compact higher wet density is, then better (under condition moisture content). exponentially increased with...

Based on the projections of three shared socioeconomic pathways (SSPs) scenarios climate models CMIP6, this study analyzed standardized precipitation evapotranspiration index (SPEI) to understand future meteorological dryness/wetness changes in Poyang Lake basin (PLB) from 2021 2100. The effect temperature change dryness and wetness variation was explored by comparing trends SPEI (SPI) at multiple-time scales different SSPs scenarios. results indicate that frequency drought events may...

A rapid and simple method was commonly used for the determination of microcystins by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The reliability, accuracy sensitivity LC-MS/MS were affected matrix effect because complex matrices in algal bloom samples. In this study, interference problems effectively reduced or eliminated diluting samples, optimizing gradient elution programs, reducing injection volumes. correlation coefficients (r2) seven ≥ 0.99. limits...

Parametric decay instability (PDI) generated in milliseconds is an important physical phenomenon ionospheric heating. Usually, numerical simulations are used to study PDI mechanisms. They can intuitively investigate the generation and development process of PDI, which necessary experimental studies. When simulating through explicit finite-difference time-domain (FDTD), spatial scale spans from kilometers centimeters, time needs meet Courant–Friedrichs–Lewy condition. Simulating...

A free-air ionization chamber in low-energy X-ray has been designed and manufactured at the National Institute of Metrology (NIM, China) according to defination air-kerma. The results a preliminary test show that leakage current is around 2×10−15A, correction factor ion recombination for also obtained. suitable primary standard X-rays.