A5101786237- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Acupuncture Treatment Research Studies
- Cardiac Arrest and Resuscitation
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Pain Mechanisms and Treatments
- Complementary and Alternative Medicine Studies
- Biochemical and Structural Characterization
- Pain Management and Placebo Effect
- Cannabis and Cannabinoid Research
- Dermatology and Skin Diseases
- Allergic Rhinitis and Sensitization
- Coronary Interventions and Diagnostics
- Hypothalamic control of reproductive hormones
- Cardiac, Anesthesia and Surgical Outcomes
- Hormonal Regulation and Hypertension
- Psychosomatic Disorders and Their Treatments
- Pharmacological Receptor Mechanisms and Effects
- Vascular Procedures and Complications
- Dietary Effects on Health
- Cardiac electrophysiology and arrhythmias
- Exercise and Physiological Responses
- Nausea and vomiting management
- Renal function and acid-base balance
- Pediatric Pain Management Techniques
Endeavour College of Natural Health
2023-2025
Griffith University
2013-2023
Leicester Royal Infirmary
2004-2021
University of Leicester
2006-2021
Providence Center
2017
Wiltshire and Swindon History Centre
2016
East Lancashire Hospitals NHS Trust
2016
Blackburn College
2014
Robarts Clinical Trials
2012
Royal Blackburn Teaching Hospital
2009
The compound SB-612111 [(-)-cis-1-methyl-7-[[4-(2,6-dichlorophenyl)piperidin-1-yl]methyl]-6,7,8,9-tetrahydro-5H-benzocyclohepten-5-ol] was recently identified as a selective antagonist for the nociceptin/orphanin FQ (N/OFQ) peptide receptor (NOP). In present study, in vitro pharmacological profile of at human recombinant NOP receptors expressed Chinese hamster ovary (CHO) cells [receptor binding, guanosine 5'-O-(3-[(35)S]thio)triphosphate (GTPgamma[(35)S]) and cAMP level experiments] well...
Real-time quantitative RT–PCR (qRT–PCR) is a powerful technique used for the relative quantification of target genes, using reference (housekeeping) genes normalization to ensure generation accurate and robust data. A systematic examination suitability endogenous gene expression studies in endometrial cancer tissues absent. The aims this study were therefore identify evaluate from thirty-two possible TaqMan® array panel their as an internal control gene. mathematical software packages geNorm...
Partial agonism is primarily dependent upon receptor density and coupling efficiency. As these parameters are tissue/model dependent, intrinsic activity in different tissues can vary. We have utilised the ecdysone‐inducible expression system containing human nociceptin/orphanin FQ (N/OFQ) peptide (hNOP) expressed Chinese hamster ovary cells (CHO INDhNOP ) to examine of a range partial agonists binding, GTP γ 35 S binding inhibition adenylyl cyclase studies. Incubation CHO with ponasterone A...
This study investigates the mechanisms by which muscarinic receptor gene family can protect against apoptosis. Chinese hamster ovary cells transfected with human subtypes underwent apoptotic cell death following treatment DNA-damaging agent etoposide. Apoptosis was significantly reduced stimulation of that were couple to Gq/11/phospholipase C pathway, namely M1, M3, and M5. No protection detected in Gi-coupled M2 M4 receptors. Further analysis Gq/11-coupled M3 revealed truncation...
Expression of opioid receptors on peripheral blood mononuclear cells (PBMC) is controversial. These are currently classified as classical (MOP/mu/mu, DOP/delta/delta and KOP/kappa/kappa) nonclassical NOP (nociceptin/orphanin FQ; N/OFQ). In this volunteer study we probed for the expression both using 1) radioligand binding, 2) specific antibody 3) polymerase chain reaction-based experimental paradigms. Membranes prepared from PBMC healthy volunteers did not bind either [3H]diprenorphine (a...
Subclinical inflammation is frequently associated with obesity. Here, we aim to better define the acute inflammatory response during fasting. To do so, analyzed representatives of immune-related proteins in circulation and tissues as potential markers for adipose tissue modulation immune system. Lipopolysaccharide treatment or high-fat diet led an increase circulating serum amyloid (SAA) α1-acid glycoprotein (AGP), whereas adipsin levels were reduced. Mouse models that are protected against...
Intrathecally (i.t.) administered nociceptin/orphanin FQ (N/OFQ) evokes antinociceptive effects in rodents. Recent studies monkeys demonstrated that i.t. co-application of N/OFQ and morphine elicits synergistic actions suggesting mixed peptide (NOP) μ opioid receptor agonists as innovative spinal analgesics. Thus, novel related peptides were synthesized order to identify pharmacologically characterize a NOP/ agonist.The following vitro assays used: calcium mobilization cells expressing the...
Introduction Traditionally, patients are kept nil-per-os/nil-by-mouth (NPO/NBM) prior to invasive cardiac procedures, yet there exists neither evidence nor clear guidance about the benefits of this practice. Objectives To demonstrate that percutaneous catheterisation does not require fasting. Methods The data source is a retrospective analysis registry consecutive who underwent coronary intervention (PCI) for acute syndrome (ACS) and stable angina at two district general hospitals in UK with...
Accumulating evidence highlights the importance of endocannabinoid anandamide (AEA) as a key mediator in reproductive physiology. Current data suggest potential roles for AEA gametogenesis, fertilization, and parturition. exerts its actions through two G protein-coupled receptors, termed cannabinoid receptor 1 (CB1), 2 (CB2), ligand-gated transient vanilloid type (TRPV1) ion channel. At present, cellular mechanism(s) consequences signaling tissues, especially myometrium, are poorly...
Background And ObjectivesNociceptin/Orphanin FQ (N/OFQ) is a non-classical endogenous opioid peptide that modulates immune function in vitro. Its importance inflammation and human sepsis unknown. The objectives of this study were to determine the relationship between N/OFQ, transcripts for its precursor (pre-pro-N/OFQ [ppNOC]) receptor (NOP), inflammatory markers clinical outcomes patients undergoing cardiopulmonary bypass with sepsis. MethodsA prospective observational cohort 82 admitted...